Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots.

Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots.

<h4>Background</h4>Zoonotic P. knowlesi and P. cynomolgi symptomatic and asymptomatic infections occur across endemic areas of Southeast Asia. Most infections are low-parasitemia, with an unknown proportion below routine microscopy detection thresholds. Molecular surveillance tools optim...

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Main Authors: Kamil A Braima, Kim A Piera, Inke N D Lubis, Rintis Noviyanti, Giri S Rajahram, Pinkan Kariodimedjo, Irbah R A Nainggolan, Ranti Permatasari, Leily Trianty, Ristya Amalia, Sitti Saimah Sakam, Angelica F Tan, Timothy William, Jacob A F Westaway, PingChin Lee, Sylvia Daim, Henry Surendra, Nathaniel Christy, Andrew G Letizia, Christopher L Peatey, Mohd Arshil Moideen, Bridget E Barber, Colin J Sutherland, Nicholas M Anstey, Matthew J Grigg
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-02-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0012129
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