Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy
Background. Ultraviolet irradiation of the skin can activate the cytoplasmic aryl hydrocarbon receptor AhR, which, in combination with its nuclear translocator ARNT binds to the promoter of the FLG gene encoding filaggrin, a protective skin barrier protein. This suggests that the therapeutic effect...
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State Scientific Center of Dermatovenereology and Cosmetology
2025-03-01
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| Series: | Vestnik Dermatologii i Venerologii |
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| Online Access: | https://vestnikdv.ru/jour/article/viewFile/16839/pdf_1 |
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| author | Irina V. Kozlova Vadim V. Chikin Pavel V. Gorodnichev Kseniya M. Lagun Nikita Y. Nosov |
| author_facet | Irina V. Kozlova Vadim V. Chikin Pavel V. Gorodnichev Kseniya M. Lagun Nikita Y. Nosov |
| author_sort | Irina V. Kozlova |
| collection | DOAJ |
| description | Background. Ultraviolet irradiation of the skin can activate the cytoplasmic aryl hydrocarbon receptor AhR, which, in combination with its nuclear translocator ARNT binds to the promoter of the FLG gene encoding filaggrin, a protective skin barrier protein. This suggests that the therapeutic effect of phototherapy may be due not only to the immunosuppressive effect, but also to stimulation of filaggrin production.
Aims. To evaluate the effect of ultraviolet irradiation used for various phototherapy methods on the expression levels of the FLG, AHR and ARNT genes in the skin of patients with atopic dermatitis.
Methods. The expression levels of the FLG, AHR and ARNT genes were determined using real-time PCR. To define the severity of the disease, the SCORAD index was calculated.
Results. 76 patients with atopic dermatitis were included in the study. 37 patients underwent narrow-band phototherapy; 39 — UFA1 therapy. Both methods of phototherapy were effective. After narrow-band phototherapy, the level of expression of the FLG, AHR and ARNT genes significantly increased. After UFA1 therapy, the expression of the AHR and ARNT genes increased, and no changes in the expression of the FLG gene were observed.
Conclusions. Data have been obtained indicating that the therapeutic effect of narrow-band phototherapy in atopic dermatitis may be due to stimulation of the expression of the FLG gene in the skin. The revealed changes in the expression of the AHR and ARNT genes indicate the possible involvement of their protein products AhR and ARNT in the pathogenesis of the disease. |
| format | Article |
| id | doaj-art-958697e6b9eb4f77a90eb9a9a4471106 |
| institution | Kabale University |
| issn | 0042-4609 2313-6294 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | State Scientific Center of Dermatovenereology and Cosmetology |
| record_format | Article |
| series | Vestnik Dermatologii i Venerologii |
| spelling | doaj-art-958697e6b9eb4f77a90eb9a9a44711062025-08-20T03:47:41ZengState Scientific Center of Dermatovenereology and CosmetologyVestnik Dermatologii i Venerologii0042-46092313-62942025-03-011011596810.25208/vdv168391325Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapyIrina V. Kozlova0https://orcid.org/0000-0002-6328-363XVadim V. Chikin1https://orcid.org/0000-0002-9688-2727Pavel V. Gorodnichev2https://orcid.org/0000-0001-5989-7156Kseniya M. Lagun3https://orcid.org/0009-0004-9700-2455Nikita Y. Nosov4https://orcid.org/0000-0002-3967-8359State Research Center of Dermatovenereology and CosmetologyState Research Center of Dermatovenereology and CosmetologyState Research Center of Dermatovenereology and CosmetologyState Research Center of Dermatovenereology and CosmetologyState Research Center of Dermatovenereology and CosmetologyBackground. Ultraviolet irradiation of the skin can activate the cytoplasmic aryl hydrocarbon receptor AhR, which, in combination with its nuclear translocator ARNT binds to the promoter of the FLG gene encoding filaggrin, a protective skin barrier protein. This suggests that the therapeutic effect of phototherapy may be due not only to the immunosuppressive effect, but also to stimulation of filaggrin production. Aims. To evaluate the effect of ultraviolet irradiation used for various phototherapy methods on the expression levels of the FLG, AHR and ARNT genes in the skin of patients with atopic dermatitis. Methods. The expression levels of the FLG, AHR and ARNT genes were determined using real-time PCR. To define the severity of the disease, the SCORAD index was calculated. Results. 76 patients with atopic dermatitis were included in the study. 37 patients underwent narrow-band phototherapy; 39 — UFA1 therapy. Both methods of phototherapy were effective. After narrow-band phototherapy, the level of expression of the FLG, AHR and ARNT genes significantly increased. After UFA1 therapy, the expression of the AHR and ARNT genes increased, and no changes in the expression of the FLG gene were observed. Conclusions. Data have been obtained indicating that the therapeutic effect of narrow-band phototherapy in atopic dermatitis may be due to stimulation of the expression of the FLG gene in the skin. The revealed changes in the expression of the AHR and ARNT genes indicate the possible involvement of their protein products AhR and ARNT in the pathogenesis of the disease.https://vestnikdv.ru/jour/article/viewFile/16839/pdf_1atopic dermatitisnarrow-band ultraviolet phototherapyaryl hydrocarbon receptorfilaggringene expression |
| spellingShingle | Irina V. Kozlova Vadim V. Chikin Pavel V. Gorodnichev Kseniya M. Lagun Nikita Y. Nosov Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy Vestnik Dermatologii i Venerologii atopic dermatitis narrow-band ultraviolet phototherapy aryl hydrocarbon receptor filaggrin gene expression |
| title | Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy |
| title_full | Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy |
| title_fullStr | Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy |
| title_full_unstemmed | Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy |
| title_short | Evaluation of the expression of <i>FLG, AHR</i> and <i>ARNT</i> genes in the skin of patients with atopic dermatitis after phototherapy |
| title_sort | evaluation of the expression of i flg ahr i and i arnt i genes in the skin of patients with atopic dermatitis after phototherapy |
| topic | atopic dermatitis narrow-band ultraviolet phototherapy aryl hydrocarbon receptor filaggrin gene expression |
| url | https://vestnikdv.ru/jour/article/viewFile/16839/pdf_1 |
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