CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment

Dysregulated cell cycle progression is a well-established hallmark of cancer, driving the development of targeted antitumor therapies that intervene at specific phases of the cell cycle. Among these therapeutic targets, cyclin-dependent kinases 4 and 6 (CDK4/6) have emerged as critical regulators of...

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Main Authors: Tong Gao, Ying Sun, Ping Leng, Donghua Liu, Qie Guo, Jing Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1549520/full
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author Tong Gao
Ying Sun
Ping Leng
Donghua Liu
Qie Guo
Jing Li
author_facet Tong Gao
Ying Sun
Ping Leng
Donghua Liu
Qie Guo
Jing Li
author_sort Tong Gao
collection DOAJ
description Dysregulated cell cycle progression is a well-established hallmark of cancer, driving the development of targeted antitumor therapies that intervene at specific phases of the cell cycle. Among these therapeutic targets, cyclin-dependent kinases 4 and 6 (CDK4/6) have emerged as critical regulators of cell cycle progression, with their aberrant activation being strongly implicated in tumorigenesis and cancer progression. Currently, multiple CDK4/6 inhibitors have received clinical approval for hormone receptor (HR)-positive/human epidermal growth factor receptor 2 (HER2)-negative breast cancer, demonstrating dual therapeutic mechanisms through both cell cycle arrest and enhancement of antitumor immunity. However, clinical implementation faces two major challenges: the inevitable development of acquired resistance during prolonged treatment, and the need for optimized combination strategies with other anticancer agents to achieve synergistic efficacy. This review systematically examines the molecular mechanisms underlying CDK4/6 inhibitor function and characterizes currently approved therapeutic agents. Importantly, it synthesizes recent discoveries regarding resistance mechanisms, including dysregulated cell cycle checkpoints, compensatory signaling pathway activation, and tumor microenvironment adaptations. Furthermore, we critically evaluate emerging combination therapeutic approaches targeting these resistance mechanisms. By integrating mechanistic insights with clinical evidence, this analysis aims to provide actionable strategies for overcoming therapeutic resistance and maximizing the clinical potential of CDK4/6 inhibitors in breast cancer management.
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spelling doaj-art-9515436a97bb43d583ec70528cc85d802025-08-20T01:51:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-05-011610.3389/fphar.2025.15495201549520CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatmentTong Gao0Ying Sun1Ping Leng2Donghua Liu3Qie Guo4Jing Li5Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Health Management Center, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDysregulated cell cycle progression is a well-established hallmark of cancer, driving the development of targeted antitumor therapies that intervene at specific phases of the cell cycle. Among these therapeutic targets, cyclin-dependent kinases 4 and 6 (CDK4/6) have emerged as critical regulators of cell cycle progression, with their aberrant activation being strongly implicated in tumorigenesis and cancer progression. Currently, multiple CDK4/6 inhibitors have received clinical approval for hormone receptor (HR)-positive/human epidermal growth factor receptor 2 (HER2)-negative breast cancer, demonstrating dual therapeutic mechanisms through both cell cycle arrest and enhancement of antitumor immunity. However, clinical implementation faces two major challenges: the inevitable development of acquired resistance during prolonged treatment, and the need for optimized combination strategies with other anticancer agents to achieve synergistic efficacy. This review systematically examines the molecular mechanisms underlying CDK4/6 inhibitor function and characterizes currently approved therapeutic agents. Importantly, it synthesizes recent discoveries regarding resistance mechanisms, including dysregulated cell cycle checkpoints, compensatory signaling pathway activation, and tumor microenvironment adaptations. Furthermore, we critically evaluate emerging combination therapeutic approaches targeting these resistance mechanisms. By integrating mechanistic insights with clinical evidence, this analysis aims to provide actionable strategies for overcoming therapeutic resistance and maximizing the clinical potential of CDK4/6 inhibitors in breast cancer management.https://www.frontiersin.org/articles/10.3389/fphar.2025.1549520/fullCDK4/6 inhibitorscell cycle arrestimmune regulationacquired resistancecombination therapy
spellingShingle Tong Gao
Ying Sun
Ping Leng
Donghua Liu
Qie Guo
Jing Li
CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
Frontiers in Pharmacology
CDK4/6 inhibitors
cell cycle arrest
immune regulation
acquired resistance
combination therapy
title CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
title_full CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
title_fullStr CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
title_full_unstemmed CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
title_short CDK4/6 inhibitors in breast cancer therapy: mechanisms of drug resistance and strategies for treatment
title_sort cdk4 6 inhibitors in breast cancer therapy mechanisms of drug resistance and strategies for treatment
topic CDK4/6 inhibitors
cell cycle arrest
immune regulation
acquired resistance
combination therapy
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1549520/full
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