HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2019/9365654 |
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| author | Geehyuk Kim Jemberu Taye Kwangmin Yu Sunyoung Park Jungho Kim Sunghyun Kim Dongsup Lee Hye-young Wang Kwang Hwa Park Hyeyoung Lee |
| author_facet | Geehyuk Kim Jemberu Taye Kwangmin Yu Sunyoung Park Jungho Kim Sunghyun Kim Dongsup Lee Hye-young Wang Kwang Hwa Park Hyeyoung Lee |
| author_sort | Geehyuk Kim |
| collection | DOAJ |
| description | After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods. Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted. And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study. However, it takes a long time to collect enough specimens to conduct statistical analysis. Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted. The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing. And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers. |
| format | Article |
| id | doaj-art-95f2106a20604c3caaa42a2d80cad287 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-95f2106a20604c3caaa42a2d80cad2872025-02-03T06:46:04ZengWileyAnalytical Cellular Pathology2210-71772210-71852019-01-01201910.1155/2019/93656549365654HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope SlidesGeehyuk Kim0Jemberu Taye1Kwangmin Yu2Sunyoung Park3Jungho Kim4Sunghyun Kim5Dongsup Lee6Hye-young Wang7Kwang Hwa Park8Hyeyoung Lee9Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of KoreaDepartment of Pathology, Yonsei University Wonju College of Medicine, Wonju, Gangwon Province, Republic of KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of KoreaDepartment of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan, Republic of KoreaDepartment of Clinical Laboratory Science, Hyejeon College, Hongseong, Chungnam Province, Republic of KoreaM&D, Inc., Wonju Eco Environmental Technology Center, Wonju, Gangwon Province, Republic of KoreaDepartment of Pathology, Yonsei University Wonju College of Medicine, Wonju, Gangwon Province, Republic of KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Gangwon Province, Republic of KoreaAfter breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods. Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted. And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study. However, it takes a long time to collect enough specimens to conduct statistical analysis. Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted. The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing. And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers.http://dx.doi.org/10.1155/2019/9365654 |
| spellingShingle | Geehyuk Kim Jemberu Taye Kwangmin Yu Sunyoung Park Jungho Kim Sunghyun Kim Dongsup Lee Hye-young Wang Kwang Hwa Park Hyeyoung Lee HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides Analytical Cellular Pathology |
| title | HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides |
| title_full | HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides |
| title_fullStr | HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides |
| title_full_unstemmed | HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides |
| title_short | HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides |
| title_sort | hpv e6 e7 htert and ki67 mrna rt qpcr assay for detecting high grade cervical lesion with microscope slides |
| url | http://dx.doi.org/10.1155/2019/9365654 |
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