Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation

IntroductionAllyl isothiocyanate (AITC) has demonstrated efficacy as a soil fumigant, effectively controlling soil-borne pathogens and nematodes. Although AITC has a significant effect on soil microbial communities, whether fumigation affects the production of crop endophytes is unclear.MethodsIn th...

Full description

Saved in:
Bibliographic Details
Main Authors: Wenfeng Tian, Qiuxia Wang, Aocheng Cao, Dongdong Yan, Yuan Li, Zhoubin Liu, Bozhi Yang, Wensheng Fang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1520443/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849726405795905536
author Wenfeng Tian
Wenfeng Tian
Qiuxia Wang
Aocheng Cao
Dongdong Yan
Yuan Li
Zhoubin Liu
Bozhi Yang
Wensheng Fang
author_facet Wenfeng Tian
Wenfeng Tian
Qiuxia Wang
Aocheng Cao
Dongdong Yan
Yuan Li
Zhoubin Liu
Bozhi Yang
Wensheng Fang
author_sort Wenfeng Tian
collection DOAJ
description IntroductionAllyl isothiocyanate (AITC) has demonstrated efficacy as a soil fumigant, effectively controlling soil-borne pathogens and nematodes. Although AITC has a significant effect on soil microbial communities, whether fumigation affects the production of crop endophytes is unclear.MethodsIn this study, AITC was used to fumigate the soil, and the response of endophytic bacteria (in roots, stems, and leaves) in different pepper genotypes (Xiangla359, La Xuan, Shuang Jiao) was investigated.ResultsFumigation with AITC significantly increased soil microbial diversity, stimulated the growth of Actinomycetota, and inhibited Pseudomonadota. However, the effects on endophytic bacteria varied among pepper varieties. Specifically, fumigation significantly reduced microbial diversity in the roots and leaves of Xiangla359, but had no significant effect on La Xuan and Shuang Jiao. Furthermore, the growth-promoting effect of AITC was most pronounced in Xiangla359.ConclusionOur results suggest that while AITC fumigation significantly alters soil microbial diversity and composition, its effects on crop endophytes are genotype-dependent. These findings provide insight into the complex interactions between soil microbial communities and crop endophytes in response to soil fumigation.
format Article
id doaj-art-94fa1aefefef4a2f80911a7f63e0d9ba
institution DOAJ
issn 1664-302X
language English
publishDate 2025-05-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Microbiology
spelling doaj-art-94fa1aefefef4a2f80911a7f63e0d9ba2025-08-20T03:10:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15204431520443Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigationWenfeng Tian0Wenfeng Tian1Qiuxia Wang2Aocheng Cao3Dongdong Yan4Yuan Li5Zhoubin Liu6Bozhi Yang7Wensheng Fang8Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaEngineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaEngineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaIntroductionAllyl isothiocyanate (AITC) has demonstrated efficacy as a soil fumigant, effectively controlling soil-borne pathogens and nematodes. Although AITC has a significant effect on soil microbial communities, whether fumigation affects the production of crop endophytes is unclear.MethodsIn this study, AITC was used to fumigate the soil, and the response of endophytic bacteria (in roots, stems, and leaves) in different pepper genotypes (Xiangla359, La Xuan, Shuang Jiao) was investigated.ResultsFumigation with AITC significantly increased soil microbial diversity, stimulated the growth of Actinomycetota, and inhibited Pseudomonadota. However, the effects on endophytic bacteria varied among pepper varieties. Specifically, fumigation significantly reduced microbial diversity in the roots and leaves of Xiangla359, but had no significant effect on La Xuan and Shuang Jiao. Furthermore, the growth-promoting effect of AITC was most pronounced in Xiangla359.ConclusionOur results suggest that while AITC fumigation significantly alters soil microbial diversity and composition, its effects on crop endophytes are genotype-dependent. These findings provide insight into the complex interactions between soil microbial communities and crop endophytes in response to soil fumigation.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1520443/fullsoil fumigationendophytesoil microorganismAITCgenotypepepper
spellingShingle Wenfeng Tian
Wenfeng Tian
Qiuxia Wang
Aocheng Cao
Dongdong Yan
Yuan Li
Zhoubin Liu
Bozhi Yang
Wensheng Fang
Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
Frontiers in Microbiology
soil fumigation
endophyte
soil microorganism
AITC
genotype
pepper
title Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
title_full Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
title_fullStr Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
title_full_unstemmed Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
title_short Genotype-dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
title_sort genotype dependent responses of pepper endophytes to soil microbial community shifts induced by allyl isothiocyanate fumigation
topic soil fumigation
endophyte
soil microorganism
AITC
genotype
pepper
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1520443/full
work_keys_str_mv AT wenfengtian genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT wenfengtian genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT qiuxiawang genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT aochengcao genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT dongdongyan genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT yuanli genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT zhoubinliu genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT bozhiyang genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation
AT wenshengfang genotypedependentresponsesofpepperendophytestosoilmicrobialcommunityshiftsinducedbyallylisothiocyanatefumigation