A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production

The objective of this study was to develop an efficient method for enhancing the production of bioethanol in a single-reactor system (SRS) by implementing acid–base pretreatment and enzymatic hydrolysis techniques, thereby eliminating differentiation and removal processes. The aim was to establish a...

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Main Authors: Nibedita Deb, Tawfkur Rahman, Md. Zahangir Alam, Md. Shohidullah Miah, Reashad Kamal
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:International Journal of Polymer Science
Online Access:http://dx.doi.org/10.1155/2024/5264918
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author Nibedita Deb
Tawfkur Rahman
Md. Zahangir Alam
Md. Shohidullah Miah
Reashad Kamal
author_facet Nibedita Deb
Tawfkur Rahman
Md. Zahangir Alam
Md. Shohidullah Miah
Reashad Kamal
author_sort Nibedita Deb
collection DOAJ
description The objective of this study was to develop an efficient method for enhancing the production of bioethanol in a single-reactor system (SRS) by implementing acid–base pretreatment and enzymatic hydrolysis techniques, thereby eliminating differentiation and removal processes. The aim was to establish a process for bioethanol synthesis using hydrolyzed palm oil mill effluent (POME) and a locally sourced cellulase enzyme. The pretreatment and enzymatic hydrolysis methods were successfully optimized, resulting in a maximum yield of reducing sugars of 26.6 g/L (53.14%). To achieve the highest bioethanol yield, fermentation was carried out using both the one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD) approaches. The OFAT approach was employed to obtain the maximum bioethanol production, which yielded 6.75% v/v of bioethanol from the hydrolyzed POME using the same bioreactor. In the case of the FCCCD process, the optimal conditions led to a bioethanol production of 7.64% v/v during the fermentation stage. Kinetic analyses of the bioethanol produced revealed a specific growth rate (μ) of 0.198 h−1 and a specific product formation rate (qp) of 0.239 h−1 after 3 days of fermentation. These findings highlight a promising strategy for efficient management of POME through the production of biofuels, which could contribute to the economic growth of the country.
format Article
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institution Kabale University
issn 1687-9430
language English
publishDate 2024-01-01
publisher Wiley
record_format Article
series International Journal of Polymer Science
spelling doaj-art-94938f39ca5f446282b6593330f7c1242025-02-03T11:07:16ZengWileyInternational Journal of Polymer Science1687-94302024-01-01202410.1155/2024/5264918A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol ProductionNibedita Deb0Tawfkur Rahman1Md. Zahangir Alam2Md. Shohidullah Miah3Reashad Kamal4College of Agricultural SciencesDepartment of Electrical and Electronic EngineeringBioenvironmental Engineering Research Centre (BERC)College of Agricultural SciencesDepartment of PharmacyThe objective of this study was to develop an efficient method for enhancing the production of bioethanol in a single-reactor system (SRS) by implementing acid–base pretreatment and enzymatic hydrolysis techniques, thereby eliminating differentiation and removal processes. The aim was to establish a process for bioethanol synthesis using hydrolyzed palm oil mill effluent (POME) and a locally sourced cellulase enzyme. The pretreatment and enzymatic hydrolysis methods were successfully optimized, resulting in a maximum yield of reducing sugars of 26.6 g/L (53.14%). To achieve the highest bioethanol yield, fermentation was carried out using both the one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD) approaches. The OFAT approach was employed to obtain the maximum bioethanol production, which yielded 6.75% v/v of bioethanol from the hydrolyzed POME using the same bioreactor. In the case of the FCCCD process, the optimal conditions led to a bioethanol production of 7.64% v/v during the fermentation stage. Kinetic analyses of the bioethanol produced revealed a specific growth rate (μ) of 0.198 h−1 and a specific product formation rate (qp) of 0.239 h−1 after 3 days of fermentation. These findings highlight a promising strategy for efficient management of POME through the production of biofuels, which could contribute to the economic growth of the country.http://dx.doi.org/10.1155/2024/5264918
spellingShingle Nibedita Deb
Tawfkur Rahman
Md. Zahangir Alam
Md. Shohidullah Miah
Reashad Kamal
A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
International Journal of Polymer Science
title A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
title_full A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
title_fullStr A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
title_full_unstemmed A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
title_short A Single-Reactor System for Simultaneous Pretreatment and Fermentation of POME for Bioethanol Production
title_sort single reactor system for simultaneous pretreatment and fermentation of pome for bioethanol production
url http://dx.doi.org/10.1155/2024/5264918
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