Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California.
There is a need for a regional assessment of the frequency and diversity of MRSA to determine major circulating clones and the extent to which community and healthcare MRSA reservoirs have mixed. We conducted a prospective cohort study of inpatients in Orange County, California, systematically colle...
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Public Library of Science (PLoS)
2013-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0062117 |
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| author | Lyndsey O Hudson Courtney R Murphy Brian G Spratt Mark C Enright Kristen Elkins Christopher Nguyen Leah Terpstra Adrijana Gombosev Diane Kim Paul Hannah Lydia Mikhail Richard Alexander Douglas F Moore Susan S Huang |
| author_facet | Lyndsey O Hudson Courtney R Murphy Brian G Spratt Mark C Enright Kristen Elkins Christopher Nguyen Leah Terpstra Adrijana Gombosev Diane Kim Paul Hannah Lydia Mikhail Richard Alexander Douglas F Moore Susan S Huang |
| author_sort | Lyndsey O Hudson |
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| description | There is a need for a regional assessment of the frequency and diversity of MRSA to determine major circulating clones and the extent to which community and healthcare MRSA reservoirs have mixed. We conducted a prospective cohort study of inpatients in Orange County, California, systematically collecting clinical MRSA isolates from 30 hospitals, to assess MRSA diversity and distribution. All isolates were characterized by spa typing, with selective PFGE and MLST to relate spa types with major MRSA clones. We collected 2,246 MRSA isolates from hospital inpatients. This translated to 91/10,000 inpatients with MRSA and an Orange County population estimate of MRSA inpatient clinical cultures of 86/100,000 people. spa type genetic diversity was heterogeneous between hospitals, and relatively high overall (72%). USA300 (t008/ST8), USA100 (t002/ST5) and a previously reported USA100 variant (t242/ST5) were the dominant clones across all Orange County hospitals, representing 83% of isolates. Fifteen hospitals isolated more t008 (USA300) isolates than t002/242 (USA100) isolates, and 12 hospitals isolated more t242 isolates than t002 isolates. The majority of isolates were imported into hospitals. Community-based infection control strategies may still be helpful in stemming the influx of traditionally community-associated strains, particularly USA300, into the healthcare setting. |
| format | Article |
| id | doaj-art-955314fd78b7441ab085ee77ce683f45 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-955314fd78b7441ab085ee77ce683f452025-08-20T02:30:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6211710.1371/journal.pone.0062117Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California.Lyndsey O HudsonCourtney R MurphyBrian G SprattMark C EnrightKristen ElkinsChristopher NguyenLeah TerpstraAdrijana GombosevDiane KimPaul HannahLydia MikhailRichard AlexanderDouglas F MooreSusan S HuangThere is a need for a regional assessment of the frequency and diversity of MRSA to determine major circulating clones and the extent to which community and healthcare MRSA reservoirs have mixed. We conducted a prospective cohort study of inpatients in Orange County, California, systematically collecting clinical MRSA isolates from 30 hospitals, to assess MRSA diversity and distribution. All isolates were characterized by spa typing, with selective PFGE and MLST to relate spa types with major MRSA clones. We collected 2,246 MRSA isolates from hospital inpatients. This translated to 91/10,000 inpatients with MRSA and an Orange County population estimate of MRSA inpatient clinical cultures of 86/100,000 people. spa type genetic diversity was heterogeneous between hospitals, and relatively high overall (72%). USA300 (t008/ST8), USA100 (t002/ST5) and a previously reported USA100 variant (t242/ST5) were the dominant clones across all Orange County hospitals, representing 83% of isolates. Fifteen hospitals isolated more t008 (USA300) isolates than t002/242 (USA100) isolates, and 12 hospitals isolated more t242 isolates than t002 isolates. The majority of isolates were imported into hospitals. Community-based infection control strategies may still be helpful in stemming the influx of traditionally community-associated strains, particularly USA300, into the healthcare setting.https://doi.org/10.1371/journal.pone.0062117 |
| spellingShingle | Lyndsey O Hudson Courtney R Murphy Brian G Spratt Mark C Enright Kristen Elkins Christopher Nguyen Leah Terpstra Adrijana Gombosev Diane Kim Paul Hannah Lydia Mikhail Richard Alexander Douglas F Moore Susan S Huang Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. PLoS ONE |
| title | Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. |
| title_full | Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. |
| title_fullStr | Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. |
| title_full_unstemmed | Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. |
| title_short | Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California. |
| title_sort | diversity of methicillin resistant staphylococcus aureus mrsa strains isolated from inpatients of 30 hospitals in orange county california |
| url | https://doi.org/10.1371/journal.pone.0062117 |
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