Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies
Preliminary studies of nanoparticles based on the perfluorosulfonic acid resin Nafion have been carried out with the aim of establishing an ionic connection with the protonable phosphine 1,3,5-triaza-7-phosphaadamantane (PTA), suitable for the coordination of platinum. Nafion-containing nanoparticle...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/3206298 |
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| author | Maddalena Sguizzato Elisabetta Esposito Marcus Drechsler Eleonora Gallerani Riccardo Gavioli Paolo Mariani Federica Carducci Rita Cortesi Paola Bergamini |
| author_facet | Maddalena Sguizzato Elisabetta Esposito Marcus Drechsler Eleonora Gallerani Riccardo Gavioli Paolo Mariani Federica Carducci Rita Cortesi Paola Bergamini |
| author_sort | Maddalena Sguizzato |
| collection | DOAJ |
| description | Preliminary studies of nanoparticles based on the perfluorosulfonic acid resin Nafion have been carried out with the aim of establishing an ionic connection with the protonable phosphine 1,3,5-triaza-7-phosphaadamantane (PTA), suitable for the coordination of platinum. Nafion-containing nanoparticles (NAF) were produced by homogenization followed by ultrasonication method. After production, NAF were characterized in terms of size, morphology, and in vitro cytotoxicity. The PCS studies showed that the Z-average mean diameter was around 250 nm. Moreover, the polydispersity index showed a monodimensional distribution of nanoparticles. Cryo-TEM analysis showed a uniform and homogenous population of particles, characterized by the presence of both ovoidal and needle-like structures. To evaluate the in vitro cytotoxicity, NAF were tested on human cancer cell lines K562 and A2780. No cytotoxic effect was found on both cell lines. By 13P-NMR measures, it is here proved that the strongly acidic sulfonic groups of Nafion-containing nanoparticles (NAF) can act as protonating agents for PTA. The protonation occurs selectively at nitrogen; hence protonated PTA maintains its ability to coordinate platinum via its phosphorus atom. |
| format | Article |
| id | doaj-art-9512902ac1534af395f939144a2dbaed |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-9512902ac1534af395f939144a2dbaed2025-02-03T01:01:52ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/32062983206298Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary StudiesMaddalena Sguizzato0Elisabetta Esposito1Marcus Drechsler2Eleonora Gallerani3Riccardo Gavioli4Paolo Mariani5Federica Carducci6Rita Cortesi7Paola Bergamini8Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyBIMF/Soft Matter Electronmicroscopy, University of Bayreuth, Bayreuth, GermanyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, ItalyDepartment of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, ItalyPreliminary studies of nanoparticles based on the perfluorosulfonic acid resin Nafion have been carried out with the aim of establishing an ionic connection with the protonable phosphine 1,3,5-triaza-7-phosphaadamantane (PTA), suitable for the coordination of platinum. Nafion-containing nanoparticles (NAF) were produced by homogenization followed by ultrasonication method. After production, NAF were characterized in terms of size, morphology, and in vitro cytotoxicity. The PCS studies showed that the Z-average mean diameter was around 250 nm. Moreover, the polydispersity index showed a monodimensional distribution of nanoparticles. Cryo-TEM analysis showed a uniform and homogenous population of particles, characterized by the presence of both ovoidal and needle-like structures. To evaluate the in vitro cytotoxicity, NAF were tested on human cancer cell lines K562 and A2780. No cytotoxic effect was found on both cell lines. By 13P-NMR measures, it is here proved that the strongly acidic sulfonic groups of Nafion-containing nanoparticles (NAF) can act as protonating agents for PTA. The protonation occurs selectively at nitrogen; hence protonated PTA maintains its ability to coordinate platinum via its phosphorus atom.http://dx.doi.org/10.1155/2017/3206298 |
| spellingShingle | Maddalena Sguizzato Elisabetta Esposito Marcus Drechsler Eleonora Gallerani Riccardo Gavioli Paolo Mariani Federica Carducci Rita Cortesi Paola Bergamini Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies Journal of Chemistry |
| title | Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies |
| title_full | Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies |
| title_fullStr | Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies |
| title_full_unstemmed | Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies |
| title_short | Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies |
| title_sort | nafion r containing solid lipid nanoparticles as a tool for anticancer pt delivery preliminary studies |
| url | http://dx.doi.org/10.1155/2017/3206298 |
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