Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation
Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was...
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2014-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1155/2014/392615 |
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author | Gunashree B. Shivanna Govindarajulu Venkateswaran |
author_facet | Gunashree B. Shivanna Govindarajulu Venkateswaran |
author_sort | Gunashree B. Shivanna |
collection | DOAJ |
description | Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase from Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media. Aspergillus niger CFR 335 and A. ficuum produced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF by A. niger CFR 335 and A.ficuum, respectively, when grown in potato dextrose broth. |
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institution | Kabale University |
issn | 2356-6140 1537-744X |
language | English |
publishDate | 2014-01-01 |
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series | The Scientific World Journal |
spelling | doaj-art-95fb31630f8748e6ae6f1e72686ce3cc2025-02-03T06:00:45ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/392615392615Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State FermentationGunashree B. Shivanna0Govindarajulu Venkateswaran1Department of Food Microbiology, Central Food Technological Research Institute, Mysore, Karnataka-570 020, IndiaDepartment of Food Microbiology, Central Food Technological Research Institute, Mysore, Karnataka-570 020, IndiaFermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase from Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media. Aspergillus niger CFR 335 and A. ficuum produced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF by A. niger CFR 335 and A.ficuum, respectively, when grown in potato dextrose broth.http://dx.doi.org/10.1155/2014/392615 |
spellingShingle | Gunashree B. Shivanna Govindarajulu Venkateswaran Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation The Scientific World Journal |
title | Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation |
title_full | Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation |
title_fullStr | Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation |
title_full_unstemmed | Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation |
title_short | Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation |
title_sort | phytase production by aspergillus niger cfr 335 and aspergillus ficuum sga 01 through submerged and solid state fermentation |
url | http://dx.doi.org/10.1155/2014/392615 |
work_keys_str_mv | AT gunashreebshivanna phytaseproductionbyaspergillusnigercfr335andaspergillusficuumsga01throughsubmergedandsolidstatefermentation AT govindarajuluvenkateswaran phytaseproductionbyaspergillusnigercfr335andaspergillusficuumsga01throughsubmergedandsolidstatefermentation |