MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold
The effect of dynamic compressive stimulation on MG-63 cell proliferation on an auxetic PLGA scaffold was investigated. The estimated Poisson ratio of the prepared auxetic scaffold specimens was approximately (−)0.07, while the Poisson ratio estimated for conventional scaffold specimens was (+)0.12...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2017/1286109 |
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| _version_ | 1832558543397453824 |
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| author | Myeong Jin Kim Hong Jin Choi Jongman Cho Jung Bok Lee Hak-Joon Sung Jeong Koo Kim |
| author_facet | Myeong Jin Kim Hong Jin Choi Jongman Cho Jung Bok Lee Hak-Joon Sung Jeong Koo Kim |
| author_sort | Myeong Jin Kim |
| collection | DOAJ |
| description | The effect of dynamic compressive stimulation on MG-63 cell proliferation on an auxetic PLGA scaffold was investigated. The estimated Poisson ratio of the prepared auxetic scaffold specimens was approximately (−)0.07, while the Poisson ratio estimated for conventional scaffold specimens was (+)0.12 under 10% strain compression on average. Three stimulus groups were examined: control (no stimulation), static compression, and dynamic compression. In preparation for proliferation testing, cells were seeded at 2.2 × 105 cells/80 μL on each scaffold specimen. The average proliferation rates of the static and dynamic groups were higher than those of the control group: 13.4% and 25.5% higher at culture day 1, 34.7% and 56.2% at culture day 3, and 17.5% and 43.0% at culture day 5, respectively. The static and dynamic group results at culture day 5 were significantly different (p<0.01). Moreover, proliferation rate of the dynamic stimulation group was 1.22 times higher than that of the static group (p<0.01). Conclusively, proliferation of osteoblast-like cells was enhanced through compressive stimulation, but the enhancement was maximal with dynamic compressive stimulation of auxetic scaffolds. |
| format | Article |
| id | doaj-art-953874ab00794422a5cd797c681db104 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-953874ab00794422a5cd797c681db1042025-02-03T01:32:14ZengWileyInternational Journal of Polymer Science1687-94221687-94302017-01-01201710.1155/2017/12861091286109MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA ScaffoldMyeong Jin Kim0Hong Jin Choi1Jongman Cho2Jung Bok Lee3Hak-Joon Sung4Jeong Koo Kim5School of Biomedical Engineering, Inje University, Obang-Dong, Gimhae, Gyeongnam 621-749, Republic of KoreaSchool of Biomedical Engineering, Inje University, Obang-Dong, Gimhae, Gyeongnam 621-749, Republic of KoreaSchool of Biomedical Engineering, Inje University, Obang-Dong, Gimhae, Gyeongnam 621-749, Republic of KoreaDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USADepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USASchool of Biomedical Engineering, Inje University, Obang-Dong, Gimhae, Gyeongnam 621-749, Republic of KoreaThe effect of dynamic compressive stimulation on MG-63 cell proliferation on an auxetic PLGA scaffold was investigated. The estimated Poisson ratio of the prepared auxetic scaffold specimens was approximately (−)0.07, while the Poisson ratio estimated for conventional scaffold specimens was (+)0.12 under 10% strain compression on average. Three stimulus groups were examined: control (no stimulation), static compression, and dynamic compression. In preparation for proliferation testing, cells were seeded at 2.2 × 105 cells/80 μL on each scaffold specimen. The average proliferation rates of the static and dynamic groups were higher than those of the control group: 13.4% and 25.5% higher at culture day 1, 34.7% and 56.2% at culture day 3, and 17.5% and 43.0% at culture day 5, respectively. The static and dynamic group results at culture day 5 were significantly different (p<0.01). Moreover, proliferation rate of the dynamic stimulation group was 1.22 times higher than that of the static group (p<0.01). Conclusively, proliferation of osteoblast-like cells was enhanced through compressive stimulation, but the enhancement was maximal with dynamic compressive stimulation of auxetic scaffolds.http://dx.doi.org/10.1155/2017/1286109 |
| spellingShingle | Myeong Jin Kim Hong Jin Choi Jongman Cho Jung Bok Lee Hak-Joon Sung Jeong Koo Kim MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold International Journal of Polymer Science |
| title | MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold |
| title_full | MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold |
| title_fullStr | MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold |
| title_full_unstemmed | MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold |
| title_short | MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold |
| title_sort | mg 63 cell proliferation with static or dynamic compressive stimulation on an auxetic plga scaffold |
| url | http://dx.doi.org/10.1155/2017/1286109 |
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