Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons
Summary: We are reporting powerful streams of chromatin rupturing the nuclear envelope (NE) and the plasma membrane of migrating cerebral neurons in mouse embryos, which we suggest naming “piercing nuclear hernia” (PNH). About 40% of migrating neurons in cannabinoid type 1 receptor knock-out (CB1R−/...
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Elsevier
2025-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422501449X |
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| author | Yury M. Morozov Pasko Rakic |
| author_facet | Yury M. Morozov Pasko Rakic |
| author_sort | Yury M. Morozov |
| collection | DOAJ |
| description | Summary: We are reporting powerful streams of chromatin rupturing the nuclear envelope (NE) and the plasma membrane of migrating cerebral neurons in mouse embryos, which we suggest naming “piercing nuclear hernia” (PNH). About 40% of migrating neurons in cannabinoid type 1 receptor knock-out (CB1R−/−) mouse embryos and in wildtype embryos exposed to CB1R agonists show NE rupture and/or PNH. This indicates that deviations from optimal functioning of the endocannabinoid system in under- or over-activity may trigger analogous mechanisms increasing intranuclear pressure and chromatin herniation. The cells from CB1R−/− embryos showed pronounced ultrastructural disorders, such as high volume of herniated chromatin, mitochondrial fission, and negative correlation of the mitochondrial length with the volume of herniated chromatin. Catastrophic rupture of the nuclear and plasma membranes may provoke accidental cell death. At the same time, a fraction of neurons with PNH showed generally normal ultrastructure, which could indicate a mechanism of cell body repair. |
| format | Article |
| id | doaj-art-9495c03f1bc44cf9bfaf8b7928f14bc5 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-9495c03f1bc44cf9bfaf8b7928f14bc52025-08-20T04:00:34ZengElsevieriScience2589-00422025-08-0128811318810.1016/j.isci.2025.113188Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neuronsYury M. Morozov0Pasko Rakic1Department of Neuroscience, Yale University School of Medicine and Kavli Institute for Neuroscience, New Haven, CT 06510, USA; Corresponding authorDepartment of Neuroscience, Yale University School of Medicine and Kavli Institute for Neuroscience, New Haven, CT 06510, USASummary: We are reporting powerful streams of chromatin rupturing the nuclear envelope (NE) and the plasma membrane of migrating cerebral neurons in mouse embryos, which we suggest naming “piercing nuclear hernia” (PNH). About 40% of migrating neurons in cannabinoid type 1 receptor knock-out (CB1R−/−) mouse embryos and in wildtype embryos exposed to CB1R agonists show NE rupture and/or PNH. This indicates that deviations from optimal functioning of the endocannabinoid system in under- or over-activity may trigger analogous mechanisms increasing intranuclear pressure and chromatin herniation. The cells from CB1R−/− embryos showed pronounced ultrastructural disorders, such as high volume of herniated chromatin, mitochondrial fission, and negative correlation of the mitochondrial length with the volume of herniated chromatin. Catastrophic rupture of the nuclear and plasma membranes may provoke accidental cell death. At the same time, a fraction of neurons with PNH showed generally normal ultrastructure, which could indicate a mechanism of cell body repair.http://www.sciencedirect.com/science/article/pii/S258900422501449XBiological sciencesCellular neuroscienceDevelopmental neuroscienceNatural sciencesNeuroscience |
| spellingShingle | Yury M. Morozov Pasko Rakic Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons iScience Biological sciences Cellular neuroscience Developmental neuroscience Natural sciences Neuroscience |
| title | Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| title_full | Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| title_fullStr | Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| title_full_unstemmed | Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| title_short | Abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| title_sort | abnormalities of the endocannabinoid system produce piercing nuclear hernias in migrating cerebral neurons |
| topic | Biological sciences Cellular neuroscience Developmental neuroscience Natural sciences Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S258900422501449X |
| work_keys_str_mv | AT yurymmorozov abnormalitiesoftheendocannabinoidsystemproducepiercingnuclearherniasinmigratingcerebralneurons AT paskorakic abnormalitiesoftheendocannabinoidsystemproducepiercingnuclearherniasinmigratingcerebralneurons |