Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model
Background Accumulating evidence demonstrates that an increased tumor-associated macrophage abundance is often associated with poor prognosis in colorectal cancer (CRC). The mechanism underlying the effect of tumor-derived exosomes on M2 macrophage polarization remains elusive.Results The novel circ...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2024-05-01
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| Series: | Journal for ImmunoTherapy of Cancer |
| Online Access: | https://jitc.bmj.com/content/12/5/e008491.full |
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| author | Yang Liu Chen Chen Jinbo Liu Zhenqiang Sun Bo Shao Yanxin Xu Pengyuan Dang Shengyun Hu Haifeng Sun Chaoguan Wang Junhong Hu |
| author_facet | Yang Liu Chen Chen Jinbo Liu Zhenqiang Sun Bo Shao Yanxin Xu Pengyuan Dang Shengyun Hu Haifeng Sun Chaoguan Wang Junhong Hu |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Background Accumulating evidence demonstrates that an increased tumor-associated macrophage abundance is often associated with poor prognosis in colorectal cancer (CRC). The mechanism underlying the effect of tumor-derived exosomes on M2 macrophage polarization remains elusive.Results The novel circular RNA circPOLQ exhibited significantly higher expression in CRC tissues than in paired normal tissues. Higher circPOLQ expression was associated with poorer prognosis in patients with CRC. In vitro and in vivo experiments showed that tumor-derived exosomal circPOLQ did not directly regulate CRC cell development but promoted CRC metastatic nodule formation by enhancing M2 macrophage polarization. circPOLQ activated the interleukin-10/signal transducer and activator of transcription 3 axis by targeting miR-379–3 p to promote M2 macrophage polarization.Conclusion circPOLQ can enter macrophages via CRC cell-derived exosomes and promote CRC metastatic nodule formation by enhancing M2 macrophage polarization. These findings reveal a tumor-derived exosome-mediated tumor–macrophage interaction potentially affecting CRC metastatic nodule formation. |
| format | Article |
| id | doaj-art-94cfc39da1df4f1a8f47058d40b421e7 |
| institution | Kabale University |
| issn | 2051-1426 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Journal for ImmunoTherapy of Cancer |
| spelling | doaj-art-94cfc39da1df4f1a8f47058d40b421e72025-02-03T17:30:11ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262024-05-0112510.1136/jitc-2023-008491Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer modelYang Liu0Chen Chen1Jinbo Liu2Zhenqiang Sun3Bo Shao4Yanxin Xu5Pengyuan Dang6Shengyun Hu7Haifeng Sun8Chaoguan Wang9Junhong Hu10Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi`an, ChinaDepartment of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Radiotherapy, Henan Cancer Hospital Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaBackground Accumulating evidence demonstrates that an increased tumor-associated macrophage abundance is often associated with poor prognosis in colorectal cancer (CRC). The mechanism underlying the effect of tumor-derived exosomes on M2 macrophage polarization remains elusive.Results The novel circular RNA circPOLQ exhibited significantly higher expression in CRC tissues than in paired normal tissues. Higher circPOLQ expression was associated with poorer prognosis in patients with CRC. In vitro and in vivo experiments showed that tumor-derived exosomal circPOLQ did not directly regulate CRC cell development but promoted CRC metastatic nodule formation by enhancing M2 macrophage polarization. circPOLQ activated the interleukin-10/signal transducer and activator of transcription 3 axis by targeting miR-379–3 p to promote M2 macrophage polarization.Conclusion circPOLQ can enter macrophages via CRC cell-derived exosomes and promote CRC metastatic nodule formation by enhancing M2 macrophage polarization. These findings reveal a tumor-derived exosome-mediated tumor–macrophage interaction potentially affecting CRC metastatic nodule formation.https://jitc.bmj.com/content/12/5/e008491.full |
| spellingShingle | Yang Liu Chen Chen Jinbo Liu Zhenqiang Sun Bo Shao Yanxin Xu Pengyuan Dang Shengyun Hu Haifeng Sun Chaoguan Wang Junhong Hu Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model Journal for ImmunoTherapy of Cancer |
| title | Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model |
| title_full | Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model |
| title_fullStr | Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model |
| title_full_unstemmed | Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model |
| title_short | Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model |
| title_sort | exosomal circpolq promotes macrophage m2 polarization via activating il 10 stat3 axis in a colorectal cancer model |
| url | https://jitc.bmj.com/content/12/5/e008491.full |
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