Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study
Background and objectives. SDF and fluoride varnish have garnered attention for their ease of application and potential to address ECC, particularly in young children with limited cooperative abilities for traditional dental procedures. But the cytotoxicity of these materials are not yet studied ext...
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| Format: | Article |
| Language: | English |
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Amaltea Medical Publishing House
2024-06-01
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| Series: | Romanian Journal of Stomatology |
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| Online Access: | https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-13.pdf |
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| author | Manisha Bala Rathy Mahesh Ramakrishnan |
| author_facet | Manisha Bala Rathy Mahesh Ramakrishnan |
| author_sort | Manisha Bala Rathy |
| collection | DOAJ |
| description | Background and objectives. SDF and fluoride varnish have garnered attention for their ease of application and potential to address ECC, particularly in young children with limited cooperative abilities for traditional dental procedures. But the cytotoxicity of these materials are not yet studied extensively. Materials and methods. Extracted human primary molars of comparable size and with no visible cavitated carious lesions were collected. The teeth were stored for less than 30 days in 0.1% thymol solution at 23°C prior to the experiment. Each tooth was coated with fluoride varnish and SDF respectively. MTT assay was performed and cytotoxicity is evaluated. Results. The materials used in this study - SDF and fluoride varnish showed significantly lower cell viability compared to the control group. There was significantly higher cell viability in SDF samples compared to fluoride varnish. Conclusions. Within the limitations of the study, it is concluded that the cell viability of SDF is higher when compared with the fluoride varnish. |
| format | Article |
| id | doaj-art-94c4c404bfbf47d9ada241be59290291 |
| institution | Kabale University |
| issn | 1843-0805 2069-6078 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Stomatology |
| spelling | doaj-art-94c4c404bfbf47d9ada241be592902912025-01-08T09:20:19ZengAmaltea Medical Publishing HouseRomanian Journal of Stomatology1843-08052069-60782024-06-0170214815210.37897/RJS.2024.2.13Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro studyManisha Bala Rathy0Mahesh Ramakrishnan1Department of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals; Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaDepartment of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals; Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaBackground and objectives. SDF and fluoride varnish have garnered attention for their ease of application and potential to address ECC, particularly in young children with limited cooperative abilities for traditional dental procedures. But the cytotoxicity of these materials are not yet studied extensively. Materials and methods. Extracted human primary molars of comparable size and with no visible cavitated carious lesions were collected. The teeth were stored for less than 30 days in 0.1% thymol solution at 23°C prior to the experiment. Each tooth was coated with fluoride varnish and SDF respectively. MTT assay was performed and cytotoxicity is evaluated. Results. The materials used in this study - SDF and fluoride varnish showed significantly lower cell viability compared to the control group. There was significantly higher cell viability in SDF samples compared to fluoride varnish. Conclusions. Within the limitations of the study, it is concluded that the cell viability of SDF is higher when compared with the fluoride varnish.https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-13.pdfcytotoxicityfluoride varnishsdfmtt assay |
| spellingShingle | Manisha Bala Rathy Mahesh Ramakrishnan Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study Romanian Journal of Stomatology cytotoxicity fluoride varnish sdf mtt assay |
| title | Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study |
| title_full | Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study |
| title_fullStr | Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study |
| title_full_unstemmed | Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study |
| title_short | Cytotoxicity comparison between fluoride varnish and SDF in pulpal stem cells using MTT assay: an in vitro study |
| title_sort | cytotoxicity comparison between fluoride varnish and sdf in pulpal stem cells using mtt assay an in vitro study |
| topic | cytotoxicity fluoride varnish sdf mtt assay |
| url | https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-13.pdf |
| work_keys_str_mv | AT manishabalarathy cytotoxicitycomparisonbetweenfluoridevarnishandsdfinpulpalstemcellsusingmttassayaninvitrostudy AT maheshramakrishnan cytotoxicitycomparisonbetweenfluoridevarnishandsdfinpulpalstemcellsusingmttassayaninvitrostudy |