Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities

Abstract The gut microbiota plays a crucial role in gastrointestinal health, immune function, and overall well-being. Dysbiosis has been linked to various conditions such as colon cancer, atopic diseases, mental disorders, autoimmune disorders, obesity, and diabetes. This in vitro study aims to asse...

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Main Authors: Asmaa Negm El-Dein, Wafa A. Alshehri, Ashjan F. Khalel, Hassan M. Awad
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Microbiology
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Online Access:https://doi.org/10.1186/s12866-025-04062-9
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author Asmaa Negm El-Dein
Wafa A. Alshehri
Ashjan F. Khalel
Hassan M. Awad
author_facet Asmaa Negm El-Dein
Wafa A. Alshehri
Ashjan F. Khalel
Hassan M. Awad
author_sort Asmaa Negm El-Dein
collection DOAJ
description Abstract The gut microbiota plays a crucial role in gastrointestinal health, immune function, and overall well-being. Dysbiosis has been linked to various conditions such as colon cancer, atopic diseases, mental disorders, autoimmune disorders, obesity, and diabetes. This in vitro study aims to assess the safety and functional potential of two probiotic strains, Lactiplantibacillus (L) plantarum and Bifidobacterium (B) longum, focusing on their anti-lipidemic, anti-diabetic, antioxidant, and probiotic properties. The strains were tested for stress tolerance, including acidic, alkaline, osmotic, oxidative, thermal, detergent, bile salt, and pancreatic enzyme conditions. Both strains exhibited strong resilience, often surpassing the control strain. Their antioxidant activity, measured by radical scavenging ability, was comparable to ascorbic acid, with values of 77% for L. plantarum and 92% for B. longum. Cholesterol-lowering capacity reached 50% and 49% after 3 days, increasing to 59% and 78% after 7 days, respectively. Hydrophobicity, an indicator of adhesion potential, was approximately 78% for L. plantarum and 80% for B. longum. Additionally, both strains showed low α-amylase activity (91.65 and 92.33 U/ml), suggesting a potential role in slowing carbohydrate digestion and managing blood glucose levels. Overall, the strains demonstrated favorable safety profiles and promising functional attributes for alleviating hyperlipidemia and diabetes. PCA and heatmap analyses further highlighted L. plantarum as the most promising candidate.
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spelling doaj-art-94e04d99d515411bb3100257b7b02e372025-08-20T02:05:49ZengBMCBMC Microbiology1471-21802025-06-0125111110.1186/s12866-025-04062-9Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activitiesAsmaa Negm El-Dein0Wafa A. Alshehri1Ashjan F. Khalel2Hassan M. Awad3Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreDepartment of Biological Sciences, College of Science, University of JeddahBiology Department, University Collage of Aldarb, Jazan UniversityChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreAbstract The gut microbiota plays a crucial role in gastrointestinal health, immune function, and overall well-being. Dysbiosis has been linked to various conditions such as colon cancer, atopic diseases, mental disorders, autoimmune disorders, obesity, and diabetes. This in vitro study aims to assess the safety and functional potential of two probiotic strains, Lactiplantibacillus (L) plantarum and Bifidobacterium (B) longum, focusing on their anti-lipidemic, anti-diabetic, antioxidant, and probiotic properties. The strains were tested for stress tolerance, including acidic, alkaline, osmotic, oxidative, thermal, detergent, bile salt, and pancreatic enzyme conditions. Both strains exhibited strong resilience, often surpassing the control strain. Their antioxidant activity, measured by radical scavenging ability, was comparable to ascorbic acid, with values of 77% for L. plantarum and 92% for B. longum. Cholesterol-lowering capacity reached 50% and 49% after 3 days, increasing to 59% and 78% after 7 days, respectively. Hydrophobicity, an indicator of adhesion potential, was approximately 78% for L. plantarum and 80% for B. longum. Additionally, both strains showed low α-amylase activity (91.65 and 92.33 U/ml), suggesting a potential role in slowing carbohydrate digestion and managing blood glucose levels. Overall, the strains demonstrated favorable safety profiles and promising functional attributes for alleviating hyperlipidemia and diabetes. PCA and heatmap analyses further highlighted L. plantarum as the most promising candidate.https://doi.org/10.1186/s12866-025-04062-9Lactiplantibacillus plantarumBifidobacterium longumGenotypic identificationProbiotic propertiesIn vitro anti-lipidemic and anti-diabetic activities
spellingShingle Asmaa Negm El-Dein
Wafa A. Alshehri
Ashjan F. Khalel
Hassan M. Awad
Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
BMC Microbiology
Lactiplantibacillus plantarum
Bifidobacterium longum
Genotypic identification
Probiotic properties
In vitro anti-lipidemic and anti-diabetic activities
title Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
title_full Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
title_fullStr Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
title_full_unstemmed Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
title_short Comparative in silico analyses between Lactiplantibacillus plantarum and Bifidobacterium longum concerning probiotic properties, anti-lipidemic, and anti-diabetic in vitro activities
title_sort comparative in silico analyses between lactiplantibacillus plantarum and bifidobacterium longum concerning probiotic properties anti lipidemic and anti diabetic in vitro activities
topic Lactiplantibacillus plantarum
Bifidobacterium longum
Genotypic identification
Probiotic properties
In vitro anti-lipidemic and anti-diabetic activities
url https://doi.org/10.1186/s12866-025-04062-9
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