Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control
The transport of intensity equation enables quantitative phase imaging from only two axially displaced intensity images, facilitating the characterization of low-contrast samples like cells and microorganisms. However, the rapid selection of the correct defocused planes, crucial for real-time phase...
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| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/14/4503 |
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| author | Christos Alexandropoulos Laura Rodríguez-Suñé Martí Duocastella |
| author_facet | Christos Alexandropoulos Laura Rodríguez-Suñé Martí Duocastella |
| author_sort | Christos Alexandropoulos |
| collection | DOAJ |
| description | The transport of intensity equation enables quantitative phase imaging from only two axially displaced intensity images, facilitating the characterization of low-contrast samples like cells and microorganisms. However, the rapid selection of the correct defocused planes, crucial for real-time phase imaging of dynamic events, remains challenging. Additionally, the different images are normally acquired sequentially, further limiting phase-reconstruction speed. Here, we report on a system that addresses these issues and enables user-tuned defocusing with snapshot phase retrieval. Our approach is based on combining multi-color pulsed illumination with acousto-optic defocusing for microsecond-scale chromatic aberration control. By illuminating each plane with a different color and using a color camera, the information to reconstruct a phase map can be gathered in a single acquisition. We detail the fundamentals of our method, characterize its performance, and demonstrate live phase imaging of a freely moving microorganism at speeds of 150 phase reconstructions per second, limited only by the camera’s frame rate. |
| format | Article |
| id | doaj-art-94eebc5f436a4b09afb0641d2cc3d995 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-94eebc5f436a4b09afb0641d2cc3d9952025-08-20T03:32:15ZengMDPI AGSensors1424-82202025-07-012514450310.3390/s25144503Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration ControlChristos Alexandropoulos0Laura Rodríguez-Suñé1Martí Duocastella2Department of Applied Physics, Universitat de Barcelona, C/Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Applied Physics, Universitat de Barcelona, C/Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Applied Physics, Universitat de Barcelona, C/Martí i Franquès 1, 08028 Barcelona, SpainThe transport of intensity equation enables quantitative phase imaging from only two axially displaced intensity images, facilitating the characterization of low-contrast samples like cells and microorganisms. However, the rapid selection of the correct defocused planes, crucial for real-time phase imaging of dynamic events, remains challenging. Additionally, the different images are normally acquired sequentially, further limiting phase-reconstruction speed. Here, we report on a system that addresses these issues and enables user-tuned defocusing with snapshot phase retrieval. Our approach is based on combining multi-color pulsed illumination with acousto-optic defocusing for microsecond-scale chromatic aberration control. By illuminating each plane with a different color and using a color camera, the information to reconstruct a phase map can be gathered in a single acquisition. We detail the fundamentals of our method, characterize its performance, and demonstrate live phase imaging of a freely moving microorganism at speeds of 150 phase reconstructions per second, limited only by the camera’s frame rate.https://www.mdpi.com/1424-8220/25/14/4503label-free microscopyacousto-opticstransport of intensity equationliquid lensillumination encoding |
| spellingShingle | Christos Alexandropoulos Laura Rodríguez-Suñé Martí Duocastella Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control Sensors label-free microscopy acousto-optics transport of intensity equation liquid lens illumination encoding |
| title | Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control |
| title_full | Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control |
| title_fullStr | Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control |
| title_full_unstemmed | Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control |
| title_short | Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control |
| title_sort | snapshot quantitative phase imaging with acousto optic chromatic aberration control |
| topic | label-free microscopy acousto-optics transport of intensity equation liquid lens illumination encoding |
| url | https://www.mdpi.com/1424-8220/25/14/4503 |
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