Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus
The green synthesis of silver-chitosan nanocomposite (AgCs) is a promising avenue in nanobiotechnology, offering eco-friendly production methods and multifunctional properties. This study elucidates the green synthesis of AgNp through a sustainable approach using Streptomyces griseoincarnatus RB7AG....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625000329 |
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| author | Subhransu Sekhar Behera Amrito Ghosh Majumdar Himansu Gullani Arka Lodh Roy Aruna Kumar Dash Pratyush Kumar Behera Zahra Parwez Seemon Giri Priti S. Mohanty Lopamudra Ray |
| author_facet | Subhransu Sekhar Behera Amrito Ghosh Majumdar Himansu Gullani Arka Lodh Roy Aruna Kumar Dash Pratyush Kumar Behera Zahra Parwez Seemon Giri Priti S. Mohanty Lopamudra Ray |
| author_sort | Subhransu Sekhar Behera |
| collection | DOAJ |
| description | The green synthesis of silver-chitosan nanocomposite (AgCs) is a promising avenue in nanobiotechnology, offering eco-friendly production methods and multifunctional properties. This study elucidates the green synthesis of AgNp through a sustainable approach using Streptomyces griseoincarnatus RB7AG. The synthesized AgNp was then combined with chitosan to form a silver-chitosan nanocomposite (AgCs nanocomposite) and investigated its properties employing UV–visible spectroscopy, FTIR techniques, and dynamic light scattering (DLS). The UV–visible spectroscopy confirms the successful formation of the nanocomposite, while DLS elucidates its size distribution and stability. Moreover, the antibiofilm capabilities of AgCs are evaluated against bacterial biofilms comprising Staphylococcus aureus and Escherichia coli. Remarkably, the nanocomposite demonstrates the most potent antibiofilm activity, significantly reducing biofilm metabolic activity. This observation is further corroborated by fluorescence imaging, providing visual confirmation of the reduction in biofilm formation. These findings underscore the potential of AgCs as an effective antimicrobial agent against bacterial biofilms, with implications for combating biofilm-associated infections. Furthermore, the nanocomposite was found to be non-toxic to the HEK293 cell lines, demonstrating its effectiveness in further applications. |
| format | Article |
| id | doaj-art-94b5748ec5aa40288b50975ef54d1f32 |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-94b5748ec5aa40288b50975ef54d1f322025-08-20T03:42:56ZengElsevierThe Microbe2950-19462025-03-01610026410.1016/j.microb.2025.100264Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureusSubhransu Sekhar Behera0Amrito Ghosh Majumdar1Himansu Gullani2Arka Lodh Roy3Aruna Kumar Dash4Pratyush Kumar Behera5Zahra Parwez6Seemon Giri7Priti S. Mohanty8Lopamudra Ray9School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, IndiaSchool of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, India; School of Chemical Engineering, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, India; Correspondence to: School of Biotechnology and School of Chemical Technology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, India.School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, India; Correspondence to: School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, India.The green synthesis of silver-chitosan nanocomposite (AgCs) is a promising avenue in nanobiotechnology, offering eco-friendly production methods and multifunctional properties. This study elucidates the green synthesis of AgNp through a sustainable approach using Streptomyces griseoincarnatus RB7AG. The synthesized AgNp was then combined with chitosan to form a silver-chitosan nanocomposite (AgCs nanocomposite) and investigated its properties employing UV–visible spectroscopy, FTIR techniques, and dynamic light scattering (DLS). The UV–visible spectroscopy confirms the successful formation of the nanocomposite, while DLS elucidates its size distribution and stability. Moreover, the antibiofilm capabilities of AgCs are evaluated against bacterial biofilms comprising Staphylococcus aureus and Escherichia coli. Remarkably, the nanocomposite demonstrates the most potent antibiofilm activity, significantly reducing biofilm metabolic activity. This observation is further corroborated by fluorescence imaging, providing visual confirmation of the reduction in biofilm formation. These findings underscore the potential of AgCs as an effective antimicrobial agent against bacterial biofilms, with implications for combating biofilm-associated infections. Furthermore, the nanocomposite was found to be non-toxic to the HEK293 cell lines, demonstrating its effectiveness in further applications.http://www.sciencedirect.com/science/article/pii/S2950194625000329AntimicrobialBiofilmE. coliSilver-chitosan nanocompositeStaphylococcus aureus |
| spellingShingle | Subhransu Sekhar Behera Amrito Ghosh Majumdar Himansu Gullani Arka Lodh Roy Aruna Kumar Dash Pratyush Kumar Behera Zahra Parwez Seemon Giri Priti S. Mohanty Lopamudra Ray Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus The Microbe Antimicrobial Biofilm E. coli Silver-chitosan nanocomposite Staphylococcus aureus |
| title | Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus |
| title_full | Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus |
| title_fullStr | Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus |
| title_full_unstemmed | Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus |
| title_short | Green synthesis of silver-chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria Escherichia coli and Staphylococcus aureus |
| title_sort | green synthesis of silver chitosan nanocomposite exhibits promising antibiofilm properties against pathogenic bacteria escherichia coli and staphylococcus aureus |
| topic | Antimicrobial Biofilm E. coli Silver-chitosan nanocomposite Staphylococcus aureus |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625000329 |
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