Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis
Introduction. Malignant brain tumors, such as anaplastic astrocytomas and anaplastic oligodendrogliomas grade 3, are characterized by high aggressiveness and pose a serious clinical problem. This study focuses on assessing intratumoral heterogeneity in anaplastic astrocytomas and anaplastic oligoden...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
ABV-press
2023-12-01
|
| Series: | Опухоли головы и шеи |
| Subjects: | |
| Online Access: | https://ogsh.abvpress.ru/jour/article/view/914 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849401784665112576 |
|---|---|
| author | P. V. Nikitin A. Yu. Belyaev G. R. Musina G. L. Kobyakov I. N. Pronin D. Yu. Usachev |
| author_facet | P. V. Nikitin A. Yu. Belyaev G. R. Musina G. L. Kobyakov I. N. Pronin D. Yu. Usachev |
| author_sort | P. V. Nikitin |
| collection | DOAJ |
| description | Introduction. Malignant brain tumors, such as anaplastic astrocytomas and anaplastic oligodendrogliomas grade 3, are characterized by high aggressiveness and pose a serious clinical problem. This study focuses on assessing intratumoral heterogeneity in anaplastic astrocytomas and anaplastic oligodendrogliomas and its impact on disease prognosis.Aim. To study characteristics of intratumoral heterogeneity, in particular such morphological criteria as necrosis, vascular proliferation, mitoses, and mutations in the most significant for glioma progression genes in the groups of grade III astrocytomas and oligodendrogliomas, as well as analysis of prognostic significance of these parameters.Materials and methods. The study included 389 patients with IDH-mutant astrocytomas and 200 patients with oligodendrogliomas. The mean Ki-67 labeling index of astrocytomas was 12.78 %, while that of oligodendrogliomas was 8.54 %.Results. The presence of vascular proliferation, necrosis, of more than 20 % of the area of the specimen occupied by sarcomatous-like areas and the number of mitoses significantly affected not only disease-free survival but also overall survival of patients. In the clinical setting, mutations in the TERT promoter gene, amplification and mutation of the EGFR gene, deletion of the CDKN2A gene, and TP53 gene had a significant negative impact on recurrence-free and overall survival.Conclusion. The results of single-cell RNA sequencing showed additional factors, including sarcomatous-like areas, as well as TERT, EGFR, CDKN2A and TP53 mutations, in the progression of the tumors under consideration and in ensuring an increase in their malignant potential. |
| format | Article |
| id | doaj-art-955ee33419b64ae58dda12da17396cbe |
| institution | Kabale University |
| issn | 2222-1468 2411-4634 |
| language | Russian |
| publishDate | 2023-12-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли головы и шеи |
| spelling | doaj-art-955ee33419b64ae58dda12da17396cbe2025-08-20T03:37:42ZrusABV-pressОпухоли головы и шеи2222-14682411-46342023-12-01133516210.17650/2222-1468-2023-13-3-51-62561Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosisP. V. Nikitin0A. Yu. Belyaev1G. R. Musina2G. L. Kobyakov3I. N. Pronin4D. Yu. Usachev5University of HoustonN.N. Burdenko National Medical Research Center of Neurosurgery, of the Ministry of Health of RussiaBaylor Medical CollegeN.N. Burdenko National Medical Research Center of Neurosurgery, of the Ministry of Health of RussiaN.N. Burdenko National Medical Research Center of Neurosurgery, of the Ministry of Health of RussiaN.N. Burdenko National Medical Research Center of Neurosurgery, of the Ministry of Health of RussiaIntroduction. Malignant brain tumors, such as anaplastic astrocytomas and anaplastic oligodendrogliomas grade 3, are characterized by high aggressiveness and pose a serious clinical problem. This study focuses on assessing intratumoral heterogeneity in anaplastic astrocytomas and anaplastic oligodendrogliomas and its impact on disease prognosis.Aim. To study characteristics of intratumoral heterogeneity, in particular such morphological criteria as necrosis, vascular proliferation, mitoses, and mutations in the most significant for glioma progression genes in the groups of grade III astrocytomas and oligodendrogliomas, as well as analysis of prognostic significance of these parameters.Materials and methods. The study included 389 patients with IDH-mutant astrocytomas and 200 patients with oligodendrogliomas. The mean Ki-67 labeling index of astrocytomas was 12.78 %, while that of oligodendrogliomas was 8.54 %.Results. The presence of vascular proliferation, necrosis, of more than 20 % of the area of the specimen occupied by sarcomatous-like areas and the number of mitoses significantly affected not only disease-free survival but also overall survival of patients. In the clinical setting, mutations in the TERT promoter gene, amplification and mutation of the EGFR gene, deletion of the CDKN2A gene, and TP53 gene had a significant negative impact on recurrence-free and overall survival.Conclusion. The results of single-cell RNA sequencing showed additional factors, including sarcomatous-like areas, as well as TERT, EGFR, CDKN2A and TP53 mutations, in the progression of the tumors under consideration and in ensuring an increase in their malignant potential.https://ogsh.abvpress.ru/jour/article/view/914astrocytoma grade 3oligodendroglioma grade 3intra-tumoral heterogeneityoverall survivalprogressionfree survival |
| spellingShingle | P. V. Nikitin A. Yu. Belyaev G. R. Musina G. L. Kobyakov I. N. Pronin D. Yu. Usachev Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis Опухоли головы и шеи astrocytoma grade 3 oligodendroglioma grade 3 intra-tumoral heterogeneity overall survival progressionfree survival |
| title | Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| title_full | Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| title_fullStr | Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| title_full_unstemmed | Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| title_short | Intra-tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| title_sort | intra tumoral molecular heterogeneity of grade 3 astrocytomas and oligodendrogliomas and its significance in disease prognosis |
| topic | astrocytoma grade 3 oligodendroglioma grade 3 intra-tumoral heterogeneity overall survival progressionfree survival |
| url | https://ogsh.abvpress.ru/jour/article/view/914 |
| work_keys_str_mv | AT pvnikitin intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis AT ayubelyaev intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis AT grmusina intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis AT glkobyakov intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis AT inpronin intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis AT dyuusachev intratumoralmolecularheterogeneityofgrade3astrocytomasandoligodendrogliomasanditssignificanceindiseaseprognosis |