Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis
Background: The most prevalent mycosis in women with complex recurrence and tolerance to antifungals is vulvovaginal candidiasis (VVC), so more efficient therapies are needed. This study evaluated the antifungal efficiency of Origanum vulgare essential oil (O. vulgare EO) and its genetic influence o...
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Zagazig University, Faculty of Medicine
2024-11-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_383282_97b9fd1cf23e958cc3f45bd403e2273c.pdf |
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| author | Doaallah Diab Marwa Fathy Nashwa Ali Soha Elhady Yasmin Ahmed |
| author_facet | Doaallah Diab Marwa Fathy Nashwa Ali Soha Elhady Yasmin Ahmed |
| author_sort | Doaallah Diab |
| collection | DOAJ |
| description | Background: The most prevalent mycosis in women with complex recurrence and tolerance to antifungals is vulvovaginal candidiasis (VVC), so more efficient therapies are needed. This study evaluated the antifungal efficiency of Origanum vulgare essential oil (O. vulgare EO) and its genetic influence on CDR1 efflux pump gene among azole resistant Candida albicans (C. albicans). Methods: C. albicans was isolated from vaginal swabs. Antifungal effect of O. vulgare EO was determined in comparison to azole anti-fungal drugs against 30 vaginal C. albicans isolates using disk diffusion method. The MIC of fluconazole and O. vulgare EO was determined by microdilution method, while overexpression of efflux pump CDR1 gene among azole resistant C. albicans and effect of O. vulgare EO on gene expression was detected by quantitative real time PCR. Results: C. albicans accounted for (76.9%) of the isolated species, with C. glabrata following with (7.7%). Of all cases, (34%) were recurrent VVC, of which (53.8%) were non-albicans candida. (36.7%) of C. albicans isolates were resistant to one or more member of antifungal azoles, including (10%) isolates were resistant to all tested antifungal azoles. Regarding the group of tested azoles, resistant isolates were 16.7%, 13.4% and 10% for clotrimazole, itraconazole and fluconazole, respectively. (16.7%) of C. albicans were found to be resistant to fluconazole using the broth microdilution method. Disk diffusion testing revealed that all C. albicans isolates were sensitive to O. vulgare EO, with MIC values ranging from 0.125 to 0.007% v/v. (73%) of the isolates of azole-resistant C. albicans had overexpressed the CDR1 gene. In all azole-resistant C. albicans isolate with CDR1 gene over-expression, O. vulgare EO significantly reduced CDR1 gene expression (P-value=0.012). Conclusion: O. vulgare EO could act as an effective natural antifungal with potential utilization in the combat against azole resistant C. albicans. |
| format | Article |
| id | doaj-art-9498f2acb7864827808b37945116b00b |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj-art-9498f2acb7864827808b37945116b00b2024-12-21T21:03:54ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-11-01541654166610.21608/mid.2024.316938.2184383282Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasisDoaallah Diab0Marwa Fathy1Nashwa Ali2Soha Elhady3Yasmin Ahmed4Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, EgyptMedical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, EgyptObstetrics and Gynecology, Faculty of Medicine, Ain Shams University, EgyptMedical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, EgyptMedical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, EgyptBackground: The most prevalent mycosis in women with complex recurrence and tolerance to antifungals is vulvovaginal candidiasis (VVC), so more efficient therapies are needed. This study evaluated the antifungal efficiency of Origanum vulgare essential oil (O. vulgare EO) and its genetic influence on CDR1 efflux pump gene among azole resistant Candida albicans (C. albicans). Methods: C. albicans was isolated from vaginal swabs. Antifungal effect of O. vulgare EO was determined in comparison to azole anti-fungal drugs against 30 vaginal C. albicans isolates using disk diffusion method. The MIC of fluconazole and O. vulgare EO was determined by microdilution method, while overexpression of efflux pump CDR1 gene among azole resistant C. albicans and effect of O. vulgare EO on gene expression was detected by quantitative real time PCR. Results: C. albicans accounted for (76.9%) of the isolated species, with C. glabrata following with (7.7%). Of all cases, (34%) were recurrent VVC, of which (53.8%) were non-albicans candida. (36.7%) of C. albicans isolates were resistant to one or more member of antifungal azoles, including (10%) isolates were resistant to all tested antifungal azoles. Regarding the group of tested azoles, resistant isolates were 16.7%, 13.4% and 10% for clotrimazole, itraconazole and fluconazole, respectively. (16.7%) of C. albicans were found to be resistant to fluconazole using the broth microdilution method. Disk diffusion testing revealed that all C. albicans isolates were sensitive to O. vulgare EO, with MIC values ranging from 0.125 to 0.007% v/v. (73%) of the isolates of azole-resistant C. albicans had overexpressed the CDR1 gene. In all azole-resistant C. albicans isolate with CDR1 gene over-expression, O. vulgare EO significantly reduced CDR1 gene expression (P-value=0.012). Conclusion: O. vulgare EO could act as an effective natural antifungal with potential utilization in the combat against azole resistant C. albicans.https://mid.journals.ekb.eg/article_383282_97b9fd1cf23e958cc3f45bd403e2273c.pdfvulvovaginal candidiasisoriganum vulgare essential oilantifungal azole agentsefflux pump cdr1 geneazole resistant c. albicans |
| spellingShingle | Doaallah Diab Marwa Fathy Nashwa Ali Soha Elhady Yasmin Ahmed Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis Microbes and Infectious Diseases vulvovaginal candidiasis origanum vulgare essential oil antifungal azole agents efflux pump cdr1 gene azole resistant c. albicans |
| title | Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis |
| title_full | Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis |
| title_fullStr | Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis |
| title_full_unstemmed | Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis |
| title_short | Evaluation of antifungal activity of Origanum vulgare essential oil and detection of its influence on CDR1 gene expression among Candida albicans in vulvovaginal candidiasis |
| title_sort | evaluation of antifungal activity of origanum vulgare essential oil and detection of its influence on cdr1 gene expression among candida albicans in vulvovaginal candidiasis |
| topic | vulvovaginal candidiasis origanum vulgare essential oil antifungal azole agents efflux pump cdr1 gene azole resistant c. albicans |
| url | https://mid.journals.ekb.eg/article_383282_97b9fd1cf23e958cc3f45bd403e2273c.pdf |
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