Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer
BackgroundPhosducin-like 3 (PDCL3), a member of the photoreceptor family, is involved in angiogenesis and apoptosis. However, there is no pan-cancer analysis, and few studies have explored the effect of PDCL3 on tumor immune infiltration.MethodPublic datasets were used to explore the diagnostic and...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1604179/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849427990645047296 |
|---|---|
| author | Zihao Li Jiayi Li Jiayi Li Fengchang Li Honghua Liang Zuotao Wu Yongjie Zhu Jusen Nong Ting Zhuo Peng Luo Lingyun He Weijia Huang Jianbin Cao |
| author_facet | Zihao Li Jiayi Li Jiayi Li Fengchang Li Honghua Liang Zuotao Wu Yongjie Zhu Jusen Nong Ting Zhuo Peng Luo Lingyun He Weijia Huang Jianbin Cao |
| author_sort | Zihao Li |
| collection | DOAJ |
| description | BackgroundPhosducin-like 3 (PDCL3), a member of the photoreceptor family, is involved in angiogenesis and apoptosis. However, there is no pan-cancer analysis, and few studies have explored the effect of PDCL3 on tumor immune infiltration.MethodPublic datasets were used to explore the diagnostic and prognostic value of PDCL3. The relationship between PDCL3 expression and immune infiltration, tumor mutation burden (TMB), and microsatellite instability (MSI) was investigated. Additionally, the therapeutic value of PDCL3 was explored. Finally, differences in PDCL3 expression across cell clusters were analyzed using single-cell datasets. In vitro cellular assays were performed to assess the impact of PDCL3 expression on the proliferative capacity, migratory potential, and invasive properties of non-small cell lung cancer (NSCLC) cells.ResultsPDCL3 expression was upregulated in most tumors and correlated with poor outcomes, showing diagnostic and prognostic value. In addition, PDCL3 expression exhibited a positive correlation with infiltration of T helper 2 (Th2) cells and a negative correlation with infiltration of plasmacytoid dendritic cells (pDCs) across a variety of tumors. A relationship was also found between PDCL3 expression and TMB and MSI. Single-cell dataset analysis confirmed that PDCL3 expression was primarily in cancer cells and macrophages. In vitro functional analyses demonstrated that genetic silencing of PDCL3 significantly reduced proliferative rates, migratory activity, and invasive potential in pulmonary carcinoma cell models.ConclusionsPDCL3 may contribute to cancer progression and is a potential candidate biomarker for pan-cancer diagnosis and prognosis. These findings suggest that targeting PDCL3 may provide a valuable strategy for cancer immunotherapy. |
| format | Article |
| id | doaj-art-94e9e575e76647feb7fffd399c34b756 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-94e9e575e76647feb7fffd399c34b7562025-08-20T03:28:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-07-011610.3389/fimmu.2025.16041791604179Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancerZihao Li0Jiayi Li1Jiayi Li2Fengchang Li3Honghua Liang4Zuotao Wu5Yongjie Zhu6Jusen Nong7Ting Zhuo8Peng Luo9Lingyun He10Weijia Huang11Jianbin Cao12Department of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Nephrology, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Cardio-Thoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Cardio-Thoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Pediatric Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Thoracic Surgery, Liuzhou People’s Hospital affiliated to Guangxi Medical University, Liuzhou, Guangxi, ChinaBackgroundPhosducin-like 3 (PDCL3), a member of the photoreceptor family, is involved in angiogenesis and apoptosis. However, there is no pan-cancer analysis, and few studies have explored the effect of PDCL3 on tumor immune infiltration.MethodPublic datasets were used to explore the diagnostic and prognostic value of PDCL3. The relationship between PDCL3 expression and immune infiltration, tumor mutation burden (TMB), and microsatellite instability (MSI) was investigated. Additionally, the therapeutic value of PDCL3 was explored. Finally, differences in PDCL3 expression across cell clusters were analyzed using single-cell datasets. In vitro cellular assays were performed to assess the impact of PDCL3 expression on the proliferative capacity, migratory potential, and invasive properties of non-small cell lung cancer (NSCLC) cells.ResultsPDCL3 expression was upregulated in most tumors and correlated with poor outcomes, showing diagnostic and prognostic value. In addition, PDCL3 expression exhibited a positive correlation with infiltration of T helper 2 (Th2) cells and a negative correlation with infiltration of plasmacytoid dendritic cells (pDCs) across a variety of tumors. A relationship was also found between PDCL3 expression and TMB and MSI. Single-cell dataset analysis confirmed that PDCL3 expression was primarily in cancer cells and macrophages. In vitro functional analyses demonstrated that genetic silencing of PDCL3 significantly reduced proliferative rates, migratory activity, and invasive potential in pulmonary carcinoma cell models.ConclusionsPDCL3 may contribute to cancer progression and is a potential candidate biomarker for pan-cancer diagnosis and prognosis. These findings suggest that targeting PDCL3 may provide a valuable strategy for cancer immunotherapy.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1604179/fullPDCL3pan-cancerbiomarkerbioinformatics analysisimmune infiltration |
| spellingShingle | Zihao Li Jiayi Li Jiayi Li Fengchang Li Honghua Liang Zuotao Wu Yongjie Zhu Jusen Nong Ting Zhuo Peng Luo Lingyun He Weijia Huang Jianbin Cao Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer Frontiers in Immunology PDCL3 pan-cancer biomarker bioinformatics analysis immune infiltration |
| title | Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer |
| title_full | Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer |
| title_fullStr | Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer |
| title_full_unstemmed | Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer |
| title_short | Comprehensive analysis of phosducin-like 3 as a diagnostic, prognostic and immunological marker in pan-cancer |
| title_sort | comprehensive analysis of phosducin like 3 as a diagnostic prognostic and immunological marker in pan cancer |
| topic | PDCL3 pan-cancer biomarker bioinformatics analysis immune infiltration |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1604179/full |
| work_keys_str_mv | AT zihaoli comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT jiayili comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT jiayili comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT fengchangli comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT honghualiang comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT zuotaowu comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT yongjiezhu comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT jusennong comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT tingzhuo comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT pengluo comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT lingyunhe comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT weijiahuang comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer AT jianbincao comprehensiveanalysisofphosducinlike3asadiagnosticprognosticandimmunologicalmarkerinpancancer |