circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis
Objective To investigate the regulation of miR-125b-5p/HMGB3 axis by circNHSL1 on the biological behavior of breast cancer cells. Methods The breast cancer cells T47D were divided into si-NC group, si-circNHSL1 group, miR-NC group, and miR-125b-5p mimic group and si-circNHSL1+miR-125b-5p inhibitor...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-12-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-12-1678.pdf |
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| author | LI Huan, XIE Xianxin |
| author_facet | LI Huan, XIE Xianxin |
| author_sort | LI Huan, XIE Xianxin |
| collection | DOAJ |
| description | Objective To investigate the regulation of miR-125b-5p/HMGB3 axis by circNHSL1 on the biological behavior of breast cancer cells. Methods The breast cancer cells T47D were divided into si-NC group, si-circNHSL1 group, miR-NC group, and miR-125b-5p mimic group and si-circNHSL1+miR-125b-5p inhibitor group. The expression of circNHSL1 and miR-125b-5p in breast cancer tissues and adjacent tissues was determined using RT-qPCR. Western blot was conducted to detect the protein expression of high mobility group protein 3 (HMGB3). Dual luciferase reporter gene assay was used to confirm the interaction between circNHSL1 and miR-125b-5p, miR-125b-5p and HMGB3. The proliferation rate was measured by CCK-8 method; the colony formation assay was applied to detect the colony formation numbers; flow cytometry was used to measure cell apoptosis rate; Transwell assay was used to detect the numbers of migratory and invasive cells. Results Compared with adjacent tissues, circNHSL1 and HMGB3 expression in breast cancer tissues was significantly increased (P＜0.05), while the expression of miR-125b-5p was significantly decreased (P＜0.05). miR-125b-5p directly bound to circNHSL1 and HMGB3, respectively. The circNHSL1 knockdown down regulated circNHSL1 and HMGB3 expression and upregulated miR-125b-5p expression in T47D cells (P＜0.05). After transfection with miR-125b-5p mimic, miR-125b-5p expression was up-regulated and HMGB3 expression was down-regulated (P＜0.05). Moreover, transfection of miR-125b-5p inhibitor reversed the effect of circNHSL1 silencing on the expression of miR-125b-5p and HMGB3. Low expression of circNHSL1 or high expression of miR-125b-5p increased the cell inhibition rate, colony formation and apoptosis rate of T47D and decreased the number of migratory and invasive cells (P＜0.05). Moreover, transfection of miR-125b-5p inhibitor saved the effect of circNHSL1 silencing on cell phenotypes (P＜0.05). Conclusions Interfering with circNHSL1 might promote cell apoptosis and inhibit cell proliferation, migration and invasion in breast cancer cell by up-regulating the miR-125b-5p/HMGB3 axis. |
| format | Article |
| id | doaj-art-94736fb4bd4a488ab61be7a1a20bfaf4 |
| institution | DOAJ |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-94736fb4bd4a488ab61be7a1a20bfaf42025-08-20T02:50:23ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252024-12-0144121678168410.16352/j.issn.1001-6325.2024.12.1678circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axisLI Huan, XIE Xianxin0Department of Breast Medicine, Liaoning Cancer Hospital,Shenyang 110042, ChinaObjective To investigate the regulation of miR-125b-5p/HMGB3 axis by circNHSL1 on the biological behavior of breast cancer cells. Methods The breast cancer cells T47D were divided into si-NC group, si-circNHSL1 group, miR-NC group, and miR-125b-5p mimic group and si-circNHSL1+miR-125b-5p inhibitor group. The expression of circNHSL1 and miR-125b-5p in breast cancer tissues and adjacent tissues was determined using RT-qPCR. Western blot was conducted to detect the protein expression of high mobility group protein 3 (HMGB3). Dual luciferase reporter gene assay was used to confirm the interaction between circNHSL1 and miR-125b-5p, miR-125b-5p and HMGB3. The proliferation rate was measured by CCK-8 method; the colony formation assay was applied to detect the colony formation numbers; flow cytometry was used to measure cell apoptosis rate; Transwell assay was used to detect the numbers of migratory and invasive cells. Results Compared with adjacent tissues, circNHSL1 and HMGB3 expression in breast cancer tissues was significantly increased (P＜0.05), while the expression of miR-125b-5p was significantly decreased (P＜0.05). miR-125b-5p directly bound to circNHSL1 and HMGB3, respectively. The circNHSL1 knockdown down regulated circNHSL1 and HMGB3 expression and upregulated miR-125b-5p expression in T47D cells (P＜0.05). After transfection with miR-125b-5p mimic, miR-125b-5p expression was up-regulated and HMGB3 expression was down-regulated (P＜0.05). Moreover, transfection of miR-125b-5p inhibitor reversed the effect of circNHSL1 silencing on the expression of miR-125b-5p and HMGB3. Low expression of circNHSL1 or high expression of miR-125b-5p increased the cell inhibition rate, colony formation and apoptosis rate of T47D and decreased the number of migratory and invasive cells (P＜0.05). Moreover, transfection of miR-125b-5p inhibitor saved the effect of circNHSL1 silencing on cell phenotypes (P＜0.05). Conclusions Interfering with circNHSL1 might promote cell apoptosis and inhibit cell proliferation, migration and invasion in breast cancer cell by up-regulating the miR-125b-5p/HMGB3 axis.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-12-1678.pdfbreast cancer|circnhsl1|mir-125b-5p|high mobility group protein 3(hmgb3) |
| spellingShingle | LI Huan, XIE Xianxin circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis Jichu yixue yu linchuang breast cancer|circnhsl1|mir-125b-5p|high mobility group protein 3(hmgb3) |
| title | circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis |
| title_full | circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis |
| title_fullStr | circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis |
| title_full_unstemmed | circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis |
| title_short | circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis |
| title_sort | circnhsl1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating mir 125b 5p hmgb3 axis |
| topic | breast cancer|circnhsl1|mir-125b-5p|high mobility group protein 3(hmgb3) |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2024-44-12-1678.pdf |
| work_keys_str_mv | AT lihuanxiexianxin circnhsl1inhibitsproliferationandpromotesapoptosisofhumanbreastcancercelllinethroughregulatingmir125b5phmgb3axis |