Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses

The mixed fermentation of non-Saccharomyces yeasts with Saccharomyces cerevisiae (S. cerevisiae) is regarded as a promising strategy to improve the quality of fruit wine. In this study, we investigated the effects of sequential inoculation fermentation involving four non-Saccharomyces yeast species...

Full description

Saved in:
Bibliographic Details
Main Authors: Xin-ying Xu, Jun-bo Wei, Ruo-ting Hou, Mei-qing Li, Yu Wang, Xue-Ling Gao, Jin Liang, Hai-wei Zhang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225001982
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850220683176443904
author Xin-ying Xu
Jun-bo Wei
Ruo-ting Hou
Mei-qing Li
Yu Wang
Xue-Ling Gao
Jin Liang
Hai-wei Zhang
author_facet Xin-ying Xu
Jun-bo Wei
Ruo-ting Hou
Mei-qing Li
Yu Wang
Xue-Ling Gao
Jin Liang
Hai-wei Zhang
author_sort Xin-ying Xu
collection DOAJ
description The mixed fermentation of non-Saccharomyces yeasts with Saccharomyces cerevisiae (S. cerevisiae) is regarded as a promising strategy to improve the quality of fruit wine. In this study, we investigated the effects of sequential inoculation fermentation involving four non-Saccharomyces yeast species (Tourlaspora delbrueckii (T.d.), Metschnikowia pulcherrima (M.p.), Schizosaccharomyces pombe (S.p.) and Issatchenkia terricola (I.t.)) and an S. cerevisiae strain on the physiochemical properties, volatile compounds and sensory profiling of strawberry wine. The sequential fermentations exhibited a significant reduction in ethanol and organic acids content, while free amino acids content increased by 0.28–3.74 times, particularly in the M.p.+S.c. group. The GC-IMS identified 68 volatile compounds, among which isoamyl acetate, ethyl hexanoate, and isobutanol were found to be the predominant volatiles of strawberry wine. Samples more frequently characterized as “strawberry”, “honey” and “preserved fruit” in Check-all-that-apply analysis were preferred by the panelists (n = 93). The I.t.+S.c. elicited emotional responses of “fascinated” and “surprised” leading to a higher liking rating. Furthermore, the PLSR analysis demonstrated a significant contribution of esters and higher alcohols, such as ethyl 3-methylbutanoate-D, ethyl propanoate, along with isoamyl acetate-D, to the fruity and sweet notes. The present study provided useful information into the mixed inoculation fermentation strategy, which involves combining appropriate non-Saccharomyces yeasts with S. cerevisiae, for developing distinct styles and enhancing the oenological quality of strawberry wine.
format Article
id doaj-art-95c5ef4950cc49e092cde6af19ec96e4
institution OA Journals
issn 2772-5022
language English
publishDate 2025-06-01
publisher Elsevier
record_format Article
series Applied Food Research
spelling doaj-art-95c5ef4950cc49e092cde6af19ec96e42025-08-20T02:06:58ZengElsevierApplied Food Research2772-50222025-06-015110089010.1016/j.afres.2025.100890Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analysesXin-ying Xu0Jun-bo Wei1Ruo-ting Hou2Mei-qing Li3Yu Wang4Xue-Ling Gao5Jin Liang6Hai-wei Zhang7Joint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaJoint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaCorresponding author.; Joint Research Center for Food Nutrition and Health of IHM, Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaThe mixed fermentation of non-Saccharomyces yeasts with Saccharomyces cerevisiae (S. cerevisiae) is regarded as a promising strategy to improve the quality of fruit wine. In this study, we investigated the effects of sequential inoculation fermentation involving four non-Saccharomyces yeast species (Tourlaspora delbrueckii (T.d.), Metschnikowia pulcherrima (M.p.), Schizosaccharomyces pombe (S.p.) and Issatchenkia terricola (I.t.)) and an S. cerevisiae strain on the physiochemical properties, volatile compounds and sensory profiling of strawberry wine. The sequential fermentations exhibited a significant reduction in ethanol and organic acids content, while free amino acids content increased by 0.28–3.74 times, particularly in the M.p.+S.c. group. The GC-IMS identified 68 volatile compounds, among which isoamyl acetate, ethyl hexanoate, and isobutanol were found to be the predominant volatiles of strawberry wine. Samples more frequently characterized as “strawberry”, “honey” and “preserved fruit” in Check-all-that-apply analysis were preferred by the panelists (n = 93). The I.t.+S.c. elicited emotional responses of “fascinated” and “surprised” leading to a higher liking rating. Furthermore, the PLSR analysis demonstrated a significant contribution of esters and higher alcohols, such as ethyl 3-methylbutanoate-D, ethyl propanoate, along with isoamyl acetate-D, to the fruity and sweet notes. The present study provided useful information into the mixed inoculation fermentation strategy, which involves combining appropriate non-Saccharomyces yeasts with S. cerevisiae, for developing distinct styles and enhancing the oenological quality of strawberry wine.http://www.sciencedirect.com/science/article/pii/S2772502225001982Strawberry wineNon-Saccharomyces yeastSequential fermentationAroma profilesGC-IMSCATA
spellingShingle Xin-ying Xu
Jun-bo Wei
Ruo-ting Hou
Mei-qing Li
Yu Wang
Xue-Ling Gao
Jin Liang
Hai-wei Zhang
Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
Applied Food Research
Strawberry wine
Non-Saccharomyces yeast
Sequential fermentation
Aroma profiles
GC-IMS
CATA
title Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
title_full Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
title_fullStr Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
title_full_unstemmed Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
title_short Sequential inoculation of non-Saccharomyces yeasts and Saccharomyces cerevisiae modulates strawberry wine quality: Physicochemical, volatile and sensory analyses
title_sort sequential inoculation of non saccharomyces yeasts and saccharomyces cerevisiae modulates strawberry wine quality physicochemical volatile and sensory analyses
topic Strawberry wine
Non-Saccharomyces yeast
Sequential fermentation
Aroma profiles
GC-IMS
CATA
url http://www.sciencedirect.com/science/article/pii/S2772502225001982
work_keys_str_mv AT xinyingxu sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT junbowei sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT ruotinghou sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT meiqingli sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT yuwang sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT xuelinggao sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT jinliang sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses
AT haiweizhang sequentialinoculationofnonsaccharomycesyeastsandsaccharomycescerevisiaemodulatesstrawberrywinequalityphysicochemicalvolatileandsensoryanalyses