Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma
Introduction: Several micro ribonucleic acids (miRNAs) are deregulated in hepatocellular carcinoma (HCC). Others are linked to clinical pathological features of HCC. The goal of this study was to investigate whether miRNA-21 and miRNA-215 gene expression could be used as a non-invasive diagnostic t...
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The Journal of Infection in Developing Countries
2021-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12230 |
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| author | Zeinab Sayed Abdelkhalek Mohamed Shehata Abdalla Mona Mohamed Fathy Tamer Mahmoud Elbaz Ashraf Omar Abdelaziz Mohamed Mahmoud Nabeel Hend Ibrahim Shousha Amgad Hamed Kamel Mai Hamed Kamel |
| author_facet | Zeinab Sayed Abdelkhalek Mohamed Shehata Abdalla Mona Mohamed Fathy Tamer Mahmoud Elbaz Ashraf Omar Abdelaziz Mohamed Mahmoud Nabeel Hend Ibrahim Shousha Amgad Hamed Kamel Mai Hamed Kamel |
| author_sort | Zeinab Sayed Abdelkhalek |
| collection | DOAJ |
| description |
Introduction: Several micro ribonucleic acids (miRNAs) are deregulated in hepatocellular carcinoma (HCC). Others are linked to clinical pathological features of HCC. The goal of this study was to investigate whether miRNA-21 and miRNA-215 gene expression could be used as a non-invasive diagnostic tool to diagnose HCC.
Methodology: The gene expression of mature miRNA -21 and miRNA -215 in serum was analysed retrospectively using singleplex TaqMan two-step stem-loop quantitative real-time reverse-transcription PCR in 40 patients with HCC, 40 with chronic hepatitis C virus (HCV) with cirrhosis and 40 apparently healthy controls.
Results: Expression of miRNA -21 was significantly more down regulated in patients with HCC than in those with non-cirrhotic HCV (P = 0.007; odds ratio = 5; 95% confidence interval 1.6–15.4). The receiver operating curve analysis of the ability of miRNA-21 expression to discriminate between HCC and non-cirrhotic HCV revealed an area under the curve of 0.712 with 70% sensitivity and 68% specificity at a cut-off of ≤ 1.4468. Thus, the expression level of miRNA -21 could discriminate HCC from non-cirrhotic HCV. Significant positive correlation was observed between expression levels of microRNA-21 and miRNA -215 (r = 0.783, p < 0.001), but no association was observed between expression level of miR-215 and HCC or chronic HCV (p = 0.474).
Conclusions: MiRNA-21 may be a useful, non-invasive tool for diagnosing HCC. Non-cirrhotic HCV patients have five times the risk of developing HCC when the miRNA -21 level ≤ 1.4468.
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| format | Article |
| id | doaj-art-957c35d0b747404194b05b17ca5262d6 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-957c35d0b747404194b05b17ca5262d62025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-07-01150710.3855/jidc.12230Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinomaZeinab Sayed Abdelkhalek0Mohamed Shehata Abdalla 1Mona Mohamed Fathy2Tamer Mahmoud Elbaz3Ashraf Omar Abdelaziz4Mohamed Mahmoud Nabeel5Hend Ibrahim Shousha6Amgad Hamed Kamel7Mai Hamed Kamel8Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, EgyptClinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, EgyptClinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, EgyptEndemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, EgyptEndemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, EgyptEndemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, EgyptEndemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Tropical medicine, Faculty of Medicine, Ain Shams University, Cairo, EgyptClinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt Introduction: Several micro ribonucleic acids (miRNAs) are deregulated in hepatocellular carcinoma (HCC). Others are linked to clinical pathological features of HCC. The goal of this study was to investigate whether miRNA-21 and miRNA-215 gene expression could be used as a non-invasive diagnostic tool to diagnose HCC. Methodology: The gene expression of mature miRNA -21 and miRNA -215 in serum was analysed retrospectively using singleplex TaqMan two-step stem-loop quantitative real-time reverse-transcription PCR in 40 patients with HCC, 40 with chronic hepatitis C virus (HCV) with cirrhosis and 40 apparently healthy controls. Results: Expression of miRNA -21 was significantly more down regulated in patients with HCC than in those with non-cirrhotic HCV (P = 0.007; odds ratio = 5; 95% confidence interval 1.6–15.4). The receiver operating curve analysis of the ability of miRNA-21 expression to discriminate between HCC and non-cirrhotic HCV revealed an area under the curve of 0.712 with 70% sensitivity and 68% specificity at a cut-off of ≤ 1.4468. Thus, the expression level of miRNA -21 could discriminate HCC from non-cirrhotic HCV. Significant positive correlation was observed between expression levels of microRNA-21 and miRNA -215 (r = 0.783, p < 0.001), but no association was observed between expression level of miR-215 and HCC or chronic HCV (p = 0.474). Conclusions: MiRNA-21 may be a useful, non-invasive tool for diagnosing HCC. Non-cirrhotic HCV patients have five times the risk of developing HCC when the miRNA -21 level ≤ 1.4468. https://jidc.org/index.php/journal/article/view/12230Hepatocellular CarcinomadiagnosismicroRNAsTaqMan quantitative real-time reverse-transcription PCR |
| spellingShingle | Zeinab Sayed Abdelkhalek Mohamed Shehata Abdalla Mona Mohamed Fathy Tamer Mahmoud Elbaz Ashraf Omar Abdelaziz Mohamed Mahmoud Nabeel Hend Ibrahim Shousha Amgad Hamed Kamel Mai Hamed Kamel Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma Journal of Infection in Developing Countries Hepatocellular Carcinoma diagnosis microRNAs TaqMan quantitative real-time reverse-transcription PCR |
| title | Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma |
| title_full | Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma |
| title_fullStr | Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma |
| title_full_unstemmed | Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma |
| title_short | Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma |
| title_sort | role of circulating microrna 21 and microrna 215 in the diagnosis of hepatitis c related hepatocellular carcinoma |
| topic | Hepatocellular Carcinoma diagnosis microRNAs TaqMan quantitative real-time reverse-transcription PCR |
| url | https://jidc.org/index.php/journal/article/view/12230 |
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