Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects

The large pine weevil (<i>Hylobius abietis</i> L.) is a major pest in European and Asian coniferous forests, particularly in managed plantations where clear-felling practices create ideal conditions for its population growth. Traditional management practices involving synthetic insectici...

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Main Authors: Luis M. Quinzo-Ortega, William T. Swaney, Roger Moore, Robbie Rae, Christopher D. Williams
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/15/12/967
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author Luis M. Quinzo-Ortega
William T. Swaney
Roger Moore
Robbie Rae
Christopher D. Williams
author_facet Luis M. Quinzo-Ortega
William T. Swaney
Roger Moore
Robbie Rae
Christopher D. Williams
author_sort Luis M. Quinzo-Ortega
collection DOAJ
description The large pine weevil (<i>Hylobius abietis</i> L.) is a major pest in European and Asian coniferous forests, particularly in managed plantations where clear-felling practices create ideal conditions for its population growth. Traditional management practices involving synthetic insecticides have limited efficacy in terms of reducing pest populations and pose environmental risks. This study evaluated the effectiveness of a wild entomopathogenic fungus (EPF) and the commercial entomopathogenic nematode <i>Steinernema carpocapsae</i> (EPN) as biological control agents (BCAs) against <i>H. abietis</i> in clear-felled spruce plantations in Wales and Scotland. Field trials used a randomised block design with three treatments (EPN full dose, EPF full dose and a combination of EPF+EPN at half doses each) compared to a control. Emergence trapping and destructive sampling were employed to assess treatment efficacy. All treatments significantly reduced weevil emergence, with the mixed treatment showing the greatest impact. Destructive sampling revealed strong associations between treatment type and infection outcomes in <i>H. abietis</i>, with a small but significant relationship between weevil developmental stages and infection types. Importantly, the treatments had no significant impact on the total abundance or taxon richness of non-target invertebrates. These findings suggest that wild EPFs alone and combined with EPNs are effective and environmentally safe alternatives to synthetic insecticides for managing <i>H. abietis</i> populations in managed forests.
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spelling doaj-art-949c088507ba4e1387abe05dda6d050a2025-08-20T02:00:34ZengMDPI AGInsects2075-44502024-12-01151296710.3390/insects15120967Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target EffectsLuis M. Quinzo-Ortega0William T. Swaney1Roger Moore2Robbie Rae3Christopher D. Williams4School of Biological and Environmental Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UKSchool of Biological and Environmental Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UKEntomology, Tree Health, Forest Research, Northern Research Station, Roslin, Midlothian EH25 9SY, UKSchool of Biological and Environmental Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UKSchool of Biological and Environmental Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UKThe large pine weevil (<i>Hylobius abietis</i> L.) is a major pest in European and Asian coniferous forests, particularly in managed plantations where clear-felling practices create ideal conditions for its population growth. Traditional management practices involving synthetic insecticides have limited efficacy in terms of reducing pest populations and pose environmental risks. This study evaluated the effectiveness of a wild entomopathogenic fungus (EPF) and the commercial entomopathogenic nematode <i>Steinernema carpocapsae</i> (EPN) as biological control agents (BCAs) against <i>H. abietis</i> in clear-felled spruce plantations in Wales and Scotland. Field trials used a randomised block design with three treatments (EPN full dose, EPF full dose and a combination of EPF+EPN at half doses each) compared to a control. Emergence trapping and destructive sampling were employed to assess treatment efficacy. All treatments significantly reduced weevil emergence, with the mixed treatment showing the greatest impact. Destructive sampling revealed strong associations between treatment type and infection outcomes in <i>H. abietis</i>, with a small but significant relationship between weevil developmental stages and infection types. Importantly, the treatments had no significant impact on the total abundance or taxon richness of non-target invertebrates. These findings suggest that wild EPFs alone and combined with EPNs are effective and environmentally safe alternatives to synthetic insecticides for managing <i>H. abietis</i> populations in managed forests.https://www.mdpi.com/2075-4450/15/12/967large pine weevilentomopathogenic nematodesentomopathogenic fungi
spellingShingle Luis M. Quinzo-Ortega
William T. Swaney
Roger Moore
Robbie Rae
Christopher D. Williams
Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
Insects
large pine weevil
entomopathogenic nematodes
entomopathogenic fungi
title Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
title_full Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
title_fullStr Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
title_full_unstemmed Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
title_short Field Trials of Wild Entomopathogenic Fungi and Commercial <i>Steinernema carpocapsae</i> on the Large Pine Weevil (<i>Hylobius abietis</i> [L.]) Including an Assessment of Non-Target Effects
title_sort field trials of wild entomopathogenic fungi and commercial i steinernema carpocapsae i on the large pine weevil i hylobius abietis i l including an assessment of non target effects
topic large pine weevil
entomopathogenic nematodes
entomopathogenic fungi
url https://www.mdpi.com/2075-4450/15/12/967
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