Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of w...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2013/482765 |
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| author | Tomoo Kudo Yoshihito Shimazu Hisao Yagishita Toshiyuki Izumo Yuuichi Soeno Kaori Sato Yuji Taya Takaaki Aoba |
| author_facet | Tomoo Kudo Yoshihito Shimazu Hisao Yagishita Toshiyuki Izumo Yuuichi Soeno Kaori Sato Yuji Taya Takaaki Aoba |
| author_sort | Tomoo Kudo |
| collection | DOAJ |
| description | We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4 μm thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications. |
| format | Article |
| id | doaj-art-94b4b1905d3e4041a9361e475cd30dbf |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-94b4b1905d3e4041a9361e475cd30dbf2025-02-03T01:10:10ZengWileyInternational Journal of Dentistry1687-87281687-87362013-01-01201310.1155/2013/482765482765Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion FrontTomoo Kudo0Yoshihito Shimazu1Hisao Yagishita2Toshiyuki Izumo3Yuuichi Soeno4Kaori Sato5Yuji Taya6Takaaki Aoba7Department of Pathology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Diagnostic Oral Pathology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 133-8549, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanWe conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4 μm thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications.http://dx.doi.org/10.1155/2013/482765 |
| spellingShingle | Tomoo Kudo Yoshihito Shimazu Hisao Yagishita Toshiyuki Izumo Yuuichi Soeno Kaori Sato Yuji Taya Takaaki Aoba Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front International Journal of Dentistry |
| title | Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front |
| title_full | Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front |
| title_fullStr | Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front |
| title_full_unstemmed | Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front |
| title_short | Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front |
| title_sort | three dimensional reconstruction of oral tongue squamous cell carcinoma at invasion front |
| url | http://dx.doi.org/10.1155/2013/482765 |
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