Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro
Dillenia indica is a medicinal plant used in the indigenous system of medicine for plethora of diseases. In this study, the efficacy of ethanolic bark extract of Dillenia indica was investigated for green synthesis of copper nanoparticles (CuNPs). Obtained CuNPs were crystalline, spherical in shape...
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Experimental Nanoscience |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17458080.2023.2249241 |
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| author | Larica Mohanta Bhabani Sankar Jena |
| author_facet | Larica Mohanta Bhabani Sankar Jena |
| author_sort | Larica Mohanta |
| collection | DOAJ |
| description | Dillenia indica is a medicinal plant used in the indigenous system of medicine for plethora of diseases. In this study, the efficacy of ethanolic bark extract of Dillenia indica was investigated for green synthesis of copper nanoparticles (CuNPs). Obtained CuNPs were crystalline, spherical in shape within a size range of 20.87–45.73 nm, well dispersed showing strong negative zeta potential value (−41.8 mV) as analysed by XRD, TEM, FESEM and particle size analyser. The elemental composition of CuNPs was examined through EDX spectrum followed by field map analysis. FTIR spectra showed the presence of active biomolecules responsible for reduction of copper ions as well as for surface functionalisation of CuNPs. Synthesised CuNPs showed marked free radical scavenging efficacy at IC50 of 37.2 µg/mL. MTT assay in CuNPs treated A549 and MCF-7 cancer cell lines for 24 and 48 h showed substantial decrease of cell viability with increase in concentration. Further, morphological alterations like cell shrinkage, nuclear fragmentation and blebbing were remarkably observed in CuNPs treated both the cell lines as assayed through AO/EtBr and DAPI staining methods. Thus, D. indica bark extract-mediated CuNPs were potential free radical scavengers and effective cytotoxic against A549 and MCF-7 cancer cell lines. |
| format | Article |
| id | doaj-art-954888d8fbcb4c8592ad855d24ce11a1 |
| institution | OA Journals |
| issn | 1745-8080 1745-8099 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Journal of Experimental Nanoscience |
| spelling | doaj-art-954888d8fbcb4c8592ad855d24ce11a12025-08-20T02:23:41ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992023-12-0118110.1080/17458080.2023.2249241Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitroLarica Mohanta0Bhabani Sankar Jena1CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, IndiaCSIR-Institute of Minerals and Materials Technology, Bhubaneswar, IndiaDillenia indica is a medicinal plant used in the indigenous system of medicine for plethora of diseases. In this study, the efficacy of ethanolic bark extract of Dillenia indica was investigated for green synthesis of copper nanoparticles (CuNPs). Obtained CuNPs were crystalline, spherical in shape within a size range of 20.87–45.73 nm, well dispersed showing strong negative zeta potential value (−41.8 mV) as analysed by XRD, TEM, FESEM and particle size analyser. The elemental composition of CuNPs was examined through EDX spectrum followed by field map analysis. FTIR spectra showed the presence of active biomolecules responsible for reduction of copper ions as well as for surface functionalisation of CuNPs. Synthesised CuNPs showed marked free radical scavenging efficacy at IC50 of 37.2 µg/mL. MTT assay in CuNPs treated A549 and MCF-7 cancer cell lines for 24 and 48 h showed substantial decrease of cell viability with increase in concentration. Further, morphological alterations like cell shrinkage, nuclear fragmentation and blebbing were remarkably observed in CuNPs treated both the cell lines as assayed through AO/EtBr and DAPI staining methods. Thus, D. indica bark extract-mediated CuNPs were potential free radical scavengers and effective cytotoxic against A549 and MCF-7 cancer cell lines.https://www.tandfonline.com/doi/10.1080/17458080.2023.2249241Green synthesisDillenia indicacopper nanoparticlesantioxidantanticancer |
| spellingShingle | Larica Mohanta Bhabani Sankar Jena Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro Journal of Experimental Nanoscience Green synthesis Dillenia indica copper nanoparticles antioxidant anticancer |
| title | Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro |
| title_full | Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro |
| title_fullStr | Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro |
| title_full_unstemmed | Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro |
| title_short | Dillenia indica bark extract mediated bio-fabrication of copper nanoparticles: characterisation, antioxidant and anticancer activity in vitro |
| title_sort | dillenia indica bark extract mediated bio fabrication of copper nanoparticles characterisation antioxidant and anticancer activity in vitro |
| topic | Green synthesis Dillenia indica copper nanoparticles antioxidant anticancer |
| url | https://www.tandfonline.com/doi/10.1080/17458080.2023.2249241 |
| work_keys_str_mv | AT laricamohanta dilleniaindicabarkextractmediatedbiofabricationofcoppernanoparticlescharacterisationantioxidantandanticanceractivityinvitro AT bhabanisankarjena dilleniaindicabarkextractmediatedbiofabricationofcoppernanoparticlescharacterisationantioxidantandanticanceractivityinvitro |