In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond
<i>Ichthyophthirius multifiliis</i>, a parasitic ciliate, causes “white spot disease” in freshwater fish and poses a significant threat to global freshwater aquaculture. Eliminating the free-swimming theront stage from the aquaculture environment is a critical measure for controlling <...
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MDPI AG
2024-12-01
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| Series: | Microorganisms |
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| author | Lijun Wang Bingwen Xi Kai Chen Jun Xie Liangkun Pan |
| author_facet | Lijun Wang Bingwen Xi Kai Chen Jun Xie Liangkun Pan |
| author_sort | Lijun Wang |
| collection | DOAJ |
| description | <i>Ichthyophthirius multifiliis</i>, a parasitic ciliate, causes “white spot disease” in freshwater fish and poses a significant threat to global freshwater aquaculture. Eliminating the free-swimming theront stage from the aquaculture environment is a critical measure for controlling <i>I. multifiliis</i> infections. The natural predator of <i>I. multifiliis</i> theronts in fish-farming ponds were identified using fluorescent dye-labelled live theronts and quantitative PCR; meanwhile, the zooplankton community composition in the positive ponds of <i>I. multifiliis</i> detected by quantitative PCR were analyzed by eDNA metabarcoding assay. The results revealed predation on theronts by cyclopoid copepods, including <i>Cyclops vicinus</i>, <i>Thermocyclops taihokuensis</i>, <i>Cyclops</i> sp., <i>Thermocyclops</i> sp., <i>Eucyclops</i> sp., and <i>Mesocyclops</i> sp. from the in-situ predation aquatic ecosystem, and among these copepods, <i>C. vicinus</i> was identified as a natural dominant predator of <i>I. multifiliis</i>. This study provides a scientific basis for further exploration and utilization of natural predators to enhance sustainable and environmentally friendly control strategies against <i>I. multifiliis</i>. |
| format | Article |
| id | doaj-art-94c137d81ff141db91c1466f26cde1fe |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-94c137d81ff141db91c1466f26cde1fe2025-01-24T13:42:23ZengMDPI AGMicroorganisms2076-26072024-12-011313810.3390/microorganisms13010038In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming PondLijun Wang0Bingwen Xi1Kai Chen2Jun Xie3Liangkun Pan4Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China<i>Ichthyophthirius multifiliis</i>, a parasitic ciliate, causes “white spot disease” in freshwater fish and poses a significant threat to global freshwater aquaculture. Eliminating the free-swimming theront stage from the aquaculture environment is a critical measure for controlling <i>I. multifiliis</i> infections. The natural predator of <i>I. multifiliis</i> theronts in fish-farming ponds were identified using fluorescent dye-labelled live theronts and quantitative PCR; meanwhile, the zooplankton community composition in the positive ponds of <i>I. multifiliis</i> detected by quantitative PCR were analyzed by eDNA metabarcoding assay. The results revealed predation on theronts by cyclopoid copepods, including <i>Cyclops vicinus</i>, <i>Thermocyclops taihokuensis</i>, <i>Cyclops</i> sp., <i>Thermocyclops</i> sp., <i>Eucyclops</i> sp., and <i>Mesocyclops</i> sp. from the in-situ predation aquatic ecosystem, and among these copepods, <i>C. vicinus</i> was identified as a natural dominant predator of <i>I. multifiliis</i>. This study provides a scientific basis for further exploration and utilization of natural predators to enhance sustainable and environmentally friendly control strategies against <i>I. multifiliis</i>.https://www.mdpi.com/2076-2607/13/1/38<i>Ichthyophthirius multifiliis</i><i>Cyclops vicinus</i>therontcopepodsbiocontrol |
| spellingShingle | Lijun Wang Bingwen Xi Kai Chen Jun Xie Liangkun Pan In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond Microorganisms <i>Ichthyophthirius multifiliis</i> <i>Cyclops vicinus</i> theront copepods biocontrol |
| title | In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond |
| title_full | In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond |
| title_fullStr | In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond |
| title_full_unstemmed | In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond |
| title_short | In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond |
| title_sort | in situ investigation of copepod predators of i ichthyophthirius multifiliis i theronts from fish farming pond |
| topic | <i>Ichthyophthirius multifiliis</i> <i>Cyclops vicinus</i> theront copepods biocontrol |
| url | https://www.mdpi.com/2076-2607/13/1/38 |
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