Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’
[Objective] The study aims to explore the function of SAT4 (serine acetyltransferase 4), a member of the peach serine acetyltransferase family, in adventitious root formation, and provide potential target genes for future breeding to improve rooting in peach. [Methods] Using the transcriptome of a...
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Science Press
2025-01-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240400?st=article_issue |
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| author | HAO Lanlan WANG Hong ZHANG Fan ZHANG Xuebing YANG Yang |
| author_facet | HAO Lanlan WANG Hong ZHANG Fan ZHANG Xuebing YANG Yang |
| author_sort | HAO Lanlan |
| collection | DOAJ |
| description | [Objective] The study aims to explore the function of SAT4 (serine acetyltransferase 4), a
member of the peach serine acetyltransferase family, in adventitious root formation, and provide potential
target genes for future breeding to improve rooting in peach. [Methods] Using the transcriptome of adventitious
roots, the PpSAT4 gene was screened and transformed into Prunus domestica and Arabidopsis
thaliana to explore the function of PpSAT4 in adventitious root formation. [Results] (1) Peach PpSAT4
gene with a sequence of 864 bp was cloned, encoding a protein with a molecular weight of 30.49 kD and an
isoelectric point of 6.70. Phylogenetic analysis showed that PpSAT4 had the closest relationship with European
sweet cherries and almonds. Analysis of cis-acting elements showed the presence of response elements
such as light, auxin, and gibberellin in the PpSAT4 sequence. (2) The overexpression vector Pp-
SAT4-CAM-FLAG was constructed and transformed P . domestica and A . thaliana, and the transgenic
lines were obtained. (3) qRT-PCR analysis showed that the expression level of PpSAT4 in the transgenic
lines was higher than that of the wild-type. Overexpression of PpSAT4 increased various indices and POD
and SOD activities in the plum root system. In addition, overexpression of this gene in A . thaliana increased
root length and enzyme activity, suggesting a similar function in A . thaliana. [Conclusion] Pp-
SAT4 plays a crucial role in facilitating the formation of adventitious roots. |
| format | Article |
| id | doaj-art-9570320f90494e1e9751db82195496cb |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-9570320f90494e1e9751db82195496cb2025-02-11T13:42:06ZzhoScience PressXibei zhiwu xuebao1000-40252025-01-014510001001010.7606/j.issn.1000-4025.20240400Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’HAO Lanlan0WANG Hong1ZHANG Fan2ZHANG Xuebing3YANG Yang4Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaInstitute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaInstitute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, ChinaInstitute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, ChinaInstitute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China[Objective] The study aims to explore the function of SAT4 (serine acetyltransferase 4), a member of the peach serine acetyltransferase family, in adventitious root formation, and provide potential target genes for future breeding to improve rooting in peach. [Methods] Using the transcriptome of adventitious roots, the PpSAT4 gene was screened and transformed into Prunus domestica and Arabidopsis thaliana to explore the function of PpSAT4 in adventitious root formation. [Results] (1) Peach PpSAT4 gene with a sequence of 864 bp was cloned, encoding a protein with a molecular weight of 30.49 kD and an isoelectric point of 6.70. Phylogenetic analysis showed that PpSAT4 had the closest relationship with European sweet cherries and almonds. Analysis of cis-acting elements showed the presence of response elements such as light, auxin, and gibberellin in the PpSAT4 sequence. (2) The overexpression vector Pp- SAT4-CAM-FLAG was constructed and transformed P . domestica and A . thaliana, and the transgenic lines were obtained. (3) qRT-PCR analysis showed that the expression level of PpSAT4 in the transgenic lines was higher than that of the wild-type. Overexpression of PpSAT4 increased various indices and POD and SOD activities in the plum root system. In addition, overexpression of this gene in A . thaliana increased root length and enzyme activity, suggesting a similar function in A . thaliana. [Conclusion] Pp- SAT4 plays a crucial role in facilitating the formation of adventitious roots.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240400?st=article_issuepeach rootstock ‘gf 677’gene cloningadventitious rootgenetic transformationfunctional verification |
| spellingShingle | HAO Lanlan WANG Hong ZHANG Fan ZHANG Xuebing YANG Yang Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ Xibei zhiwu xuebao peach rootstock ‘gf 677’ gene cloning adventitious root genetic transformation functional verification |
| title | Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ |
| title_full | Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ |
| title_fullStr | Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ |
| title_full_unstemmed | Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ |
| title_short | Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ |
| title_sort | functional study on the regulation of adventitious root formation by ppsat4 gene in peach rootstock gf 677 |
| topic | peach rootstock ‘gf 677’ gene cloning adventitious root genetic transformation functional verification |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240400?st=article_issue |
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