Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR
Background: Helicobacter pylori is a spiral-shaped bacterium that thrives in the digestive system. This bacterial infection is highly prevalent, it affects more than 50% of the world's population. It is common among children in developing countries. Transmission can occur through close persona...
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Zagazig University, Faculty of Medicine
2025-08-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_407491_babb7ea20b98a13606e8217eee270248.pdf |
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| author | Menna allah Mohamed Rania Abdel Halim Névine Kassem Eman kamel sally saber Engy Elsayed samar rashad |
| author_facet | Menna allah Mohamed Rania Abdel Halim Névine Kassem Eman kamel sally saber Engy Elsayed samar rashad |
| author_sort | Menna allah Mohamed |
| collection | DOAJ |
| description | Background: Helicobacter pylori is a spiral-shaped bacterium that thrives in the digestive system. This bacterial infection is highly prevalent, it affects more than 50% of the world's population. It is common among children in developing countries. Transmission can occur through close personal contact. Factors that increase the likelihood of infection include residing in densely populated areas with limited access to clean water sources. Gene mutations in Helicobacter pylori can lead to clarithromycin resistance by altering the bacterial ribosomal target site. These mutations, particularly in the 23S rRNA gene, prevent clarithromycin from binding, allowing the bacteria to survive in the stomach. Aim: This research seeks to identify point mutations linked to Helicobacter pylori's resistance to clarithromycin in stool samples through real-time PCR analysis, aiming to assist physicians in effectively treating patients infected with H. pylori. Methods: From December 2019 to May 2020, this cross-sectional investigation involved 90 patients exhibiting symptoms indicative of Helicobacter pylori infection. These individuals were examined at the endoscopy unit of Ain Shams University Hospitals' Hepatology, Gastroenterology, and Infectious Disease Department. Results: prevalence rate of clarithromycin resistance (46%), with A2143G accounting for 28% and A2142G for 18%. Conclusion: The study found a high rate of clarithromycin-resistant H. pylori (46%), mainly linked to the A2143G point mutation, and also identified the A2142G point mutation. Neither age nor gender showed a significant association with clarithromycin resistance. Given these findings, clarithromycin-based H. pylori treatment regimen may not be advisable as an empirical treatment approach in Egypt. |
| format | Article |
| id | doaj-art-9601dbe528ab4fd590fbf10afb2a8133 |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj-art-9601dbe528ab4fd590fbf10afb2a81332025-08-20T03:37:23ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402025-08-01633051306410.21608/mid.2025.337736.2447407491Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCRMenna allah Mohamed0Rania Abdel Halim1Névine Kassem2Eman kamel3sally saber4Engy Elsayed5samar rashad6Clinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptInternal Medicine Department, Faculty of Medicine, Ain Shams University, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, EgyptBackground: Helicobacter pylori is a spiral-shaped bacterium that thrives in the digestive system. This bacterial infection is highly prevalent, it affects more than 50% of the world's population. It is common among children in developing countries. Transmission can occur through close personal contact. Factors that increase the likelihood of infection include residing in densely populated areas with limited access to clean water sources. Gene mutations in Helicobacter pylori can lead to clarithromycin resistance by altering the bacterial ribosomal target site. These mutations, particularly in the 23S rRNA gene, prevent clarithromycin from binding, allowing the bacteria to survive in the stomach. Aim: This research seeks to identify point mutations linked to Helicobacter pylori's resistance to clarithromycin in stool samples through real-time PCR analysis, aiming to assist physicians in effectively treating patients infected with H. pylori. Methods: From December 2019 to May 2020, this cross-sectional investigation involved 90 patients exhibiting symptoms indicative of Helicobacter pylori infection. These individuals were examined at the endoscopy unit of Ain Shams University Hospitals' Hepatology, Gastroenterology, and Infectious Disease Department. Results: prevalence rate of clarithromycin resistance (46%), with A2143G accounting for 28% and A2142G for 18%. Conclusion: The study found a high rate of clarithromycin-resistant H. pylori (46%), mainly linked to the A2143G point mutation, and also identified the A2142G point mutation. Neither age nor gender showed a significant association with clarithromycin resistance. Given these findings, clarithromycin-based H. pylori treatment regimen may not be advisable as an empirical treatment approach in Egypt.https://mid.journals.ekb.eg/article_407491_babb7ea20b98a13606e8217eee270248.pdfkey words: molecular identificationhelicobacter pyloriresistance to clarithromycin |
| spellingShingle | Menna allah Mohamed Rania Abdel Halim Névine Kassem Eman kamel sally saber Engy Elsayed samar rashad Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR Microbes and Infectious Diseases key words: molecular identification helicobacter pylori resistance to clarithromycin |
| title | Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR |
| title_full | Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR |
| title_fullStr | Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR |
| title_full_unstemmed | Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR |
| title_short | Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR |
| title_sort | molecular detection of clarithromycin resistant helicobacter pylori in stool samples using real time pcr |
| topic | key words: molecular identification helicobacter pylori resistance to clarithromycin |
| url | https://mid.journals.ekb.eg/article_407491_babb7ea20b98a13606e8217eee270248.pdf |
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