Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling
Endophyte can improve plant resistance to Pb stress, and long non-coding RNAs (lncRNAs) have been proved to play a vital role in response to environmental stress. However, there are few studies on the role of lncRNAs induced by endophyte in host plants under Pb stress. Therefore, we conducted high-t...
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325002088 |
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| author | He-Fei Sun Xin-Nan Wang Ya-Nan Li Lan-Lan Wang Yue-Ying Li Lian-Ju Ma Xue-Mei Li |
| author_facet | He-Fei Sun Xin-Nan Wang Ya-Nan Li Lan-Lan Wang Yue-Ying Li Lian-Ju Ma Xue-Mei Li |
| author_sort | He-Fei Sun |
| collection | DOAJ |
| description | Endophyte can improve plant resistance to Pb stress, and long non-coding RNAs (lncRNAs) have been proved to play a vital role in response to environmental stress. However, there are few studies on the role of lncRNAs induced by endophyte in host plants under Pb stress. Therefore, we conducted high-throughput sequencing on root tissue of endophyte-infected and -uninfected rice seedlings under Pb stress, and analyzed the target genes of differentially expressed lncRNAs (DElncRNAs). The results showed that endophyte infection significantly increased plant height, above-ground fresh weight and dry weight, but significantly decreased root length and under-ground dry weight after 5 d of treatment. A total of 413 DElncRNAs (167 down-regulated and 246 up-regulated) were screened. Their target differentially expressed mRNAs (DEmRNAs) were significantly enriched in glutathione metabolism, plasma membrane and mineral elements transfer etc. DEmRNAs were most significantly enriched in glutathione metabolism, thereinto detected total glutathione, reduced and oxidized glutathione contents, glutathione S-transferase and glutathione reductase activities were significantly increased after 5 d of treatment. DElncRNAs and DEmRNAs were combined with miRNA database to construct ceRNA network. DEmRNAs in ceRNA network were mainly participated in carbohydrate metabolic process, peroxidase activity and phenylpropanoid biosynthesis. This study will help to understand the molecular mechanism elicited by endophytic infection within rice seedlings under Pb stress from the perspective of lncRNA. |
| format | Article |
| id | doaj-art-950608d12184437d9be24802f2869f42 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-950608d12184437d9be24802f2869f422025-02-09T04:59:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117872Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedlingHe-Fei Sun0Xin-Nan Wang1Ya-Nan Li2Lan-Lan Wang3Yue-Ying Li4Lian-Ju Ma5Xue-Mei Li6College of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCollege of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCollege of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCollege of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCollege of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCorresponding authors.; College of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaCorresponding authors.; College of Life Science, Shenyang Normal University, No.253 Huanghe North Street, Shenyang 110034, ChinaEndophyte can improve plant resistance to Pb stress, and long non-coding RNAs (lncRNAs) have been proved to play a vital role in response to environmental stress. However, there are few studies on the role of lncRNAs induced by endophyte in host plants under Pb stress. Therefore, we conducted high-throughput sequencing on root tissue of endophyte-infected and -uninfected rice seedlings under Pb stress, and analyzed the target genes of differentially expressed lncRNAs (DElncRNAs). The results showed that endophyte infection significantly increased plant height, above-ground fresh weight and dry weight, but significantly decreased root length and under-ground dry weight after 5 d of treatment. A total of 413 DElncRNAs (167 down-regulated and 246 up-regulated) were screened. Their target differentially expressed mRNAs (DEmRNAs) were significantly enriched in glutathione metabolism, plasma membrane and mineral elements transfer etc. DEmRNAs were most significantly enriched in glutathione metabolism, thereinto detected total glutathione, reduced and oxidized glutathione contents, glutathione S-transferase and glutathione reductase activities were significantly increased after 5 d of treatment. DElncRNAs and DEmRNAs were combined with miRNA database to construct ceRNA network. DEmRNAs in ceRNA network were mainly participated in carbohydrate metabolic process, peroxidase activity and phenylpropanoid biosynthesis. This study will help to understand the molecular mechanism elicited by endophytic infection within rice seedlings under Pb stress from the perspective of lncRNA.http://www.sciencedirect.com/science/article/pii/S0147651325002088LncRNAPb stressEndophyteGlutathione metabolism |
| spellingShingle | He-Fei Sun Xin-Nan Wang Ya-Nan Li Lan-Lan Wang Yue-Ying Li Lian-Ju Ma Xue-Mei Li Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling Ecotoxicology and Environmental Safety LncRNA Pb stress Endophyte Glutathione metabolism |
| title | Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling |
| title_full | Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling |
| title_fullStr | Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling |
| title_full_unstemmed | Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling |
| title_short | Long non-coding RNAs modulate glutathione metabolism gene expression and tolerance to Pb stress in root tissue of endophyte-infected rice seedling |
| title_sort | long non coding rnas modulate glutathione metabolism gene expression and tolerance to pb stress in root tissue of endophyte infected rice seedling |
| topic | LncRNA Pb stress Endophyte Glutathione metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325002088 |
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