Growth of white shrimp post-larvae immersed in recombinant fish growth hormone

ABSTRACT   This research was conducted to determine the optimum immersion time of recombinant giant grouper growth hormone (Ephinephelus lanceolatus; rElGH) at a dose of 15 mg/L that generated highest growth of white shrimp post-larvae (PL). PL was bath-immersed for one, two, and three hours. Two ty...

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Main Authors: Dita Puji Laksana, Siti Subaidah, Muhammad Zairin Junior, , Alimuddin, Odang Carman
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2015-05-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/9359
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author Dita Puji Laksana
Siti Subaidah
Muhammad Zairin Junior
, Alimuddin
Odang Carman
author_facet Dita Puji Laksana
Siti Subaidah
Muhammad Zairin Junior
, Alimuddin
Odang Carman
author_sort Dita Puji Laksana
collection DOAJ
description ABSTRACT   This research was conducted to determine the optimum immersion time of recombinant giant grouper growth hormone (Ephinephelus lanceolatus; rElGH) at a dose of 15 mg/L that generated highest growth of white shrimp post-larvae (PL). PL was bath-immersed for one, two, and three hours. Two types of control was provided, namely it was without any treatment (control), and immersion in water containing 0.01% bovine serum albumin (BSA) and total protein of Escherichia coli without rElGH (pCold control). All treatments and controls were consisted of three replications. A total of 1,500 PL-2 shrimp were bath-immersed in a plastic packing containing 1 L of sea water, 15 mg/L rElGH, and 0.01% BSA. PL was further reared for 21 days in the 60 L glass aquarium, and fed nauplii Artemia two times and flake commercial diet five times daily, at satiation. The results showed that the highest of biomass (36.29±1.46 g), specific growth rate (29.81±0.87%/day), and body length (20.08±0.42 mm) were obtained in three hours immersion treatment (P<0.05). Biomass of PL in three hours immersion treatment was approximately 66.0% higher compared to the control (21.87±2.53 g). Survival of shrimp in all treatment and control were similar (P>0.05). Thus, growth of white shrimp PL could be improved by bath immersion for three hours in rElGH solution of 15 mg/L water.   Keywords: recombinant growth hormone, different immersion time, Pacific white shrimp, biomass     ABSTRAK   Penelitian ini dilakukan untuk menentukan lama waktu perendaman hormon pertumbuhan rekombinan ikan kerapu kertang (Ephinephelus lanceolatus; rElGH) dosis 15 mg/L yang menghasilkan pertumbuhan tertinggi pada pascalarva (PL) udang vaname. Perendaman PL dilakukan satu, dua, dan tiga jam. Dua jenis kontrol dibuat yakni udang vaname PL-2 tidak diberi perlakuan (kontrol), dan direndam dalam air mengandung serum albumin sapi (BSA) 0,01% dan protein Escherichia coli tanpa rElGH (kontrol pCold). Setiap perlakuan dan kontrol diberi tiga ulangan. Sebanyak 1.500 ekor PL-2 direndam dalam kantong plastik kemasan berisi 1 L air laut mengandung rElGH 15 mg/L, dan BSA 0,01%. Selanjutnya, udang dipelihara selama 21 hari di dalam akuarium volume 60 L, dan diberi pakan naupli Artemia sebanyak dua kali dan pakan komersial berbentuk flake sebanyak lima kali sehari hingga kenyang. Hasil penelitian menunjukkan bahwa biomassa (36,29±1,46 g), pertumbuhan bobot spesifik (29,81±0,87%/hari), dan panjang tubuh (20,08±0,42 mm) tertinggi diperoleh pada perlakuan perendaman selama tiga jam (P<0,05). Biomassa udang perlakuan perendaman selama tiga jam lebih tinggi 66% dibandingkan dengan kontrol (21,87±2,53 g). Kelangsungan hidup udang yang direndam dengan rElGH, kontrol dan kontrol pCold tidak berbeda nyata (P<0,05). Dengan demikian, pertumbuhan PL udang vaname dapat ditingkatkan melalui perendaman selama tiga jam dalam larutan rElGH 15 mg/L air.   Kata kunci: hormon pertumbuhan rekombinan, lama perendaman, pascalarva udang vaname, biomassa
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spelling doaj-art-956ad9101bfb402bb0ddd27710f62d532025-01-24T17:59:27ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-011227826Growth of white shrimp post-larvae immersed in recombinant fish growth hormoneDita Puji Laksana0Siti Subaidah1Muhammad Zairin Junior2, Alimuddin3Odang Carman4Department of Aquaculture, Bogor Agricultural UniversityDepartment of Aquaculture, Bogor Agricultural UniversityDepartment of Aquaculture, Bogor Agricultural UniversityDepartment of Aquaculture, Bogor Agricultural UniversityDepartment of Aquaculture, Bogor Agricultural UniversityABSTRACT   This research was conducted to determine the optimum immersion time of recombinant giant grouper growth hormone (Ephinephelus lanceolatus; rElGH) at a dose of 15 mg/L that generated highest growth of white shrimp post-larvae (PL). PL was bath-immersed for one, two, and three hours. Two types of control was provided, namely it was without any treatment (control), and immersion in water containing 0.01% bovine serum albumin (BSA) and total protein of Escherichia coli without rElGH (pCold control). All treatments and controls were consisted of three replications. A total of 1,500 PL-2 shrimp were bath-immersed in a plastic packing containing 1 L of sea water, 15 mg/L rElGH, and 0.01% BSA. PL was further reared for 21 days in the 60 L glass aquarium, and fed nauplii Artemia two times and flake commercial diet five times daily, at satiation. The results showed that the highest of biomass (36.29±1.46 g), specific growth rate (29.81±0.87%/day), and body length (20.08±0.42 mm) were obtained in three hours immersion treatment (P<0.05). Biomass of PL in three hours immersion treatment was approximately 66.0% higher compared to the control (21.87±2.53 g). Survival of shrimp in all treatment and control were similar (P>0.05). Thus, growth of white shrimp PL could be improved by bath immersion for three hours in rElGH solution of 15 mg/L water.   Keywords: recombinant growth hormone, different immersion time, Pacific white shrimp, biomass     ABSTRAK   Penelitian ini dilakukan untuk menentukan lama waktu perendaman hormon pertumbuhan rekombinan ikan kerapu kertang (Ephinephelus lanceolatus; rElGH) dosis 15 mg/L yang menghasilkan pertumbuhan tertinggi pada pascalarva (PL) udang vaname. Perendaman PL dilakukan satu, dua, dan tiga jam. Dua jenis kontrol dibuat yakni udang vaname PL-2 tidak diberi perlakuan (kontrol), dan direndam dalam air mengandung serum albumin sapi (BSA) 0,01% dan protein Escherichia coli tanpa rElGH (kontrol pCold). Setiap perlakuan dan kontrol diberi tiga ulangan. Sebanyak 1.500 ekor PL-2 direndam dalam kantong plastik kemasan berisi 1 L air laut mengandung rElGH 15 mg/L, dan BSA 0,01%. Selanjutnya, udang dipelihara selama 21 hari di dalam akuarium volume 60 L, dan diberi pakan naupli Artemia sebanyak dua kali dan pakan komersial berbentuk flake sebanyak lima kali sehari hingga kenyang. Hasil penelitian menunjukkan bahwa biomassa (36,29±1,46 g), pertumbuhan bobot spesifik (29,81±0,87%/hari), dan panjang tubuh (20,08±0,42 mm) tertinggi diperoleh pada perlakuan perendaman selama tiga jam (P<0,05). Biomassa udang perlakuan perendaman selama tiga jam lebih tinggi 66% dibandingkan dengan kontrol (21,87±2,53 g). Kelangsungan hidup udang yang direndam dengan rElGH, kontrol dan kontrol pCold tidak berbeda nyata (P<0,05). Dengan demikian, pertumbuhan PL udang vaname dapat ditingkatkan melalui perendaman selama tiga jam dalam larutan rElGH 15 mg/L air.   Kata kunci: hormon pertumbuhan rekombinan, lama perendaman, pascalarva udang vaname, biomassa https://journal.ipb.ac.id/index.php/jai/article/view/9359
spellingShingle Dita Puji Laksana
Siti Subaidah
Muhammad Zairin Junior
, Alimuddin
Odang Carman
Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
Jurnal Akuakultur Indonesia
title Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
title_full Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
title_fullStr Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
title_full_unstemmed Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
title_short Growth of white shrimp post-larvae immersed in recombinant fish growth hormone
title_sort growth of white shrimp post larvae immersed in recombinant fish growth hormone
url https://journal.ipb.ac.id/index.php/jai/article/view/9359
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AT muhammadzairinjunior growthofwhiteshrimppostlarvaeimmersedinrecombinantfishgrowthhormone
AT alimuddin growthofwhiteshrimppostlarvaeimmersedinrecombinantfishgrowthhormone
AT odangcarman growthofwhiteshrimppostlarvaeimmersedinrecombinantfishgrowthhormone