Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay
Lateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO<sub>2</sub>@QD@SiO<sub>2</sub> (QD<sup>2</sup>) nano...
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2025-01-01
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| author | Kwanghee Yoo Hye-Seong Cho Jaehi Kim Minsup Shin Jun-Sik Chu Sohyeon Jang Han-Joo Bae Heung Su Jung Homan Kang Bong-Hyun Jun |
| author_facet | Kwanghee Yoo Hye-Seong Cho Jaehi Kim Minsup Shin Jun-Sik Chu Sohyeon Jang Han-Joo Bae Heung Su Jung Homan Kang Bong-Hyun Jun |
| author_sort | Kwanghee Yoo |
| collection | DOAJ |
| description | Lateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO<sub>2</sub>@QD@SiO<sub>2</sub> (QD<sup>2</sup>) nanoparticles, which consist of quantum dots (QDs) embedded in a silica (SiO<sub>2</sub>) core and surrounded by an outer SiO<sub>2</sub> shell, exhibit significantly higher fluorescence intensity (FI) compared to single QDs. In this study, we prepared QD<sup>2</sup>@PEG@Aptamer, an aptamer conjugated with QD<sup>2</sup> using succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol]ester, which is 130 times brighter than single QDs, for detecting carbohydrate antigen (CA) 19-9 through LFIA. For LFIA optimization, we determined the optimal conditions as a 1.0:2.0 × 10<sup>−2</sup> ratio of polyethylene glycol (PEG) to aptamer by adjusting the amounts of PEG and aptamer, phosphate-buffered saline containing 0.5% Tween<sup>®</sup> 20 as a developing solution, and 0.15 μg NPs by setting the NP weight during development. Under these conditions, QD<sup>2</sup>@PEG@Aptamer selectively detected CA19-9, achieving a detection limit of 1.74 × 10<sup>−2</sup> mg·mL<sup>−1</sup>. Moreover, FI remained stable for 10 days after detection. These results highlight the potential of QD<sup>2</sup> and aptamer conjugation technology as a reliable and versatile sensing platform for various diagnostic applications. |
| format | Article |
| id | doaj-art-950c8b29e346426789d8c7d575bfb2a3 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-950c8b29e346426789d8c7d575bfb2a32025-01-24T13:25:34ZengMDPI AGBiosensors2079-63742025-01-011515410.3390/bios15010054Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow ImmunoassayKwanghee Yoo0Hye-Seong Cho1Jaehi Kim2Minsup Shin3Jun-Sik Chu4Sohyeon Jang5Han-Joo Bae6Heung Su Jung7Homan Kang8Bong-Hyun Jun9Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaCompany of Global Zeus, Hwaseong 18363, Republic of KoreaDepartment of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USADepartment of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of KoreaLateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO<sub>2</sub>@QD@SiO<sub>2</sub> (QD<sup>2</sup>) nanoparticles, which consist of quantum dots (QDs) embedded in a silica (SiO<sub>2</sub>) core and surrounded by an outer SiO<sub>2</sub> shell, exhibit significantly higher fluorescence intensity (FI) compared to single QDs. In this study, we prepared QD<sup>2</sup>@PEG@Aptamer, an aptamer conjugated with QD<sup>2</sup> using succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol]ester, which is 130 times brighter than single QDs, for detecting carbohydrate antigen (CA) 19-9 through LFIA. For LFIA optimization, we determined the optimal conditions as a 1.0:2.0 × 10<sup>−2</sup> ratio of polyethylene glycol (PEG) to aptamer by adjusting the amounts of PEG and aptamer, phosphate-buffered saline containing 0.5% Tween<sup>®</sup> 20 as a developing solution, and 0.15 μg NPs by setting the NP weight during development. Under these conditions, QD<sup>2</sup>@PEG@Aptamer selectively detected CA19-9, achieving a detection limit of 1.74 × 10<sup>−2</sup> mg·mL<sup>−1</sup>. Moreover, FI remained stable for 10 days after detection. These results highlight the potential of QD<sup>2</sup> and aptamer conjugation technology as a reliable and versatile sensing platform for various diagnostic applications.https://www.mdpi.com/2079-6374/15/1/54carbohydrate antigen 19-9aptamerquantum dotlateral flow immunoassay |
| spellingShingle | Kwanghee Yoo Hye-Seong Cho Jaehi Kim Minsup Shin Jun-Sik Chu Sohyeon Jang Han-Joo Bae Heung Su Jung Homan Kang Bong-Hyun Jun Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay Biosensors carbohydrate antigen 19-9 aptamer quantum dot lateral flow immunoassay |
| title | Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay |
| title_full | Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay |
| title_fullStr | Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay |
| title_full_unstemmed | Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay |
| title_short | Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay |
| title_sort | aptamer conjugated multi quantum dot embedded silica nanoparticles for lateral flow immunoassay |
| topic | carbohydrate antigen 19-9 aptamer quantum dot lateral flow immunoassay |
| url | https://www.mdpi.com/2079-6374/15/1/54 |
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