Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass

ABSTRACT This study aimed to evaluate the effects of supplementation of live yeast (LY) associated with starch levels (SL) on the in vitro kinetic parameters of the gas production (GP), in vitro neutral detergent fiber digestibility (IVNDFD), volatile fatty acid (VFA) and methane (CH4) production of...

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Main Authors: Elder Cavalcante Fabian, Bruno Carneiro e Pedreira, Ana Cláudia da Costa, Nelcino Francisco de Paula, Alessandra Schaphauser Rosseto Fonseca, Karine Padilha Nunes Vieira, Rodrigo Dias Lauritano Pacheco, José Roberto Tavares, Farah Arruda Petter, Fagton de Mattos Negrão, Luciano da Silva Cabral
Format: Article
Language:English
Published: Universidade de São Paulo 2025-03-01
Series:Scientia Agricola
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162025000100403&lng=en&tlng=en
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author Elder Cavalcante Fabian
Bruno Carneiro e Pedreira
Ana Cláudia da Costa
Nelcino Francisco de Paula
Alessandra Schaphauser Rosseto Fonseca
Karine Padilha Nunes Vieira
Rodrigo Dias Lauritano Pacheco
José Roberto Tavares
Farah Arruda Petter
Fagton de Mattos Negrão
Luciano da Silva Cabral
author_facet Elder Cavalcante Fabian
Bruno Carneiro e Pedreira
Ana Cláudia da Costa
Nelcino Francisco de Paula
Alessandra Schaphauser Rosseto Fonseca
Karine Padilha Nunes Vieira
Rodrigo Dias Lauritano Pacheco
José Roberto Tavares
Farah Arruda Petter
Fagton de Mattos Negrão
Luciano da Silva Cabral
author_sort Elder Cavalcante Fabian
collection DOAJ
description ABSTRACT This study aimed to evaluate the effects of supplementation of live yeast (LY) associated with starch levels (SL) on the in vitro kinetic parameters of the gas production (GP), in vitro neutral detergent fiber digestibility (IVNDFD), volatile fatty acid (VFA) and methane (CH4) production of Urochloa brizantha cv. Marandu grass harvested in both the rainy and dry seasons. The study was conducted in a 2 × 3 factorial arrangement, whereby LY supplementation (with and without LY) was associated with 0, 10, and 20 % of starch supplementation. The variables were analyzed using PROC MIXED in SAS (Statistical Analysis System, version 9.3). LY and SL had no interaction effects on any of the variables tested. LY supplementation increased total GP at 72 h of incubation in both forage seasons by approximately 25 %. Additionally, LY supplementation decreased lag time; however, the GP rate was unaffected. The LY also caused a slight drop in IVNDFD at 48 h in both forage seasons. LY supplementation decreased total VFA production by 25.96 % for the rainy season and 11.32 % for the dry season and caused a 40 and 46 % drop in CH4 for the rainy and dry season forage, respectively. The assumption that combining LY and starch would enhance LY activity was not confirmed in this study, as most analyzed variables showed no interaction between the two factors. Consequently, no beneficial effects on increased SL could be attributed to LY supplementation.
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spelling doaj-art-94f92d523d7f4cc99fb48ddd3fe08e162025-08-20T03:01:50ZengUniversidade de São PauloScientia Agricola1678-992X2025-03-018210.1590/1678-992x-2024-0059Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grassElder Cavalcante Fabianhttps://orcid.org/0009-0001-7592-5580Bruno Carneiro e Pedreirahttps://orcid.org/0000-0003-4663-954XAna Cláudia da Costahttps://orcid.org/0000-0001-7764-112XNelcino Francisco de Paulahttps://orcid.org/0000-0002-0392-5653Alessandra Schaphauser Rosseto Fonsecahttps://orcid.org/0000-0003-3199-8478Karine Padilha Nunes Vieirahttps://orcid.org/0000-0002-6882-7202Rodrigo Dias Lauritano Pachecohttps://orcid.org/0000-0003-2307-1429José Roberto Tavareshttps://orcid.org/0000-0001-6856-3502Farah Arruda Petterhttps://orcid.org/0000-0003-2545-8889Fagton de Mattos Negrãohttps://orcid.org/0000-0002-2134-0974Luciano da Silva Cabralhttps://orcid.org/0000-0001-6385-4618ABSTRACT This study aimed to evaluate the effects of supplementation of live yeast (LY) associated with starch levels (SL) on the in vitro kinetic parameters of the gas production (GP), in vitro neutral detergent fiber digestibility (IVNDFD), volatile fatty acid (VFA) and methane (CH4) production of Urochloa brizantha cv. Marandu grass harvested in both the rainy and dry seasons. The study was conducted in a 2 × 3 factorial arrangement, whereby LY supplementation (with and without LY) was associated with 0, 10, and 20 % of starch supplementation. The variables were analyzed using PROC MIXED in SAS (Statistical Analysis System, version 9.3). LY and SL had no interaction effects on any of the variables tested. LY supplementation increased total GP at 72 h of incubation in both forage seasons by approximately 25 %. Additionally, LY supplementation decreased lag time; however, the GP rate was unaffected. The LY also caused a slight drop in IVNDFD at 48 h in both forage seasons. LY supplementation decreased total VFA production by 25.96 % for the rainy season and 11.32 % for the dry season and caused a 40 and 46 % drop in CH4 for the rainy and dry season forage, respectively. The assumption that combining LY and starch would enhance LY activity was not confirmed in this study, as most analyzed variables showed no interaction between the two factors. Consequently, no beneficial effects on increased SL could be attributed to LY supplementation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162025000100403&lng=en&tlng=enVFAdigestibilitygas productionprobiotic
spellingShingle Elder Cavalcante Fabian
Bruno Carneiro e Pedreira
Ana Cláudia da Costa
Nelcino Francisco de Paula
Alessandra Schaphauser Rosseto Fonseca
Karine Padilha Nunes Vieira
Rodrigo Dias Lauritano Pacheco
José Roberto Tavares
Farah Arruda Petter
Fagton de Mattos Negrão
Luciano da Silva Cabral
Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
Scientia Agricola
VFA
digestibility
gas production
probiotic
title Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
title_full Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
title_fullStr Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
title_full_unstemmed Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
title_short Effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
title_sort effects of live yeast and starch supplementation on in vitro fermentation and methane production of tropical grass
topic VFA
digestibility
gas production
probiotic
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162025000100403&lng=en&tlng=en
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