Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model
<i>Background and Objectives</i>: Wound healing is a complex process involving cellular, anatomical, and functional repair, often hindered in chronic wounds associated with diseases like diabetes and vascular disorders. This study investigated the efficacy of conventional and regenerativ...
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MDPI AG
2024-11-01
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| author | Rossella Elia Michele Maruccia Pietro Giovanni Di Summa Rodrigo Trisciuzzi Giuditta Lovero Gerardo Cazzato Luca Lacitignola Francesco Staffieri Alberto Maria Crovace |
| author_facet | Rossella Elia Michele Maruccia Pietro Giovanni Di Summa Rodrigo Trisciuzzi Giuditta Lovero Gerardo Cazzato Luca Lacitignola Francesco Staffieri Alberto Maria Crovace |
| author_sort | Rossella Elia |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Wound healing is a complex process involving cellular, anatomical, and functional repair, often hindered in chronic wounds associated with diseases like diabetes and vascular disorders. This study investigated the efficacy of conventional and regenerative wound healing approaches in a sheep surgical wound model. <i>Materials and Methods</i>: Six female Bergamasca sheep underwent five full-thickness skin lesions treated with various methods: sterile gauze (control), chlorhexidine, sodium hypochlorite, micronized dermis system application, and dermal matrix. Wound healing progression was monitored over 42 days through wound dimension measurements, exudate analysis, and histopathological evaluations. <i>Results</i>: The results indicated that all wounds healed completely by day 42, with significant reductions in wound size and exudate over time. Notably, Micronized dermis system application and dermal matrix treatments showed a faster evolution in exudate characteristics and improved collagen reorganization compared to other treatments. Histological analysis revealed earlier neovascularization and better reconstitution of hair follicles in these groups. Despite the lack of significant differences in healing time, both regenerative approaches enhanced wound healing phases, contributing to exudate control, angiogenesis promotion, and reduced scar formation. <i>Conclusions</i>: The findings suggest that while micronized dermis system application and dermal matrix do not accelerate acute wound healing compared to conventional methods, they offer potential benefits in managing exudate and improving tissue regeneration, warranting further investigation in chronic wound scenarios. |
| format | Article |
| id | doaj-art-959fb2bfdab1411397b46f7f950ceff6 |
| institution | OA Journals |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj-art-959fb2bfdab1411397b46f7f950ceff62025-08-20T02:04:54ZengMDPI AGMedicina1010-660X1648-91442024-11-016011183610.3390/medicina60111836Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep ModelRossella Elia0Michele Maruccia1Pietro Giovanni Di Summa2Rodrigo Trisciuzzi3Giuditta Lovero4Gerardo Cazzato5Luca Lacitignola6Francesco Staffieri7Alberto Maria Crovace8Unit of Plastic and Reconstructive Surgery, Department of Precision and Regenerative Medicine and Jonic Area, University of Bari, 11, Piazza Giulio Cesare, 70124 Bari, ItalyUnit of Plastic and Reconstructive Surgery, Department of Precision and Regenerative Medicine and Jonic Area, University of Bari, 11, Piazza Giulio Cesare, 70124 Bari, ItalyDepartment of Plastic and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV), University of Lausanne (UNIL), Rue du Bugnon 46, 1011 Lausanne, SwitzerlandDepartment of Precision-Regenerative Medicine and Jonic Area (DiMePRe-J), Section of Veterinary Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyUnit of Plastic and Reconstructive Surgery, Department of Precision and Regenerative Medicine and Jonic Area, University of Bari, 11, Piazza Giulio Cesare, 70124 Bari, ItalySection of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, 70124 Bari, ItalyDepartment of Precision-Regenerative Medicine and Jonic Area (DiMePRe-J), Section of Veterinary Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Precision-Regenerative Medicine and Jonic Area (DiMePRe-J), Section of Veterinary Medicine, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy<i>Background and Objectives</i>: Wound healing is a complex process involving cellular, anatomical, and functional repair, often hindered in chronic wounds associated with diseases like diabetes and vascular disorders. This study investigated the efficacy of conventional and regenerative wound healing approaches in a sheep surgical wound model. <i>Materials and Methods</i>: Six female Bergamasca sheep underwent five full-thickness skin lesions treated with various methods: sterile gauze (control), chlorhexidine, sodium hypochlorite, micronized dermis system application, and dermal matrix. Wound healing progression was monitored over 42 days through wound dimension measurements, exudate analysis, and histopathological evaluations. <i>Results</i>: The results indicated that all wounds healed completely by day 42, with significant reductions in wound size and exudate over time. Notably, Micronized dermis system application and dermal matrix treatments showed a faster evolution in exudate characteristics and improved collagen reorganization compared to other treatments. Histological analysis revealed earlier neovascularization and better reconstitution of hair follicles in these groups. Despite the lack of significant differences in healing time, both regenerative approaches enhanced wound healing phases, contributing to exudate control, angiogenesis promotion, and reduced scar formation. <i>Conclusions</i>: The findings suggest that while micronized dermis system application and dermal matrix do not accelerate acute wound healing compared to conventional methods, they offer potential benefits in managing exudate and improving tissue regeneration, warranting further investigation in chronic wound scenarios.https://www.mdpi.com/1648-9144/60/11/1836woundwound bed preparationdermal matrixmicrograft |
| spellingShingle | Rossella Elia Michele Maruccia Pietro Giovanni Di Summa Rodrigo Trisciuzzi Giuditta Lovero Gerardo Cazzato Luca Lacitignola Francesco Staffieri Alberto Maria Crovace Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model Medicina wound wound bed preparation dermal matrix micrograft |
| title | Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model |
| title_full | Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model |
| title_fullStr | Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model |
| title_full_unstemmed | Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model |
| title_short | Conventional Versus Regenerative Methods for Wound Healing: A Comparative Experimental Study on a Sheep Model |
| title_sort | conventional versus regenerative methods for wound healing a comparative experimental study on a sheep model |
| topic | wound wound bed preparation dermal matrix micrograft |
| url | https://www.mdpi.com/1648-9144/60/11/1836 |
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