Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation
Release of extracellular DNA (eDNA) was observed during in vitro growth of a clinical strain of Acinetobacter baumannii. Membrane vesicles (MV) of varying diameter (20–200 nm) containing DNA were found to be released by transmission electron microscopy (TEM) and atomic force microscopy (AFM). An ass...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/973436 |
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| author | Praveen K. Sahu Pavithra S. Iyer Amrita M. Oak Karishma R. Pardesi Balu A. Chopade |
| author_facet | Praveen K. Sahu Pavithra S. Iyer Amrita M. Oak Karishma R. Pardesi Balu A. Chopade |
| author_sort | Praveen K. Sahu |
| collection | DOAJ |
| description | Release of extracellular DNA (eDNA) was observed during in vitro growth of a clinical strain of Acinetobacter baumannii. Membrane vesicles (MV) of varying diameter (20–200 nm) containing DNA were found to be released by transmission electron microscopy (TEM) and atomic force microscopy (AFM). An assessment of the characteristics of the eDNA with respect to size, digestion pattern by DNase I/restriction enzymes, and PCR-sequencing, indicates a high similarity with genomic DNA. Role of eDNA in static biofilm formed on polystyrene surface was evaluated by biofilm augmentation assay using eDNA available in different preparations, for example, whole cell lysate, cell-free supernatant, MV suspension, and purified eDNA. Biofilm augmentation was seen up to 224.64%, whereas biofilm inhibition was 59.41% after DNase I treatment: confirming that eDNA facilitates biofilm formation in A. baumannii. This is the first paper elucidating the characteristics and role of eDNA in A. baumannii biofilm, which may provide new insights into its pathogenesis. |
| format | Article |
| id | doaj-art-957677459b7742d0960beb7c3a788e0f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-957677459b7742d0960beb7c3a788e0f2025-02-03T06:14:00ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/973436973436Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm FormationPraveen K. Sahu0Pavithra S. Iyer1Amrita M. Oak2Karishma R. Pardesi3Balu A. Chopade4Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, IndiaInstitute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, IndiaInstitute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, IndiaDepartment of Microbiology, University of Pune, Pune 411007, IndiaInstitute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, IndiaRelease of extracellular DNA (eDNA) was observed during in vitro growth of a clinical strain of Acinetobacter baumannii. Membrane vesicles (MV) of varying diameter (20–200 nm) containing DNA were found to be released by transmission electron microscopy (TEM) and atomic force microscopy (AFM). An assessment of the characteristics of the eDNA with respect to size, digestion pattern by DNase I/restriction enzymes, and PCR-sequencing, indicates a high similarity with genomic DNA. Role of eDNA in static biofilm formed on polystyrene surface was evaluated by biofilm augmentation assay using eDNA available in different preparations, for example, whole cell lysate, cell-free supernatant, MV suspension, and purified eDNA. Biofilm augmentation was seen up to 224.64%, whereas biofilm inhibition was 59.41% after DNase I treatment: confirming that eDNA facilitates biofilm formation in A. baumannii. This is the first paper elucidating the characteristics and role of eDNA in A. baumannii biofilm, which may provide new insights into its pathogenesis.http://dx.doi.org/10.1100/2012/973436 |
| spellingShingle | Praveen K. Sahu Pavithra S. Iyer Amrita M. Oak Karishma R. Pardesi Balu A. Chopade Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation The Scientific World Journal |
| title | Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation |
| title_full | Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation |
| title_fullStr | Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation |
| title_full_unstemmed | Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation |
| title_short | Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation |
| title_sort | characterization of edna from the clinical strain acinetobacter baumannii aiims 7 and its role in biofilm formation |
| url | http://dx.doi.org/10.1100/2012/973436 |
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