Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance
The CCCH zinc finger proteins play critical roles in a wide variety of growth, development, and stress responses. Currently, limited reports are available about the roles of animal CCCH proteins in plants. In this study, we report the identification of human TTP (hTTP) with functional similarity to...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2024-01-01
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| Series: | Forestry Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/forres-0024-0008 |
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| author | Yamei Zhuang Yang Chen Qiao Wang Yan Chen Liping Yan Shengjun Li Gongke Zhou Guohua Chai |
| author_facet | Yamei Zhuang Yang Chen Qiao Wang Yan Chen Liping Yan Shengjun Li Gongke Zhou Guohua Chai |
| author_sort | Yamei Zhuang |
| collection | DOAJ |
| description | The CCCH zinc finger proteins play critical roles in a wide variety of growth, development, and stress responses. Currently, limited reports are available about the roles of animal CCCH proteins in plants. In this study, we report the identification of human TTP (hTTP) with functional similarity to PdC3H17 in a hybrid poplar. hTTP and PdC3H17 shared highly similar tandem CCCH zinc-finger RNA-binding domains. The fragments excluding the CCCH domain of both hTTP and PdC3H17 possessed transcriptional activation activities in yeast cells. Compared to the controls, ectopic expression of hTTP in poplar caused dwarfism, and resulted in significant increases in stem xylem vessel number and photosynthetic and ROS-scavenging abilities, thereby enhancing plant tolerance to drought stress. Our results suggest that hTTP may perform a function in poplar through the PdC3H17-mediated system, and provide an example for the application of animal genes in plants. |
| format | Article |
| id | doaj-art-9613e6892a1a4f849e52e914a97fec45 |
| institution | OA Journals |
| issn | 2767-3812 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Forestry Research |
| spelling | doaj-art-9613e6892a1a4f849e52e914a97fec452025-08-20T02:12:25ZengMaximum Academic PressForestry Research2767-38122024-01-014125125910.48130/forres-0024-0008forres-0024-0008Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought toleranceYamei Zhuang0Yang Chen1Qiao Wang2Yan Chen3Liping Yan4Shengjun Li5Gongke Zhou6Guohua Chai7College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, ChinaAcademy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying 257000, ChinaShandong Provincial Academy of Forestry, Jinan 250014, ChinaKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Energy Institute, Qingdao New Energy Shangdong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaAcademy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying 257000, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, ChinaThe CCCH zinc finger proteins play critical roles in a wide variety of growth, development, and stress responses. Currently, limited reports are available about the roles of animal CCCH proteins in plants. In this study, we report the identification of human TTP (hTTP) with functional similarity to PdC3H17 in a hybrid poplar. hTTP and PdC3H17 shared highly similar tandem CCCH zinc-finger RNA-binding domains. The fragments excluding the CCCH domain of both hTTP and PdC3H17 possessed transcriptional activation activities in yeast cells. Compared to the controls, ectopic expression of hTTP in poplar caused dwarfism, and resulted in significant increases in stem xylem vessel number and photosynthetic and ROS-scavenging abilities, thereby enhancing plant tolerance to drought stress. Our results suggest that hTTP may perform a function in poplar through the PdC3H17-mediated system, and provide an example for the application of animal genes in plants.https://www.maxapress.com/article/doi/10.48130/forres-0024-0008ccch proteinhttpdrought stressvessel cellros scavenging activitypopulus |
| spellingShingle | Yamei Zhuang Yang Chen Qiao Wang Yan Chen Liping Yan Shengjun Li Gongke Zhou Guohua Chai Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance Forestry Research ccch protein http drought stress vessel cell ros scavenging activity populus |
| title | Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| title_full | Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| title_fullStr | Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| title_full_unstemmed | Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| title_short | Ectopic expression of TTP gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| title_sort | ectopic expression of ttp gene from human in poplar promotes xylem differentiation and confers plant drought tolerance |
| topic | ccch protein http drought stress vessel cell ros scavenging activity populus |
| url | https://www.maxapress.com/article/doi/10.48130/forres-0024-0008 |
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