Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi
In this study, using Congo red staining method and enzyme activity assay, the high-yield cellulase fungi from soil samples in Turpan area was screened. The screened strain was identified by morphological observation and molecular biology techniques, and the enzymatic characteristics of cellulase pro...
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Editorial Department of China Brewing
2025-05-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-126.pdf |
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| author | LI Mei, LI Xue, FENG Yulian, JIANG Xingtong, TANG Qiyong, ZHANG Zhidong |
| author_facet | LI Mei, LI Xue, FENG Yulian, JIANG Xingtong, TANG Qiyong, ZHANG Zhidong |
| author_sort | LI Mei, LI Xue, FENG Yulian, JIANG Xingtong, TANG Qiyong, ZHANG Zhidong |
| collection | DOAJ |
| description | In this study, using Congo red staining method and enzyme activity assay, the high-yield cellulase fungi from soil samples in Turpan area was screened. The screened strain was identified by morphological observation and molecular biology techniques, and the enzymatic characteristics of cellulase produced by the strain were studied. The results showed that a high-yield cellulase fungi 12-3-6 was obtained, and the activity of filter paper enzyme (FPA) and carboxymethyl cellulase (CMCase) in the fermentation broth of the fungi were 7.30 U/ml and 39.56 U/ml, respectively. Strain 12-3-6 was identified as Aspergillus egyptiacus. The results of enzymatic characteristic analysis showed that the optimal reaction conditions of FPA and CMCase produced by strain 12-3-6 fermentation was both reaction temperature 50 ℃ and reaction pH value 5.0. The relative enzyme activity of FPA and CMCase produced by strain 12-3-6 fermentation was still higher after 1 h at 30-60 ℃ or pH 5.0-6.0, and Cu<sup>2+</sup> could significantly improve the enzyme activity. Sodium dodecyl sulfate (SDS) had a certain inhibitory effect on enzyme activity. The results indicated that the cellulase produced by strain 12-3-6 had high activity and stable enzymatic properties, and had a good application prospect. |
| format | Article |
| id | doaj-art-94a835fe7d0d487986c70a59ada90bf8 |
| institution | Kabale University |
| issn | 0254-5071 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-94a835fe7d0d487986c70a59ada90bf82025-08-20T03:29:56ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-05-0144512613110.11882/j.issn.0254-5071.2025.05.019Isolation, identification and enzymatic characteristics of a high-yield cellulase fungiLI Mei, LI Xue, FENG Yulian, JIANG Xingtong, TANG Qiyong, ZHANG Zhidong01. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China; ;2. Xinjiang Key Laboratory of Special Environmental Microbiology, Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; ;3. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; ;4. College of Life Sciences, Xinjiang Normal University, Urumqi 830054, ChinaIn this study, using Congo red staining method and enzyme activity assay, the high-yield cellulase fungi from soil samples in Turpan area was screened. The screened strain was identified by morphological observation and molecular biology techniques, and the enzymatic characteristics of cellulase produced by the strain were studied. The results showed that a high-yield cellulase fungi 12-3-6 was obtained, and the activity of filter paper enzyme (FPA) and carboxymethyl cellulase (CMCase) in the fermentation broth of the fungi were 7.30 U/ml and 39.56 U/ml, respectively. Strain 12-3-6 was identified as Aspergillus egyptiacus. The results of enzymatic characteristic analysis showed that the optimal reaction conditions of FPA and CMCase produced by strain 12-3-6 fermentation was both reaction temperature 50 ℃ and reaction pH value 5.0. The relative enzyme activity of FPA and CMCase produced by strain 12-3-6 fermentation was still higher after 1 h at 30-60 ℃ or pH 5.0-6.0, and Cu<sup>2+</sup> could significantly improve the enzyme activity. Sodium dodecyl sulfate (SDS) had a certain inhibitory effect on enzyme activity. The results indicated that the cellulase produced by strain 12-3-6 had high activity and stable enzymatic properties, and had a good application prospect.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-126.pdfcellulase|fungi|screen|identification|enzymatic characteristic |
| spellingShingle | LI Mei, LI Xue, FENG Yulian, JIANG Xingtong, TANG Qiyong, ZHANG Zhidong Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi Zhongguo niangzao cellulase|fungi|screen|identification|enzymatic characteristic |
| title | Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi |
| title_full | Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi |
| title_fullStr | Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi |
| title_full_unstemmed | Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi |
| title_short | Isolation, identification and enzymatic characteristics of a high-yield cellulase fungi |
| title_sort | isolation identification and enzymatic characteristics of a high yield cellulase fungi |
| topic | cellulase|fungi|screen|identification|enzymatic characteristic |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-5-126.pdf |
| work_keys_str_mv | AT limeilixuefengyulianjiangxingtongtangqiyongzhangzhidong isolationidentificationandenzymaticcharacteristicsofahighyieldcellulasefungi |