Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone
Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue i...
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2024-11-01
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| author | Mhd Safwan Albougha Hideki Sugii Orie Adachi Bara Mardini Serina Soeno Sayuri Hamano Daigaku Hasegawa Shinichiro Yoshida Tomohiro Itoyama Junko Obata Hidefumi Maeda |
| author_facet | Mhd Safwan Albougha Hideki Sugii Orie Adachi Bara Mardini Serina Soeno Sayuri Hamano Daigaku Hasegawa Shinichiro Yoshida Tomohiro Itoyama Junko Obata Hidefumi Maeda |
| author_sort | Mhd Safwan Albougha |
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| description | Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 ± 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (<i>ALP</i>), bone morphogenetic protein 2 (<i>BMP2</i>), osteocalcin (<i>OCN</i>), and osteopontin (<i>OPN</i>) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue. |
| format | Article |
| id | doaj-art-9463d319af7e427281bef5343e77fbe4 |
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| issn | 2218-273X |
| language | English |
| publishDate | 2024-11-01 |
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| series | Biomolecules |
| spelling | doaj-art-9463d319af7e427281bef5343e77fbe42025-08-20T02:08:02ZengMDPI AGBiomolecules2218-273X2024-11-011411145510.3390/biom14111455Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial BoneMhd Safwan Albougha0Hideki Sugii1Orie Adachi2Bara Mardini3Serina Soeno4Sayuri Hamano5Daigaku Hasegawa6Shinichiro Yoshida7Tomohiro Itoyama8Junko Obata9Hidefumi Maeda10Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDeep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 ± 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (<i>ALP</i>), bone morphogenetic protein 2 (<i>BMP2</i>), osteocalcin (<i>OCN</i>), and osteopontin (<i>OPN</i>) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue.https://www.mdpi.com/2218-273X/14/11/1455periodontal ligament stem cellsexosomesosteoblast-like cellsbone healing |
| spellingShingle | Mhd Safwan Albougha Hideki Sugii Orie Adachi Bara Mardini Serina Soeno Sayuri Hamano Daigaku Hasegawa Shinichiro Yoshida Tomohiro Itoyama Junko Obata Hidefumi Maeda Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone Biomolecules periodontal ligament stem cells exosomes osteoblast-like cells bone healing |
| title | Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone |
| title_full | Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone |
| title_fullStr | Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone |
| title_full_unstemmed | Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone |
| title_short | Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone |
| title_sort | exosomes from human periodontal ligament stem cells promote differentiation of osteoblast like cells and bone healing in rat calvarial bone |
| topic | periodontal ligament stem cells exosomes osteoblast-like cells bone healing |
| url | https://www.mdpi.com/2218-273X/14/11/1455 |
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