Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’
[Objective] The study aims to explore the function of SAT4 (serine acetyltransferase 4), a member of the peach serine acetyltransferase family, in adventitious root formation, and provide potential target genes for future breeding to improve rooting in peach. [Methods] Using the transcriptome of a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Science Press
2025-01-01
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| Series: | Xibei zhiwu xuebao |
| Subjects: | |
| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240400?st=article_issue |
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| Summary: | [Objective] The study aims to explore the function of SAT4 (serine acetyltransferase 4), a
member of the peach serine acetyltransferase family, in adventitious root formation, and provide potential
target genes for future breeding to improve rooting in peach. [Methods] Using the transcriptome of adventitious
roots, the PpSAT4 gene was screened and transformed into Prunus domestica and Arabidopsis
thaliana to explore the function of PpSAT4 in adventitious root formation. [Results] (1) Peach PpSAT4
gene with a sequence of 864 bp was cloned, encoding a protein with a molecular weight of 30.49 kD and an
isoelectric point of 6.70. Phylogenetic analysis showed that PpSAT4 had the closest relationship with European
sweet cherries and almonds. Analysis of cis-acting elements showed the presence of response elements
such as light, auxin, and gibberellin in the PpSAT4 sequence. (2) The overexpression vector Pp-
SAT4-CAM-FLAG was constructed and transformed P . domestica and A . thaliana, and the transgenic
lines were obtained. (3) qRT-PCR analysis showed that the expression level of PpSAT4 in the transgenic
lines was higher than that of the wild-type. Overexpression of PpSAT4 increased various indices and POD
and SOD activities in the plum root system. In addition, overexpression of this gene in A . thaliana increased
root length and enzyme activity, suggesting a similar function in A . thaliana. [Conclusion] Pp-
SAT4 plays a crucial role in facilitating the formation of adventitious roots. |
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| ISSN: | 1000-4025 |