Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria
Nanoparticles with bactericidal properties offer significant advantages, and their integration with other nanomaterials can further enhance these benefits. This study investigates the synergistic effects of Ag-doped ZnO/AgO (ZnAg) nanocomposites combined with TiO2 nanocrystals (NCs) as antibacterial...
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Elsevier
2025-04-01
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| Series: | Next Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949822824003459 |
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| author | Rafael O. Trevisan Jerusa M. Oliveira Hugo F. Perini Ulisses Travaglini Thaís K. de L. Rezende Francisco R.A. dos Santos Luciana R. de S. Floresta Ana L.S. Borges Leticia C. Ruiz Leonardo E. de A. e Silva Juliane Z. Marinho Fernanda M. Fonseca Carlo J.F. de Oliveira Virmondes R. Júnior Marcos V. da Silva Lucas Anhezini Anielle Christine A. Silva |
| author_facet | Rafael O. Trevisan Jerusa M. Oliveira Hugo F. Perini Ulisses Travaglini Thaís K. de L. Rezende Francisco R.A. dos Santos Luciana R. de S. Floresta Ana L.S. Borges Leticia C. Ruiz Leonardo E. de A. e Silva Juliane Z. Marinho Fernanda M. Fonseca Carlo J.F. de Oliveira Virmondes R. Júnior Marcos V. da Silva Lucas Anhezini Anielle Christine A. Silva |
| author_sort | Rafael O. Trevisan |
| collection | DOAJ |
| description | Nanoparticles with bactericidal properties offer significant advantages, and their integration with other nanomaterials can further enhance these benefits. This study investigates the synergistic effects of Ag-doped ZnO/AgO (ZnAg) nanocomposites combined with TiO2 nanocrystals (NCs) as antibacterial agents while assessing their in vitro and in vivo nanotoxicity. Three distinct compositions (25Ti/75ZnAg, 50Ti/50ZnAg, and 75Ti/25ZnAg) were evaluated for their combined effects. Characterization through X-ray diffraction (XRD) and scanning electron microscopy (SEM) confirmed the successful synthesis of nanocomposites with well-dispersed NCs in the desired ratios. Morphological analysis revealed spherical TiO2 NCs, rod- and plate-shaped Ag-doped ZnO, and spherical AgO particles. The 50Ti/50ZnAg nanocomposite exhibited superior photocatalytic activity in degrading methylene blue (MB) compared to pure ZnAg and TiO2. In nanotoxicology assessments, except Ag-doped ZnO, the nanocomposites demonstrated good biocompatibility with human peripheral blood mononuclear cells (PBMCs) at concentrations below 100 µg/mL. In Drosophila melanogaster assays, no toxic effects were observed at lower concentrations. Additionally, the nanocomposites effectively inhibited bacterial growth, particularly against antibiotic-resistant strains such as MRSA and KPC, by generating reactive oxygen species (ROS) and disrupting bacterial biofilm formation. Ti/ZnAg nanocomposites exhibited enhanced photocatalytic properties, biocompatibility, and antimicrobial activity, particularly against antibiotic-resistant strains. |
| format | Article |
| id | doaj-art-952f6fddaf2747ff97b4e1551f8fd14e |
| institution | OA Journals |
| issn | 2949-8228 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Next Materials |
| spelling | doaj-art-952f6fddaf2747ff97b4e1551f8fd14e2025-08-20T02:18:48ZengElsevierNext Materials2949-82282025-04-01710044710.1016/j.nxmate.2024.100447Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteriaRafael O. Trevisan0Jerusa M. Oliveira1Hugo F. Perini2Ulisses Travaglini3Thaís K. de L. Rezende4Francisco R.A. dos Santos5Luciana R. de S. Floresta6Ana L.S. Borges7Leticia C. Ruiz8Leonardo E. de A. e Silva9Juliane Z. Marinho10Fernanda M. Fonseca11Carlo J.F. de Oliveira12Virmondes R. Júnior13Marcos V. da Silva14Lucas Anhezini15Anielle Christine A. Silva16Department of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, Brazil; Strategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Laboratory of in vivo Toxicity Analysis and Neurodegenerative Diseases, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, Brazil; Corresponding authors at: Strategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil.Department of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Laboratory of in vivo Toxicity Analysis and Neurodegenerative Diseases, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Laboratory of in vivo Toxicity Analysis and Neurodegenerative Diseases, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, BrazilDepartment of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilDepartment of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, BrazilDepartment of Biomedicine, Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, BrazilDepartment of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilDepartment of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilDepartment of Immunology, Microbiology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, BrazilLaboratory of in vivo Toxicity Analysis and Neurodegenerative Diseases, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, BrazilStrategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil; Rede Nordeste de Biotecnologia (RENORBIO), Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas, Brazil; Corresponding authors at: Strategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Maceió, Alagoas, Brazil.Nanoparticles with bactericidal properties offer significant advantages, and their integration with other nanomaterials can further enhance these benefits. This study investigates the synergistic effects of Ag-doped ZnO/AgO (ZnAg) nanocomposites combined with TiO2 nanocrystals (NCs) as antibacterial agents while assessing their in vitro and in vivo nanotoxicity. Three distinct compositions (25Ti/75ZnAg, 50Ti/50ZnAg, and 75Ti/25ZnAg) were evaluated for their combined effects. Characterization through X-ray diffraction (XRD) and scanning electron microscopy (SEM) confirmed the successful synthesis of nanocomposites with well-dispersed NCs in the desired ratios. Morphological analysis revealed spherical TiO2 NCs, rod- and plate-shaped Ag-doped ZnO, and spherical AgO particles. The 50Ti/50ZnAg nanocomposite exhibited superior photocatalytic activity in degrading methylene blue (MB) compared to pure ZnAg and TiO2. In nanotoxicology assessments, except Ag-doped ZnO, the nanocomposites demonstrated good biocompatibility with human peripheral blood mononuclear cells (PBMCs) at concentrations below 100 µg/mL. In Drosophila melanogaster assays, no toxic effects were observed at lower concentrations. Additionally, the nanocomposites effectively inhibited bacterial growth, particularly against antibiotic-resistant strains such as MRSA and KPC, by generating reactive oxygen species (ROS) and disrupting bacterial biofilm formation. Ti/ZnAg nanocomposites exhibited enhanced photocatalytic properties, biocompatibility, and antimicrobial activity, particularly against antibiotic-resistant strains.http://www.sciencedirect.com/science/article/pii/S2949822824003459Titanium dioxideAg-doped ZnO/AgOMRSAKPCDrosophilaSynergistic |
| spellingShingle | Rafael O. Trevisan Jerusa M. Oliveira Hugo F. Perini Ulisses Travaglini Thaís K. de L. Rezende Francisco R.A. dos Santos Luciana R. de S. Floresta Ana L.S. Borges Leticia C. Ruiz Leonardo E. de A. e Silva Juliane Z. Marinho Fernanda M. Fonseca Carlo J.F. de Oliveira Virmondes R. Júnior Marcos V. da Silva Lucas Anhezini Anielle Christine A. Silva Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria Next Materials Titanium dioxide Ag-doped ZnO/AgO MRSA KPC Drosophila Synergistic |
| title | Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria |
| title_full | Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria |
| title_fullStr | Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria |
| title_full_unstemmed | Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria |
| title_short | Enhanced antibacterial efficacy of biocompatible Ag-doped ZnO/AgO/TiO2 nanocomposites against multiresistant bacteria |
| title_sort | enhanced antibacterial efficacy of biocompatible ag doped zno ago tio2 nanocomposites against multiresistant bacteria |
| topic | Titanium dioxide Ag-doped ZnO/AgO MRSA KPC Drosophila Synergistic |
| url | http://www.sciencedirect.com/science/article/pii/S2949822824003459 |
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