Using Beads as a Focus Fiduciary to Aid Software-Based Autofocus Accuracy in Microscopy

Brightfield microscopy is an ideal application for studying live cell systems in a minimally invasive manner. This is advantageous in long-term experiments to study dynamic cellular processes such as stress response. Depending on the sample type and preparation, the inherent qualities of brightfield...

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Bibliographic Details
Main Authors: Isabel Gibson, Elizabeth Osterlund, Ray Truant
Format: Article
Language:English
Published: Bio-protocol LLC 2025-07-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5388&type=0
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