Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury
Objective(s): Anemoside B4 (AB4) is a multifunctional compound with anti-inflammatory, anti-apoptotic, antioxidant, antiviral, and autophagy-enhancing effects. However, the role of AB4 in cerebral ischemia/reperfusion injury (CIRI) remains obscure. This experiment aims to investigate the pharmacolog...
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_24885_901867db25a318b489c1ccc55e529539.pdf |
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| author | Fei Huizhi Xiaohuan Huang |
| author_facet | Fei Huizhi Xiaohuan Huang |
| author_sort | Fei Huizhi |
| collection | DOAJ |
| description | Objective(s): Anemoside B4 (AB4) is a multifunctional compound with anti-inflammatory, anti-apoptotic, antioxidant, antiviral, and autophagy-enhancing effects. However, the role of AB4 in cerebral ischemia/reperfusion injury (CIRI) remains obscure. This experiment aims to investigate the pharmacological effects of AB4 in CIRI.Materials and Methods: In vivo, eighty male SD rats were randomly divided into five groups: sham, MCAO/R, LD group (2.5 mg/kg), MD group (5 mg/kg), and HD group (10 mg/kg). The rats in sham and MCAO/R groups were given equal volumes of normal saline. In vitro, PC12 cells were divided into five groups: normal, OGD/R, OGD/R+AB4 (50 μM), OGD/R+AB4 (100 μM), and OGD/R+AB4 (200 μM). The cells were treated with hypoxia and hypoglycemia for 1.5 hr and reoxygenation for 24 hr.Results: In vivo, TTC and neurological scoring tests indicated that AB4 favors promoting the recovery of the brain. The histopathologic study of the brain tissues revealed that AB4 inhibited the damage of neuron cells. The TUNEL assay found that AB4 could improve cell apoptosis and prevent the brain from injury. In vitro, the data showed that AB4 inhibited cell damage and prevented PC12 cells from OGD/R injury, reduced IL-1β content, and increased the IL-10 level. AB4 could inhibit apoptosis of PC12 cells, down-regulate Caspase 12 and BAX expression, and up-regulate Bcl-2 expression.Conclusion: AB4 played a protective role in CIRI and could be a promising active ingredient against ischemia stroke. |
| format | Article |
| id | doaj-art-95efa28898c244c3823309ecc929fcfa |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-95efa28898c244c3823309ecc929fcfa2025-08-20T02:48:06ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-01-01281495510.22038/ijbms.2024.78569.1699124885Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injuryFei Huizhi0Xiaohuan Huang1Medical college, Jiujiang University, Jiujiang, Jiangxi Province 332200, ChinaDepartment of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing 404100, ChinaObjective(s): Anemoside B4 (AB4) is a multifunctional compound with anti-inflammatory, anti-apoptotic, antioxidant, antiviral, and autophagy-enhancing effects. However, the role of AB4 in cerebral ischemia/reperfusion injury (CIRI) remains obscure. This experiment aims to investigate the pharmacological effects of AB4 in CIRI.Materials and Methods: In vivo, eighty male SD rats were randomly divided into five groups: sham, MCAO/R, LD group (2.5 mg/kg), MD group (5 mg/kg), and HD group (10 mg/kg). The rats in sham and MCAO/R groups were given equal volumes of normal saline. In vitro, PC12 cells were divided into five groups: normal, OGD/R, OGD/R+AB4 (50 μM), OGD/R+AB4 (100 μM), and OGD/R+AB4 (200 μM). The cells were treated with hypoxia and hypoglycemia for 1.5 hr and reoxygenation for 24 hr.Results: In vivo, TTC and neurological scoring tests indicated that AB4 favors promoting the recovery of the brain. The histopathologic study of the brain tissues revealed that AB4 inhibited the damage of neuron cells. The TUNEL assay found that AB4 could improve cell apoptosis and prevent the brain from injury. In vitro, the data showed that AB4 inhibited cell damage and prevented PC12 cells from OGD/R injury, reduced IL-1β content, and increased the IL-10 level. AB4 could inhibit apoptosis of PC12 cells, down-regulate Caspase 12 and BAX expression, and up-regulate Bcl-2 expression.Conclusion: AB4 played a protective role in CIRI and could be a promising active ingredient against ischemia stroke.https://ijbms.mums.ac.ir/article_24885_901867db25a318b489c1ccc55e529539.pdfanemoside b4apoptosiscerebral ischemia/reperfusionmiddle cerebral artery occlusion/reperfusionoxygen-glucose-deprivation/re-oxygenation |
| spellingShingle | Fei Huizhi Xiaohuan Huang Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury Iranian Journal of Basic Medical Sciences anemoside b4 apoptosis cerebral ischemia/reperfusion middle cerebral artery occlusion/reperfusion oxygen-glucose-deprivation/re-oxygenation |
| title | Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury |
| title_full | Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury |
| title_fullStr | Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury |
| title_full_unstemmed | Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury |
| title_short | Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury |
| title_sort | effect of anemoside b4 on ameliorating cerebral ischemic reperfusion injury |
| topic | anemoside b4 apoptosis cerebral ischemia/reperfusion middle cerebral artery occlusion/reperfusion oxygen-glucose-deprivation/re-oxygenation |
| url | https://ijbms.mums.ac.ir/article_24885_901867db25a318b489c1ccc55e529539.pdf |
| work_keys_str_mv | AT feihuizhi effectofanemosideb4onamelioratingcerebralischemicreperfusioninjury AT xiaohuanhuang effectofanemosideb4onamelioratingcerebralischemicreperfusioninjury |