Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study
Background: This study was undertaken to evaluate the effect of cryopreservation on the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles using sperm retrieved from testicular sperm extraction (TESE) in patients with azoospermia. Methods: This retrospective analysis included the cl...
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IMR Press
2022-10-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911239 |
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| author | Yali Wang Dongjia Chen Bing Cai Dan Huang Yanwen Xu Chenhui Ding |
| author_facet | Yali Wang Dongjia Chen Bing Cai Dan Huang Yanwen Xu Chenhui Ding |
| author_sort | Yali Wang |
| collection | DOAJ |
| description | Background: This study was undertaken to evaluate the effect of cryopreservation on the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles using sperm retrieved from testicular sperm extraction (TESE) in patients with azoospermia. Methods: This retrospective analysis included the clinical data of 56 pairs of fresh and frozen sperm injection cycles from 56 couples after TESE from January 2019 to December 2021 at the Reproductive Medicine Center of the First Affiliated Hospital of Sun Yat-sen University, of which 42 pairs were ICSI cycles using fresh and frozen sperm from the same TESE procedure. We compared the embryological and laboratory characteristics and pregnancy outcomes of the subsequent first embryo transfer (ET) cycles between the fresh and frozen groups. Results: There were no significant differences in the fertilization, cleavage, good-quality day 3 embryo, blastocyst formation, and good-quality blastocyst rates between the groups. However, when only paired ICSI cycles of fresh and frozen sperm from the same TESE procedure were analyzed, we observed that the good-quality day 3 embryo rate (44.8% vs 33.2%, p = 0.029) and blastocyst formation rate (57.5% vs 41.3%, p = 0.028) in the fresh group were significantly higher than those in the frozen group. Implantation, clinical pregnancy, early miscarriage, and live birth rates of the first ET cycle were not significantly different in either group. Conclusions: ICSI using fresh testicular sperm after TESE in patients with azoospermia appears to yield better embryological and laboratory outcomes than ICSI using cryopreserved testicular sperm, but the success rate of the subsequent first ET cycles does not seem to be affected. |
| format | Article |
| id | doaj-art-9556b1088a2146fbb768ae3af9b26da4 |
| institution | Kabale University |
| issn | 0390-6663 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-9556b1088a2146fbb768ae3af9b26da42025-08-20T03:48:31ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632022-10-01491123910.31083/j.ceog4911239S0390-6663(22)01890-5Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional StudyYali Wang0Dongjia Chen1Bing Cai2Dan Huang3Yanwen Xu4Chenhui Ding5Reproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaReproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaReproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaReproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaReproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaReproductive Medicine Center, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, Guangdong, ChinaBackground: This study was undertaken to evaluate the effect of cryopreservation on the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles using sperm retrieved from testicular sperm extraction (TESE) in patients with azoospermia. Methods: This retrospective analysis included the clinical data of 56 pairs of fresh and frozen sperm injection cycles from 56 couples after TESE from January 2019 to December 2021 at the Reproductive Medicine Center of the First Affiliated Hospital of Sun Yat-sen University, of which 42 pairs were ICSI cycles using fresh and frozen sperm from the same TESE procedure. We compared the embryological and laboratory characteristics and pregnancy outcomes of the subsequent first embryo transfer (ET) cycles between the fresh and frozen groups. Results: There were no significant differences in the fertilization, cleavage, good-quality day 3 embryo, blastocyst formation, and good-quality blastocyst rates between the groups. However, when only paired ICSI cycles of fresh and frozen sperm from the same TESE procedure were analyzed, we observed that the good-quality day 3 embryo rate (44.8% vs 33.2%, p = 0.029) and blastocyst formation rate (57.5% vs 41.3%, p = 0.028) in the fresh group were significantly higher than those in the frozen group. Implantation, clinical pregnancy, early miscarriage, and live birth rates of the first ET cycle were not significantly different in either group. Conclusions: ICSI using fresh testicular sperm after TESE in patients with azoospermia appears to yield better embryological and laboratory outcomes than ICSI using cryopreserved testicular sperm, but the success rate of the subsequent first ET cycles does not seem to be affected.https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911239azoospermiaspermcryopreservationtesticular sperm extractionintracytoplasmic sperm injectionpregnancy |
| spellingShingle | Yali Wang Dongjia Chen Bing Cai Dan Huang Yanwen Xu Chenhui Ding Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study Clinical and Experimental Obstetrics & Gynecology azoospermia sperm cryopreservation testicular sperm extraction intracytoplasmic sperm injection pregnancy |
| title | Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study |
| title_full | Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study |
| title_fullStr | Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study |
| title_full_unstemmed | Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study |
| title_short | Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study |
| title_sort | impact of spermatozoa cryopreservation on clinical outcomes of intracytoplasmic sperm injection in patients with azoospermia a retrospective cross sectional study |
| topic | azoospermia sperm cryopreservation testicular sperm extraction intracytoplasmic sperm injection pregnancy |
| url | https://www.imrpress.com/journal/CEOG/49/11/10.31083/j.ceog4911239 |
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