Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida.
Antimicrobial resistance is a global threat to both human and animal health, and transfer of resistance between these spheres is recognised as a key concern for all species. Selection for resistance at sub-inhibitory antimicrobial concentrations has been characterised for some bacteria-antimicrobial...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0327115 |
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| author | Abigail Hughes Ludovic Pelligand Dan I Andersson Andrew Mead |
| author_facet | Abigail Hughes Ludovic Pelligand Dan I Andersson Andrew Mead |
| author_sort | Abigail Hughes |
| collection | DOAJ |
| description | Antimicrobial resistance is a global threat to both human and animal health, and transfer of resistance between these spheres is recognised as a key concern for all species. Selection for resistance at sub-inhibitory antimicrobial concentrations has been characterised for some bacteria-antimicrobial combinations but there is little data from non-laboratory strains, and veterinary antimicrobials and bacterial species. Here, we demonstrate a minimum selective concentration of 0.06 mg/L (1/6 xMIC) for florfenicol in wild-type Pasteurella multocida, through competition experiments between a susceptible strain and a floR-resistant mutant. We also show that sub-inhibitory concentrations of florfenicol do not appear to significantly select for de novo resistance in P. multocida and present the challenges with adapting experimental protocols between bacterial species. These results have important implications for antimicrobial resistance selection at sub-inhibitory concentrations, method development for within-species differentiation in novel bacterial species, and application to policy regarding antimicrobial contamination in animal-feed. |
| format | Article |
| id | doaj-art-94716c7423a64cd680e7e79eb46873a3 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-94716c7423a64cd680e7e79eb46873a32025-08-20T02:35:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032711510.1371/journal.pone.0327115Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida.Abigail HughesLudovic PelligandDan I AnderssonAndrew MeadAntimicrobial resistance is a global threat to both human and animal health, and transfer of resistance between these spheres is recognised as a key concern for all species. Selection for resistance at sub-inhibitory antimicrobial concentrations has been characterised for some bacteria-antimicrobial combinations but there is little data from non-laboratory strains, and veterinary antimicrobials and bacterial species. Here, we demonstrate a minimum selective concentration of 0.06 mg/L (1/6 xMIC) for florfenicol in wild-type Pasteurella multocida, through competition experiments between a susceptible strain and a floR-resistant mutant. We also show that sub-inhibitory concentrations of florfenicol do not appear to significantly select for de novo resistance in P. multocida and present the challenges with adapting experimental protocols between bacterial species. These results have important implications for antimicrobial resistance selection at sub-inhibitory concentrations, method development for within-species differentiation in novel bacterial species, and application to policy regarding antimicrobial contamination in animal-feed.https://doi.org/10.1371/journal.pone.0327115 |
| spellingShingle | Abigail Hughes Ludovic Pelligand Dan I Andersson Andrew Mead Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. PLoS ONE |
| title | Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. |
| title_full | Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. |
| title_fullStr | Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. |
| title_full_unstemmed | Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. |
| title_short | Selection for florfenicol resistance at sub-MIC concentrations in Pasteurella multocida. |
| title_sort | selection for florfenicol resistance at sub mic concentrations in pasteurella multocida |
| url | https://doi.org/10.1371/journal.pone.0327115 |
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