Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)

This study was conducted to determine the dietary riboflavin requirement and its effects on growth performance, feed utilization, innate immunity, and diet digestibility of Litopenaeus vannamei. A riboflavin-free basal diet (R0) was formulated as a control, and six other diets were prepared by addin...

Full description

Saved in:
Bibliographic Details
Main Authors: Kokila Sanjeewani, Kyeong-Jun Lee
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Aquaculture Nutrition
Online Access:http://dx.doi.org/10.1155/2023/6685592
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832559707982659584
author Kokila Sanjeewani
Kyeong-Jun Lee
author_facet Kokila Sanjeewani
Kyeong-Jun Lee
author_sort Kokila Sanjeewani
collection DOAJ
description This study was conducted to determine the dietary riboflavin requirement and its effects on growth performance, feed utilization, innate immunity, and diet digestibility of Litopenaeus vannamei. A riboflavin-free basal diet (R0) was formulated as a control, and six other diets were prepared by adding riboflavin of 10, 20, 30, 40, 50, and 60 mg/kg to the basal diet (designated as R10, R20, R30, R40, R50, and R60, respectively). Quadruplicate groups of shrimp (initial average weight 0.17±0.00 g) were fed the diets six times a day for 8 weeks. Weight gain, specific growth rate, and protein efficiency ratio were significantly increased by riboflavin (p<0.05). The maximum values were observed in shrimp fed R40 diet. The highest activities of phenoloxidase, nitro blue tetrazolium, superoxide dismutase, and glutathione peroxidase were observed in shrimp fed R40 diet. Lysozyme activity was significantly higher in shrimp fed R30 and R40 diets than that of shrimp fed R60 diet (p<0.05). Intestinal villi were significantly longer in shrimp fed R50 and R60 diets compared to those of all other groups while the shortest villi were observed in R0 group (p<0.05). Intestinal villi were clearly distinguished in shrimp fed higher levels of riboflavin compared to those of shrimp fed R0 and R10 diets. Apparent digestibility coefficients of dry matter and protein in diets were not significantly affected by riboflavin levels (p<0.05). Whole-body proximate composition and hemolymph biochemical parameters were not significantly altered by dietary riboflavin (p<0.05). Therefore, the results of this study indicate that riboflavin is essential to enhance growth performance, feed utilization, nonspecific immunity, and intestine morphology of shrimp. An optimal riboflavin requirement for the maximum growth of L. vannamei seems to be approximately 40.9 mg/kg diet.
format Article
id doaj-art-95fe85388b134500b056e6d716037bc4
institution Kabale University
issn 1365-2095
language English
publishDate 2023-01-01
publisher Wiley
record_format Article
series Aquaculture Nutrition
spelling doaj-art-95fe85388b134500b056e6d716037bc42025-02-03T01:29:27ZengWileyAquaculture Nutrition1365-20952023-01-01202310.1155/2023/6685592Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)Kokila Sanjeewani0Kyeong-Jun Lee1Department of Marine Life SciencesDepartment of Marine Life SciencesThis study was conducted to determine the dietary riboflavin requirement and its effects on growth performance, feed utilization, innate immunity, and diet digestibility of Litopenaeus vannamei. A riboflavin-free basal diet (R0) was formulated as a control, and six other diets were prepared by adding riboflavin of 10, 20, 30, 40, 50, and 60 mg/kg to the basal diet (designated as R10, R20, R30, R40, R50, and R60, respectively). Quadruplicate groups of shrimp (initial average weight 0.17±0.00 g) were fed the diets six times a day for 8 weeks. Weight gain, specific growth rate, and protein efficiency ratio were significantly increased by riboflavin (p<0.05). The maximum values were observed in shrimp fed R40 diet. The highest activities of phenoloxidase, nitro blue tetrazolium, superoxide dismutase, and glutathione peroxidase were observed in shrimp fed R40 diet. Lysozyme activity was significantly higher in shrimp fed R30 and R40 diets than that of shrimp fed R60 diet (p<0.05). Intestinal villi were significantly longer in shrimp fed R50 and R60 diets compared to those of all other groups while the shortest villi were observed in R0 group (p<0.05). Intestinal villi were clearly distinguished in shrimp fed higher levels of riboflavin compared to those of shrimp fed R0 and R10 diets. Apparent digestibility coefficients of dry matter and protein in diets were not significantly affected by riboflavin levels (p<0.05). Whole-body proximate composition and hemolymph biochemical parameters were not significantly altered by dietary riboflavin (p<0.05). Therefore, the results of this study indicate that riboflavin is essential to enhance growth performance, feed utilization, nonspecific immunity, and intestine morphology of shrimp. An optimal riboflavin requirement for the maximum growth of L. vannamei seems to be approximately 40.9 mg/kg diet.http://dx.doi.org/10.1155/2023/6685592
spellingShingle Kokila Sanjeewani
Kyeong-Jun Lee
Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
Aquaculture Nutrition
title Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
title_full Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
title_fullStr Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
title_full_unstemmed Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
title_short Dietary Riboflavin Requirement of Pacific White Shrimp (Litopenaeus vannamei)
title_sort dietary riboflavin requirement of pacific white shrimp litopenaeus vannamei
url http://dx.doi.org/10.1155/2023/6685592
work_keys_str_mv AT kokilasanjeewani dietaryriboflavinrequirementofpacificwhiteshrimplitopenaeusvannamei
AT kyeongjunlee dietaryriboflavinrequirementofpacificwhiteshrimplitopenaeusvannamei