Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed
ABSTRACT: Skeletal muscle development is a complex, regulated physiological process that involves myoblast proliferation and differentiation and the fusion of myotubes. In this study, phenotypic differences in the breast and leg muscles of 180-day-old Jingyuan chickens were investigated. Differentia...
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912400871X |
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| author | Tong Zhang Jiwei Li Jiahuan Hu Yafei Chen Xi Fu Wei Zhao Jinli Tian Lijuan Yang Juan Zhang |
| author_facet | Tong Zhang Jiwei Li Jiahuan Hu Yafei Chen Xi Fu Wei Zhao Jinli Tian Lijuan Yang Juan Zhang |
| author_sort | Tong Zhang |
| collection | DOAJ |
| description | ABSTRACT: Skeletal muscle development is a complex, regulated physiological process that involves myoblast proliferation and differentiation and the fusion of myotubes. In this study, phenotypic differences in the breast and leg muscles of 180-day-old Jingyuan chickens were investigated. Differentially methylated genes (DMG) that regulate muscle development were identified through differential expression analysis and weighted gene co-expression network analysis. Moreover, myoblasts were used as test material and treated with cycloleucine to investigate the effect of N6-methyladenosine (m6A) modification on their proliferation and differentiation. The results revealed that the myofiber diameter and cross-sectional area in the breast muscle of Jingyuan chickens were significantly smaller than those in the leg muscle, while myofiber density in the breast muscle was significantly higher. A total of 484 DMG were identified in both muscle types. Module gene association analysis with DMGs revealed multiple DMG associated with muscle development. In vitro cell model analysis revealed that cycloleucine treatment significantly downregulated the m6A modification level of myoblasts and inhibited their proliferation and differentiation. Additionally, stage-specific differences in LDHA, LDHB, and GAPDH expressions were observed during myoblast differentiation. Cycloleucine treatment significantly inhibited LDHA, LDHB, and GAPDH expression. These findings indicate that m6A methylation modifications play significant regulatory roles in muscle development, with LDHA, LDHB, and GAPDH being potential candidate genes for regulating muscle development. This study provides an essential theoretical basis for further study on the functional mechanisms of m6A modifications involved in muscle development. |
| format | Article |
| id | doaj-art-94b9fdf1e81e46639455e6e35d286bf5 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-94b9fdf1e81e46639455e6e35d286bf52025-08-20T02:37:43ZengElsevierPoultry Science0032-57912024-12-011031210429210.1016/j.psj.2024.104292Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breedTong Zhang0Jiwei Li1Jiahuan Hu2Yafei Chen3Xi Fu4Wei Zhao5Jinli Tian6Lijuan Yang7Juan Zhang8College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCollege of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaCorresponding author; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaABSTRACT: Skeletal muscle development is a complex, regulated physiological process that involves myoblast proliferation and differentiation and the fusion of myotubes. In this study, phenotypic differences in the breast and leg muscles of 180-day-old Jingyuan chickens were investigated. Differentially methylated genes (DMG) that regulate muscle development were identified through differential expression analysis and weighted gene co-expression network analysis. Moreover, myoblasts were used as test material and treated with cycloleucine to investigate the effect of N6-methyladenosine (m6A) modification on their proliferation and differentiation. The results revealed that the myofiber diameter and cross-sectional area in the breast muscle of Jingyuan chickens were significantly smaller than those in the leg muscle, while myofiber density in the breast muscle was significantly higher. A total of 484 DMG were identified in both muscle types. Module gene association analysis with DMGs revealed multiple DMG associated with muscle development. In vitro cell model analysis revealed that cycloleucine treatment significantly downregulated the m6A modification level of myoblasts and inhibited their proliferation and differentiation. Additionally, stage-specific differences in LDHA, LDHB, and GAPDH expressions were observed during myoblast differentiation. Cycloleucine treatment significantly inhibited LDHA, LDHB, and GAPDH expression. These findings indicate that m6A methylation modifications play significant regulatory roles in muscle development, with LDHA, LDHB, and GAPDH being potential candidate genes for regulating muscle development. This study provides an essential theoretical basis for further study on the functional mechanisms of m6A modifications involved in muscle development.http://www.sciencedirect.com/science/article/pii/S003257912400871XJingyuan chickenN6-methyladenosineWGCNAmuscle development |
| spellingShingle | Tong Zhang Jiwei Li Jiahuan Hu Yafei Chen Xi Fu Wei Zhao Jinli Tian Lijuan Yang Juan Zhang Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed Poultry Science Jingyuan chicken N6-methyladenosine WGCNA muscle development |
| title | Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed |
| title_full | Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed |
| title_fullStr | Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed |
| title_full_unstemmed | Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed |
| title_short | Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed |
| title_sort | identification of key differentially methylated genes regulating muscle development in chickens insights from jingyuan breed |
| topic | Jingyuan chicken N6-methyladenosine WGCNA muscle development |
| url | http://www.sciencedirect.com/science/article/pii/S003257912400871X |
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