The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer
ABSTRACT The gut microbiota is intricately associated with the onset and progression of colorectal cancer (CRC), leading to significant interest in developing prevention and treatment strategies that leverage gut microbiota. In this study, we collected 57 samples from 19 CRC patients, comprising can...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2025-06-01
|
| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00198-25 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850100712708505600 |
|---|---|
| author | Lei Liu Jianguo Shi Hui Wang Hansong Du Jia Yang Kai Wei Zhuohui Zhou Moli Li Shuai Huang Lifang Zhan Guolong Li Yongling Lv Hexiao Shen Wei Cai |
| author_facet | Lei Liu Jianguo Shi Hui Wang Hansong Du Jia Yang Kai Wei Zhuohui Zhou Moli Li Shuai Huang Lifang Zhan Guolong Li Yongling Lv Hexiao Shen Wei Cai |
| author_sort | Lei Liu |
| collection | DOAJ |
| description | ABSTRACT The gut microbiota is intricately associated with the onset and progression of colorectal cancer (CRC), leading to significant interest in developing prevention and treatment strategies that leverage gut microbiota. In this study, we collected 57 samples from 19 CRC patients, comprising cancerous tissue, paracancerous tissue, and normal mucosa. Utilizing metagenomic sequencing and bioinformatics analysis, we identified differences in the microbiomes and their functional characteristics across the various tissue types. The results indicated that species such as Alistipes putredinis were predominantly found in normal tissues, while Pseudomonas putida was enriched in paracancerous tissue, and Malassezia restricta was prevalent in cancerous tissues. Furthermore, the microbial functions exhibited variability among the different tissue types. Random forest analysis suggested that Moraxella osloensis may be implicated in the onset and progression of colorectal cancer. We also classified the patients into three subgroups based on the anatomical location of the colorectum: right-sided colon, left-sided colon, and rectum. The subgroup analysis revealed that the microbiota enriched in normal mucosa and paracancerous tissue varied across different anatomical sites. These findings not only elucidate the characteristics of the microbiomes in the normal mucosa, paracancerous tissue, and cancerous tissues of CRC patients, thereby providing new potential targets for clinical diagnosis and treatment, but also contribute to the existing microbiome data pertinent to CRC research.IMPORTANCEThis study provides crucial insights into the relationship between gut microbiota and colorectal cancer (CRC) by analyzing microbial communities in different tissue types and anatomical locations of CRC patients. We identified distinct microbial signatures, such as Alistipes putredinis in normal tissues and Malassezia restricta in cancerous tissues, indicating location-specific microbiomes with unique functional attributes. These findings suggest potential new biomarkers or therapeutic targets for CRC. The observed microbiota variations among right-sided colon, left-sided colon, and rectum cancers underscore the heterogeneity of CRC, pointing toward more personalized treatment strategies. By enhancing our understanding of the microbiome’s role in CRC, this research paves the way for innovative diagnostic tools and targeted therapies tailored to individual patient profiles. This work is essential for advancing clinical approaches to CRC management. |
| format | Article |
| id | doaj-art-945f014ff8cf4ff08df079e51eb43972 |
| institution | DOAJ |
| issn | 2379-5077 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj-art-945f014ff8cf4ff08df079e51eb439722025-08-20T02:40:14ZengAmerican Society for MicrobiologymSystems2379-50772025-06-0110610.1128/msystems.00198-25The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancerLei Liu0Jianguo Shi1Hui Wang2Hansong Du3Jia Yang4Kai Wei5Zhuohui Zhou6Moli Li7Shuai Huang8Lifang Zhan9Guolong Li10Yongling Lv11Hexiao Shen12Wei Cai13Department of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaSchool of Life Sciences, Hubei University, Wuhan, Hubei, ChinaHubei Provincial Engineering Research Center of Intestinal Microecological Diagnostics, Therapeutics, and Clinical Translation, Wuhan, Hubei, ChinaHubei Provincial Engineering Research Center of Intestinal Microecological Diagnostics, Therapeutics, and Clinical Translation, Wuhan, Hubei, ChinaDepartment of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaABSTRACT The gut microbiota is intricately associated with the onset and progression of colorectal cancer (CRC), leading to significant interest in developing prevention and treatment strategies that leverage gut microbiota. In this study, we collected 57 samples from 19 CRC patients, comprising cancerous tissue, paracancerous tissue, and normal mucosa. Utilizing metagenomic sequencing and bioinformatics analysis, we identified differences in the microbiomes and their functional characteristics across the various tissue types. The results indicated that species such as Alistipes putredinis were predominantly found in normal tissues, while Pseudomonas putida was enriched in paracancerous tissue, and Malassezia restricta was prevalent in cancerous tissues. Furthermore, the microbial functions exhibited variability among the different tissue types. Random forest analysis suggested that Moraxella osloensis may be implicated in the onset and progression of colorectal cancer. We also classified the patients into three subgroups based on the anatomical location of the colorectum: right-sided colon, left-sided colon, and rectum. The subgroup analysis revealed that the microbiota enriched in normal mucosa and paracancerous tissue varied across different anatomical sites. These findings not only elucidate the characteristics of the microbiomes in the normal mucosa, paracancerous tissue, and cancerous tissues of CRC patients, thereby providing new potential targets for clinical diagnosis and treatment, but also contribute to the existing microbiome data pertinent to CRC research.IMPORTANCEThis study provides crucial insights into the relationship between gut microbiota and colorectal cancer (CRC) by analyzing microbial communities in different tissue types and anatomical locations of CRC patients. We identified distinct microbial signatures, such as Alistipes putredinis in normal tissues and Malassezia restricta in cancerous tissues, indicating location-specific microbiomes with unique functional attributes. These findings suggest potential new biomarkers or therapeutic targets for CRC. The observed microbiota variations among right-sided colon, left-sided colon, and rectum cancers underscore the heterogeneity of CRC, pointing toward more personalized treatment strategies. By enhancing our understanding of the microbiome’s role in CRC, this research paves the way for innovative diagnostic tools and targeted therapies tailored to individual patient profiles. This work is essential for advancing clinical approaches to CRC management.https://journals.asm.org/doi/10.1128/msystems.00198-25gut microbiotacolorectal cancerintratissue bacteriaright-sided colonmicrobiomefunctional analysis |
| spellingShingle | Lei Liu Jianguo Shi Hui Wang Hansong Du Jia Yang Kai Wei Zhuohui Zhou Moli Li Shuai Huang Lifang Zhan Guolong Li Yongling Lv Hexiao Shen Wei Cai The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer mSystems gut microbiota colorectal cancer intratissue bacteria right-sided colon microbiome functional analysis |
| title | The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| title_full | The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| title_fullStr | The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| title_full_unstemmed | The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| title_short | The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| title_sort | characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer |
| topic | gut microbiota colorectal cancer intratissue bacteria right-sided colon microbiome functional analysis |
| url | https://journals.asm.org/doi/10.1128/msystems.00198-25 |
| work_keys_str_mv | AT leiliu thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT jianguoshi thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT huiwang thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT hansongdu thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT jiayang thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT kaiwei thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT zhuohuizhou thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT molili thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT shuaihuang thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT lifangzhan thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT guolongli thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT yonglinglv thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT hexiaoshen thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT weicai thecharacteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT leiliu characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT jianguoshi characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT huiwang characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT hansongdu characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT jiayang characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT kaiwei characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT zhuohuizhou characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT molili characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT shuaihuang characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT lifangzhan characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT guolongli characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT yonglinglv characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT hexiaoshen characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer AT weicai characteristicsoftissuemicrobiotaindifferentanatomicallocationsanddifferenttissuetypesofthecolorectuminpatientswithcolorectalcancer |