Upregulation of miR-152 inhibits proliferation and migration of human vascular smooth muscle cells induced by ox-LDL
Objective To investigate the roles of miR-152 and Rho-associated coiled-coil kinase 1 (ROCK1) in vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (ox-LDL). Methods Human vascular smooth muscle cells CRL-1999 were intervened with ox-LDL to establish an in vitro ath...
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| Main Author: | |
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| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-4-486.pdf |
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| Summary: | Objective To investigate the roles of miR-152 and Rho-associated coiled-coil kinase 1 (ROCK1) in vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (ox-LDL). Methods Human vascular smooth muscle cells CRL-1999 were intervened with ox-LDL to establish an in vitro atherosclerosis model. miR-152 mimic and ROCK1 siRNA were transfected into corresponding cells. RT-qPCR was used to detect the expression of miR-152 and ROCK1 mRNA. The CCK-8 method was used to assess cell proliferation activity, and the Transwell assay was used to test cell migration ability. A luciferase reporter gene assay was conducted to determine the targeting relationship between miR-152 and ROCK1. Results CRL-1999 cells treated with 100 μg/mL ox-LDL for 48 hours showed the maximum proliferation rate. Compared with the untreated control group, the relative expression of miR-152 in the ox-LDL-induced group was significantly decreased (P＜0.05). Transfection of miR-152 mimic significantly reduced the proliferation and migration of CRL-1999 cells (P＜0.05). The luciferase reporter gene assay indicated that miR-152 targets ROCK1, and expression of ROCK1 mRNA and protein in miR-152 mimic group was reduced(P＜0.05). Transfection of ROCK1 siRNA also significantly decreased the proliferation and migration of CRL-1999 cells(P＜0.05). Conclusions miR-152 inhibits the ox-LDL induced proliferation and migration of CRL-1999 cells by downregulating ROCK1 expression, which may be a potential therapeutic target for atherosclerosis. |
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| ISSN: | 1001-6325 |