CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit
Abstract Background CRISPR/Cas9 technology has garnered increasing attention for its simplicity and precision in genome editing, making it an indispensable tool for gene function research and crop genetic improvement. However, the inefficiency and time-consuming nature of genetic transformation cont...
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| Language: | English |
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BMC
2025-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-07112-6 |
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| author | Xiaoyan Luo Ying Dou Yuxuan Lang Huamei Zhao Xinling Liu Xiaoli Zhang Yuxing Li Dong Liang Hui Xia |
| author_facet | Xiaoyan Luo Ying Dou Yuxuan Lang Huamei Zhao Xinling Liu Xiaoli Zhang Yuxing Li Dong Liang Hui Xia |
| author_sort | Xiaoyan Luo |
| collection | DOAJ |
| description | Abstract Background CRISPR/Cas9 technology has garnered increasing attention for its simplicity and precision in genome editing, making it an indispensable tool for gene function research and crop genetic improvement. However, the inefficiency and time-consuming nature of genetic transformation continue to pose substantial challenges to its widespread application in woody plants. Results In this study, we developed a rapid and efficient Agrobacterium-mediated transformation system using petioles as explants for kiwifruit. Positive resistant seedlings were obtained within three months by inoculating on MS medium supplemented with 2.0 mg·L−1 6-benzylaminopurine (6-BA), 0.2 mg·L−1 naphthaleneacetic acid (NAA), and 10 mg·L−1 hygromycin, which was faster than using leaves as explants. Using this system, CRISPR/Cas9-mediated editing of phytoene desaturase (AcPDS) and ζ-carotene desaturase (AcZDS) achieved an editing efficiency of 20%. Transgenic kiwifruit lines with edited AcZDS exhibited a significant reduction in carotenoid content. Conclusions Overall, we established an efficient Agrobacterium-mediated transformation system using petioles as explants, which is applicable for CRISPR/Cas9-mediated gene editing in kiwifruit, thereby facilitating functional gene studies and genetic improvement. |
| format | Article |
| id | doaj-art-95fd5c0e2451435f9fcadafd45e3d7fd |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-95fd5c0e2451435f9fcadafd45e3d7fd2025-08-20T03:04:37ZengBMCBMC Plant Biology1471-22292025-08-0125111310.1186/s12870-025-07112-6CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruitXiaoyan Luo0Ying Dou1Yuxuan Lang2Huamei Zhao3Xinling Liu4Xiaoli Zhang5Yuxing Li6Dong Liang7Hui Xia8College of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityCollege of Horticulture, Sichuan Agricultural UniversityAbstract Background CRISPR/Cas9 technology has garnered increasing attention for its simplicity and precision in genome editing, making it an indispensable tool for gene function research and crop genetic improvement. However, the inefficiency and time-consuming nature of genetic transformation continue to pose substantial challenges to its widespread application in woody plants. Results In this study, we developed a rapid and efficient Agrobacterium-mediated transformation system using petioles as explants for kiwifruit. Positive resistant seedlings were obtained within three months by inoculating on MS medium supplemented with 2.0 mg·L−1 6-benzylaminopurine (6-BA), 0.2 mg·L−1 naphthaleneacetic acid (NAA), and 10 mg·L−1 hygromycin, which was faster than using leaves as explants. Using this system, CRISPR/Cas9-mediated editing of phytoene desaturase (AcPDS) and ζ-carotene desaturase (AcZDS) achieved an editing efficiency of 20%. Transgenic kiwifruit lines with edited AcZDS exhibited a significant reduction in carotenoid content. Conclusions Overall, we established an efficient Agrobacterium-mediated transformation system using petioles as explants, which is applicable for CRISPR/Cas9-mediated gene editing in kiwifruit, thereby facilitating functional gene studies and genetic improvement.https://doi.org/10.1186/s12870-025-07112-6CRISPR/Cas9Genetic transformationKiwifruitPhytoene desaturase (PDS)ζ-carotene desaturase (ZDS) |
| spellingShingle | Xiaoyan Luo Ying Dou Yuxuan Lang Huamei Zhao Xinling Liu Xiaoli Zhang Yuxing Li Dong Liang Hui Xia CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit BMC Plant Biology CRISPR/Cas9 Genetic transformation Kiwifruit Phytoene desaturase (PDS) ζ-carotene desaturase (ZDS) |
| title | CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| title_full | CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| title_fullStr | CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| title_full_unstemmed | CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| title_short | CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| title_sort | crispr cas9 mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit |
| topic | CRISPR/Cas9 Genetic transformation Kiwifruit Phytoene desaturase (PDS) ζ-carotene desaturase (ZDS) |
| url | https://doi.org/10.1186/s12870-025-07112-6 |
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