Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs
This study aimed to isolate and screen canine-derived probiotics with excellent probiotic properties. Strain characterization was conducted using a combination of in vitro and in vivo probiotic characterization and safety assessments, as well as complete genome analysis. The results showed that <...
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2024-09-01
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| author | Yuanyuan Zhang Mengdi Zhao Yueyao Li Shuang Liang Xinkang Li Yi Wu Guangyu Li |
| author_facet | Yuanyuan Zhang Mengdi Zhao Yueyao Li Shuang Liang Xinkang Li Yi Wu Guangyu Li |
| author_sort | Yuanyuan Zhang |
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| description | This study aimed to isolate and screen canine-derived probiotics with excellent probiotic properties. Strain characterization was conducted using a combination of in vitro and in vivo probiotic characterization and safety assessments, as well as complete genome analysis. The results showed that <i>Limosilactobacillus reuteri</i> LRA7 exhibited excellent bacteriostatic and antioxidant activities. The survival rate at pH 2.5 was 79.98%, and the viable counts after exposure to gastrointestinal fluid and 0.5% bile salts were 7.77 log CFU/mL and 5.29 log CFU/mL, respectively. The bacterium also exhibited high hydrophobicity, self-coagulation, and high temperature tolerance, was negative for hemolysis, and was sensitive to clindamycin. In vivo studies in mice showed that the serum superoxide dismutase activity level was 53.69 U/mL higher in the MR group of mice compared to that of the control group, the malondialdehyde content was 0.53 nmol/mL lower in the HR group, and the highest jejunal V/C value was 4.11 ± 1.05 in the HR group (<i>p</i> < 0.05). The <i>L. reuteri</i> LRA7 gene is 2.021 megabases in size, contains one chromosome and one plasmid, and is annotated with 1978 functional genes. In conclusion, <i>L. reuteri</i> LRA7 has good probiotic potential and is safe. It can be used as an ideal probiotic candidate strain of canine origin. |
| format | Article |
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| issn | 2076-2607 |
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| spelling | doaj-art-947f7c84d2c447f383b0b580ac021fa22025-08-20T01:55:41ZengMDPI AGMicroorganisms2076-26072024-09-01129181110.3390/microorganisms12091811Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from DogsYuanyuan Zhang0Mengdi Zhao1Yueyao Li2Shuang Liang3Xinkang Li4Yi Wu5Guangyu Li6College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaThis study aimed to isolate and screen canine-derived probiotics with excellent probiotic properties. Strain characterization was conducted using a combination of in vitro and in vivo probiotic characterization and safety assessments, as well as complete genome analysis. The results showed that <i>Limosilactobacillus reuteri</i> LRA7 exhibited excellent bacteriostatic and antioxidant activities. The survival rate at pH 2.5 was 79.98%, and the viable counts after exposure to gastrointestinal fluid and 0.5% bile salts were 7.77 log CFU/mL and 5.29 log CFU/mL, respectively. The bacterium also exhibited high hydrophobicity, self-coagulation, and high temperature tolerance, was negative for hemolysis, and was sensitive to clindamycin. In vivo studies in mice showed that the serum superoxide dismutase activity level was 53.69 U/mL higher in the MR group of mice compared to that of the control group, the malondialdehyde content was 0.53 nmol/mL lower in the HR group, and the highest jejunal V/C value was 4.11 ± 1.05 in the HR group (<i>p</i> < 0.05). The <i>L. reuteri</i> LRA7 gene is 2.021 megabases in size, contains one chromosome and one plasmid, and is annotated with 1978 functional genes. In conclusion, <i>L. reuteri</i> LRA7 has good probiotic potential and is safe. It can be used as an ideal probiotic candidate strain of canine origin.https://www.mdpi.com/2076-2607/12/9/1811probiotics<i>Limosilactobacillus reuteri</i>dogsprobiotic propertieswhole-genome sequencing |
| spellingShingle | Yuanyuan Zhang Mengdi Zhao Yueyao Li Shuang Liang Xinkang Li Yi Wu Guangyu Li Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs Microorganisms probiotics <i>Limosilactobacillus reuteri</i> dogs probiotic properties whole-genome sequencing |
| title | Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs |
| title_full | Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs |
| title_fullStr | Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs |
| title_full_unstemmed | Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs |
| title_short | Potential Probiotic Properties and Complete Genome Analysis of <i>Limosilactobacillus reuteri</i> LRA7 from Dogs |
| title_sort | potential probiotic properties and complete genome analysis of i limosilactobacillus reuteri i lra7 from dogs |
| topic | probiotics <i>Limosilactobacillus reuteri</i> dogs probiotic properties whole-genome sequencing |
| url | https://www.mdpi.com/2076-2607/12/9/1811 |
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