4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus.
p62, an adapter protein involved in selective autophagy, is mainly found in the cytoplasm under normal conditions. Because p62 has nuclear localization signal (NLS) and a nuclear export signal, it has been suggested that p62 shuttles between the nucleus and cytoplasm. We studied the effect of 4-hydr...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0316558 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206804375666688 |
|---|---|
| author | Emi Kayama Ning Baoshuo Rinna Tatsuno Kanako Nishi Elsayed S I Mohammed Yumi Abiko Toru Yanagawa Satoru Takahashi Eiji Warabi |
| author_facet | Emi Kayama Ning Baoshuo Rinna Tatsuno Kanako Nishi Elsayed S I Mohammed Yumi Abiko Toru Yanagawa Satoru Takahashi Eiji Warabi |
| author_sort | Emi Kayama |
| collection | DOAJ |
| description | p62, an adapter protein involved in selective autophagy, is mainly found in the cytoplasm under normal conditions. Because p62 has nuclear localization signal (NLS) and a nuclear export signal, it has been suggested that p62 shuttles between the nucleus and cytoplasm. We studied the effect of 4-hydroxy-nonenal (4-HNE), an endogenous lipid peroxidation product, on intracellular p62 distribution in mouse embryonic fibroblasts. We found that treatment of 4-HNE causes p62 translocation from the cytoplasm to the nucleus. Further analysis revealed that 4-HNE directly binds to exportin-1 (Xpo1), essential protein for nuclear export of various proteins. Further analysis 4-HNE enhanced intranuclear EGFP-NLS-CL1 degradation in a p62-dependent manner. Our results suggest that 4-HNE changes p62 localization to the nucleus by inhibiting Xpo1 and might affect intranuclear protein quality control. |
| format | Article |
| id | doaj-art-946a5d7b945f442fab2dc821cb39cd58 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-946a5d7b945f442fab2dc821cb39cd582025-02-07T05:30:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031655810.1371/journal.pone.03165584-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus.Emi KayamaNing BaoshuoRinna TatsunoKanako NishiElsayed S I MohammedYumi AbikoToru YanagawaSatoru TakahashiEiji Warabip62, an adapter protein involved in selective autophagy, is mainly found in the cytoplasm under normal conditions. Because p62 has nuclear localization signal (NLS) and a nuclear export signal, it has been suggested that p62 shuttles between the nucleus and cytoplasm. We studied the effect of 4-hydroxy-nonenal (4-HNE), an endogenous lipid peroxidation product, on intracellular p62 distribution in mouse embryonic fibroblasts. We found that treatment of 4-HNE causes p62 translocation from the cytoplasm to the nucleus. Further analysis revealed that 4-HNE directly binds to exportin-1 (Xpo1), essential protein for nuclear export of various proteins. Further analysis 4-HNE enhanced intranuclear EGFP-NLS-CL1 degradation in a p62-dependent manner. Our results suggest that 4-HNE changes p62 localization to the nucleus by inhibiting Xpo1 and might affect intranuclear protein quality control.https://doi.org/10.1371/journal.pone.0316558 |
| spellingShingle | Emi Kayama Ning Baoshuo Rinna Tatsuno Kanako Nishi Elsayed S I Mohammed Yumi Abiko Toru Yanagawa Satoru Takahashi Eiji Warabi 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. PLoS ONE |
| title | 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. |
| title_full | 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. |
| title_fullStr | 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. |
| title_full_unstemmed | 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. |
| title_short | 4-Hydroxy-2-nonenal causes nuclear accumulation of p62 by inhibiting Xpo1 and promoting the proteolytic pathway in the nucleus. |
| title_sort | 4 hydroxy 2 nonenal causes nuclear accumulation of p62 by inhibiting xpo1 and promoting the proteolytic pathway in the nucleus |
| url | https://doi.org/10.1371/journal.pone.0316558 |
| work_keys_str_mv | AT emikayama 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT ningbaoshuo 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT rinnatatsuno 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT kanakonishi 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT elsayedsimohammed 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT yumiabiko 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT toruyanagawa 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT satorutakahashi 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus AT eijiwarabi 4hydroxy2nonenalcausesnuclearaccumulationofp62byinhibitingxpo1andpromotingtheproteolyticpathwayinthenucleus |